Rabbit anti-Human TTL Polyclonal Antibody | anti-TTL antibody

TTL, CT (TTL, Tubulin-tyrosine ligase) (PE)

Cat. Num. AAA6360034

• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TTL; Polyclonal Antibody; CT (TTL; Tubulin-tyrosine ligase) (PE); Tubulin--tyrosine ligase; anti-TTL antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-TTL antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: TTL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 355-385 amino acids from the C-terminal region of human TTL.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(TTL Antibody (C-term) western blot analysis in Ramos cell line lysates (35ug/lane).This demonstrates the TTL antibody detected the TTL protein (arrow).)

Related Product Information for anti-TTL antibody

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).

Product Categories/Family for anti-TTL antibody

Antibodies; Tubulin

References

Dick, D.M., et al. Am. J. Med. Genet. B Neuropsychiatr. Genet. 153B (6), 1179-1188 (2010): Erck, C., et al. Cytogenet. Genome Res. 101(1):47-53(2003). Mialhe, A., et al. Cancer Res. 61(13):5024-5027(2001). Ersfeld, K., et al. J. Cell Biol. 120(3):725-732(1993).

NCBI and Uniprot Product Information

• NCBI GI #: 24308516
• NCBI GeneID: 150465
• NCBI Accession #: NP_714923.1
• NCBI GenBank Nucleotide #: NM_153712.4
• UniProt Accession #: Q8NG68; Q585T3; Q7Z302; Q8N426
• Molecular Weight: 43,212 Da
• NCBI Official Full Name: tubulin--tyrosine ligase
• NCBI Official Synonym Full Names: tubulin tyrosine ligase
• NCBI Official Symbol: TTL
• NCBI Protein Information: tubulin--tyrosine ligase; 2410003M22Rik; tubulin-tyrosine ligase
• UniProt Protein Name: Tubulin--tyrosine ligase
• Protein Family: TITAN-like protein
• UniProt Gene Name: TTL
• UniProt Synonym Gene Names: TTL
• UniProt Entry Name: TTL_HUMAN

NCBI Description

TTL is a cytosolic enzyme involved in the posttranslational modification of alpha-tubulin (see MIM 602529). Alpha-tubulin within assembled microtubules is detyrosinated over time at the C terminus. After microtubule disassembly, TTL restores the tyrosine residues and consequently participates in a cycle of tubulin detyrosination and tyrosination (Erck et al., 2003 [PubMed 14571137]).[supplied by OMIM, Mar 2008]

Uniprot Description

TTL: Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin. Belongs to the tubulin--tyrosine ligase family.

Protein type: EC 6.3.2.25; Cell development/differentiation; Ligase

Chromosomal Location of Human Ortholog: 2q13

Molecular Function: tubulin-tyrosine ligase activity; ATP binding

Biological Process: protein modification process; regulation of axon extension; microtubule cytoskeleton organization and biogenesis