Immunohistochemistry (IHC)
(MBS9602194 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse PDE4B/C/D Polyclonal Antibody | anti-PDE4B antibody
PDE4B/C/D Antibody
Gene Names
PDE4B; DPDE4; PDEIVB
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
PDE4B/C/D; Polyclonal Antibody; PDE4B/C/D Antibody; 5''-cyclic phosphodiesterase 4B; cAMP-specific 3''; cAMP-specific 3'; 5'-cyclic phosphodiesterase 4B; cAMP-specific phosphodiesterase-4 B isoform; DKFZp686F2182; DPDE4; dunce-like phosphodiesterase E4; MGC126529; OTTHUMP00000010652; OTTHUMP00000010653; OTTHUMP00000010654; OTTHUMP00000010656; OTTHUMP00000232365; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B_HUMAN; PDE4B5; PDEIVB; Phosphodiesterase 4B; Phosphodiesterase 4B cAMP specific; cAMP specific (phosphodiesterase E4 dunce homolog; Drosophila); 5''-cyclic phosphodiesterase 4C; cAMP specific 3' 5' cyclic phosphodiesterase 4C; DPDE 1; DPDE1; MGC126222; PDE 21; PDE 4C; PDE21; Pde4c; PDE4C_HUMAN; Phosphodiesterase 4C; Phosphodiesterase 4C cAMP specific; cAMP specific (phosphodiesterase E1 dunce homolog; Phosphodiesterase E1 dunce homolog; Phosphodiesterase E1 dunce homolog Drosophila; Phosphodiesterase type 4C; cAMP specific 3'; 5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D; cAMP specific (dunce); STRK1; anti-PDE4B antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
PDE4B/C/D antibody detects endogenous levels of total PDE4B/C/D
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
564
Applicable Applications for anti-PDE4B antibody
Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:1000
IHC: 1:200
IF/ICC: 1:100-1:500
IHC: 1:200
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human PDE4B/C/D
Tissue Specificity
Expressed in brain, heart, lung and skeletal muscle.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9602194 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9602194 at 1/100 staining Human brain cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis of PDE4B/C/D using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis of PDE4B/C/D using HeLa whole cell lysates)
Immunofluorescence (IF)
(MBS9602194 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescence (IF)
(MBS9602194 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-PDE4B antibody
Function: Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
Similarity: Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 76 kDa
Predicted: 84 kDa
Predicted: 84 kDa
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4B isoform 2
NCBI Official Synonym Full Names
phosphodiesterase 4B
NCBI Official Symbol
PDE4B
NCBI Official Synonym Symbols
DPDE4; PDEIVB
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4B
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4B
Protein Family
UniProt Gene Name
PDE4B
UniProt Synonym Gene Names
DPDE4
NCBI Description
This gene is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. The encoded protein regulates the cellular concentrations of cyclic nucleotides and thereby play a role in signal transduction. Altered activity of this protein has been associated with schizophrenia and bipolar affective disorder. Alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2014]
Uniprot Description
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.
Research Articles on PDE4B
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Product Notes
The PDE4B pde4b (Catalog #AAA9602194) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PDE4B/C/D Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PDE4B/C/D can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemisty (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:1000 IHC: 1:200 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the PDE4B pde4b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PDE4B/C/D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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