Testing Data
Eukaryotes Phosphoglycerate Dehydrogenase Assay Kit
Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric)
Reactivity
Eukaryotes
Synonyms
Phosphoglycerate Dehydrogenase; Phosphoglycerate Dehydrogenase (PHGDH) Activity Assay Kit (Colorimetric); Phosphoglycerate Dehydrogenase assay kit
Reactivity
Eukaryotes
Samples
Animal tissues: Liver and kidney, etc
Cell culture: Adherent or Suspension Cells
Cell culture: Adherent or Suspension Cells
Detection Method
Colorimetric: OD 450nm
Features & Benefits
Simple, rapid & convenient assay to measure PHGDH Activity in biological samples
Includes Positive Control
Measurement of PHGDH activity in purified/crude enzyme preparations and biological samples such as cell/tissue lysates
Includes Positive Control
Measurement of PHGDH activity in purified/crude enzyme preparations and biological samples such as cell/tissue lysates
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Phosphoglycerate Dehydrogenase assay kit
Phosphoglycerate Dehydrogenase (PHGDH: EC 1.1.1.95) is the first enzyme that participates in the L-serine synthesis pathway. PHGDH catalyzes the reversible reaction that converts 3-phosphoglycerate into 3-phosphohydroxyglycerate and the NADH-dependent reduction of alpha-ketoglutarate into D-2-hydroxyglutarate (D-2HG). In humans, PHGDH deficiency causes severe health problems including congenital microcephaly, psychomotor retardation, and intractable seizure. Recent studies have shown increased PHGDH activity in a wide variety of cancers, particularly in breast and melanoma tumors. Therefore, the measurement of PHGDH activity has become of great importance in searching potential therapeutic agents against cancer. Phosphoglycerate Dehydrogenase Assay kit provides a quick and easy method for monitoring PHGDH activity in a variety of samples. In this kit, Phosphoglycerate Dehydrogenase converts 3-phosphoglycerate and NAD into 3-phosphohydroxyglerate and NADH respectively. The oxidation of NADH reduces a probe generating a strong, stable absorbance signal (OD: 450 nm). The assay is simple, sensitive, high-throughput adaptable and can detect Phosphoglycerate Dehydrogenase activity less than 0.1 mU per sample.
Product Categories/Family for Phosphoglycerate Dehydrogenase assay kit
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Product Notes
The Eukaryotes Phosphoglycerate Dehydrogenase (Catalog #AAA849739) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849739 Assay Kit recognizes Eukaryotes Phosphoglycerate Dehydrogenase. It is sometimes possible for the material contained within the vial of "Phosphoglycerate Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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