Human Glutathione peroxidase 3 ELISA Kit | GPX3 elisa kit

Human Glutathione peroxidase 3 ELISA Kit

Cat. Num. AAA2881280

• Gene Names: GPX3; GPx-P; GSHPx-3; GSHPx-P
• Reactivity: Human
• Synonyms: Glutathione peroxidase 3; Human Glutathione peroxidase 3 ELISA Kit; GPx-3; GSHPx-3; Extracellular glutathione peroxidase; Plasma glutathione peroxidase; GPx-P; GSHPx-P; GPX3; GPXP; 1.11.1.9; GPX3 elisa kit

• Reactivity: Human
• Specificity: Recombinant and natural Human GPx-3
• Sequence Length: 226

• Samples: Serum, plasma, tissue homogenates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Competitive
• Detection Range: 1.56-100 ng/mL
• Sensitivity: < 0.23 ng/mL
• Intra-assay Precision: <=5.3%
• Inter-assay Precision: <=7.8%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GPX3 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen, During the reaction, target antigen in the sample or standard competes with a fixed amount of biotin-labeled target antigen for sites on a pre-coated antibody specific to target antigen. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 6006001
• NCBI GeneID: 2878
• NCBI Accession #: NP_002075.2
• NCBI GenBank Nucleotide #: NM_002084.3
• UniProt Accession #: P22352; O43787; Q86W78; Q9NZ74; Q9UEL1
• Molecular Weight: 25,552 Da
• NCBI Official Full Name: glutathione peroxidase 3
• NCBI Official Synonym Full Names: glutathione peroxidase 3
• NCBI Official Symbol: GPX3
• NCBI Official Synonym Symbols: GPx-P; GSHPx-3; GSHPx-P
• NCBI Protein Information: glutathione peroxidase 3
• UniProt Protein Name: Glutathione peroxidase 3
• Protein Family: Glutathione peroxidase
• UniProt Gene Name: GPX3
• UniProt Synonym Gene Names: GPXP; GPx-3; GSHPx-3; GPx-P; GSHPx-P
• UniProt Entry Name: GPX3_HUMAN

NCBI Description

This gene product belongs to the glutathione peroxidase family, which functions in the detoxification of hydrogen peroxide. It contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX3: Protects cells and enzymes from oxidative damage, by catalyzing the reduction of hydrogen peroxide, lipid peroxides and organic hydroperoxide, by glutathione. Belongs to the glutathione peroxidase family.

Protein type: Secreted, signal peptide; Secreted; Lipid Metabolism - arachidonic acid; Oxidoreductase; EC 1.11.1.9; Other Amino Acids Metabolism - glutathione

Chromosomal Location of Human Ortholog: 5q33.1

Cellular Component: extracellular region; extracellular space

Molecular Function: glutathione peroxidase activity; protein binding; selenium binding; transcription factor binding

Biological Process: hydrogen peroxide catabolic process; protein homotetramerization; response to lipid hydroperoxide; response to reactive oxygen species