Typical Testing Data/Standard Curve (for reference only)
beta-N-Acetylglucosaminidase Assay Kit
beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric)
Synonyms
beta-N-Acetylglucosaminidase; beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric); NAG Activity Assay Kit (Colorimetric); beta-N-Acetylglucosaminidase assay kit
Sequence Length
564
Application Notes
Measurement of NAG in biological samples from different mammalian species
Samples
Biological fluids such as urine, serum etc.
Tissues such as kidney, liver, brain etc.
Cells such as HeLa, HepG2, CHO etc.
Tissues such as kidney, liver, brain etc.
Cells such as HeLa, HepG2, CHO etc.
Detection Method
Absorbance (450 nm)
Features & Benefits
Convenient & sensitive
The assay can detect as low as 50 uU of NAG activity in a variety of samples
The assay can detect as low as 50 uU of NAG activity in a variety of samples
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for beta-N-Acetylglucosaminidase assay kit
Product Information: beta-N-Acetylglucosaminidase Activity Assay Kit (Colorimetric): Simple & convenient assay to measure NAG activity in biological fluids, tissues & cells. Detection limit: < 50 uU. 100 assay
Background: beta-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and identification of AKI. MyBioSource's NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 uU of NAG activity in a variety of samples.
Summary: Detection method- Absorbance (450 nm)
Application- Measurement of NAG in biological samples from different mammalian species
Background: beta-N-Acetylglucosaminidase (NAG, EC 3.2.1.52) is a lysosomal enzyme that is expressed in various tissues, including kidney, liver and lungs. NAG can cleave N-acetyl-glucosamine, a monosaccharide derivative of glucose. Its concentration in urine is minimal due to its inability to cross the glomerular basal membrane. Increased concentration of NAG in urine indicates renal tubular cell breakdown. Acute Kidney Injury (AKI) is the sudden loss of kidney functions, causing electrolyte imbalance, and retention of urea and other nitrogenous products. NAG has become one of the most studied and used biomarkers for the detection and identification of AKI. MyBioSource's NAG Activity Assay Kit provides a simple and sensitive method for monitoring NAG enzymatic activity. In this assay, NAG uses a synthetic p-nitrophenol derivative (R-pNP) as a NAG substrate and releases pNP which can be measured at absorbance (OD 400 nm). The assay can detect as low as 50 uU of NAG activity in a variety of samples.
Summary: Detection method- Absorbance (450 nm)
Application- Measurement of NAG in biological samples from different mammalian species
Product Categories/Family for beta-N-Acetylglucosaminidase assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
beta-N-acetylglucosaminidase
Protein Family
Similar Products
Product Notes
The beta-N-Acetylglucosaminidase (Catalog #AAA841489) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of NAG in biological samples from different mammalian species. Researchers should empirically determine the suitability of the beta-N-Acetylglucosaminidase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "beta-N-Acetylglucosaminidase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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