General Sorbitol Dehydrogenase Assay Kit | SORD assay kit

Sorbitol Dehydrogenase Microplate Assay Kit

Cat. Num. AAA8243258

• Gene Names: SORD; SORD1; HEL-S-95n
• Reactivity: General
• Applications: Functional Assay
• Synonyms: Sorbitol Dehydrogenase; Sorbitol Dehydrogenase Microplate Assay Kit; EC 1.1.1.14; Sorbitol dehydrogenase; SDH; SORD; L-iditol 2-dehydrogenase; Glucitol dehydrogenase; Polyol dehydrogenase; SORD assay kit

• Reactivity: General
• Sequence Length: 357

• Applicable Applications for SORD assay kit: Functional Assay
• Samples: Urine, serum, plasma, tissue extracts, cell lysate, cell culture media and other biological fluids samples
• Detection Range: 4 umol/L - 400 umol/L
• Preparation and Storage: Shipped and store at 4 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SORD assay kit

Background/Introduction: Sorbitol dehydrogenase (SDH), found in organisms from bacteria to humans, converts sorbitol, the sugar alcohol form of glucose, to fructose, and the zinc-dependent reaction uses NAD+ as a cofactor. Organs abundant in SDH activity include the liver, kidney, lens and seminal vesicle. SDH and aldose reductase (AR) form the polyol pathway that interconverts glucose and fructose. Since SDH activity promotes the formation of NADH, the redox change induced by elevated SDH may have a pathogenic role in certain disease state, making SDH a potential therapeutic target. In addition, SDH has a close evolutionary relationship with alcohol dehydrogenase (ADH). The assay is initiated with the enzymatic hydrolysis of the sorbitol by sorbitol dehydrogenase. The enzyme catalysed reaction products NADH, can be measured at a colorimetric readout at 340 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 156627571
• NCBI GeneID: 6652
• NCBI Accession #: NP_003095.2
• NCBI GenBank Nucleotide #: NM_003104.5
• Molecular Weight: 10,812 Da
• NCBI Official Full Name: sorbitol dehydrogenase
• NCBI Official Synonym Full Names: sorbitol dehydrogenase
• NCBI Official Symbol: SORD
• NCBI Official Synonym Symbols: SORD1; HEL-S-95n
• NCBI Protein Information: sorbitol dehydrogenase
• UniProt Protein Name: Sorbitol dehydrogenase
• Protein Family: Sorbitol dehydrogenase
• UniProt Gene Name: SORD
• UniProt Entry Name: DHSO_HUMAN

NCBI Description

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

Uniprot Description

SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: Carbohydrate Metabolism - fructose and mannose; EC 1.1.1.14; Oxidoreductase

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: cytosol; extracellular space; membrane; mitochondrial membrane

Molecular Function: carbohydrate binding; D-xylulose reductase activity; identical protein binding; L-iditol 2-dehydrogenase activity; NAD binding; zinc ion binding

Biological Process: carbohydrate metabolic process; fructose biosynthetic process; fructose metabolic process; glucose metabolic process; glucuronate catabolic process to xylulose 5-phosphate; L-xylitol catabolic process; L-xylitol metabolic process; response to cadmium ion; response to copper ion; response to drug; response to hormone stimulus; response to nutrient levels; response to osmotic stress; sorbitol catabolic process; sperm motility