SDS-Page
(Recombinant PBRM1 (23-156) protein gel. PBRM1 (23-156) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 22 kDa Purity: ? 90%)
PBRM1 recombinant protein
Recombinant PBRM1 (23-156) protein
Synonyms
PBRM1; Recombinant PBRM1 (23-156) protein; BRG1-associated factor 180 (BAF180); Polybromo 1; PB1; BAF180; PBRM1 recombinant protein
Host
E Coli
Form/Format
Recombinant PBRM1 (23-156) protein is supplied in 25mM Tris pH8.0, 500mM NaCl, 0.04% Triton X-100, 20% glycerol.
Protein Species
Human
Tag
DYKDDDDK-Tag, His-Tag
Notes
Recombinant PBRM1 (23-156) is suitable for use in binding assays, inhibitor screening, and selectivity profiling.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant PBRM1 (23-156) protein gel. PBRM1 (23-156) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 22 kDa Purity: ? 90%)
SDS-Page
(Recombinant PBRM1 (23-156) protein gel. PBRM1 (23-156) protein was run on a 12.5% SDS-PAGE gel and stained with Coomassie Blue. MW: 22 kDa Purity: ? 90%)
Testing Data
(HTRF Assay for Recombinant PBRM1 (23-156) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (23-156) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant PBRM1 (23-156) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (23-156) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant PBRM1 (23-156) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (23-156) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Testing Data
(HTRF Assay for Recombinant PBRM1 (23-156) protein activity. 3 uM histone peptide H4K5/8/12/16 (4Ac) was incubated with PBRM1 (23-156) protein in reaction buffer including 50mM HEPES-NaOH pH 7.0, 0.1% BSA overnight at room temperature. Anti-DYKDDDDK antibody was used to detect reaction products.)
Related Product Information for PBRM1 recombinant protein
Short Description: The peptide corresponding to amino acids 23-156 that contains the bromodomain sequences of PBRM1 (accession number NM_018165.4) was expressed in E Coli and contains an N-terminal His-tag and C-terminal DYKDDDDK tag with an observed molecular weight of 22 kDa. Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers.
Background: Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers.
Background: Polybromo 1 (PBRM1) protein, also known as BRG1-associated factor 180 (BAF180) is the chromatin targeting subunit of the SWI/SNF-B (PBAF) chromatin remodeling complex that is involved in transcriptional activation and repression of select genes. PBRM1 targets chromatin via its six bromodomains that recognize and bind acetylated histone lysine residues. Bromodomains function as 'readers' of these epigenetic histone marks and regulate chromatin structure and gene expression by linking associated proteins to the acetylated nucleosomal targets. PBRM1 also acts as an inhibitor of cell proliferation and demonstrates tumor suppressing properties in relation to breast, liver and lung cancers.
Product Categories/Family for PBRM1 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 22kDa
Protein Family
Similar Products
Product Notes
The PBRM1 (Catalog #AAA388103) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PBRM1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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