Typical Testing Data/Standard Curve (for reference only)
Lipolysis (3T3-L1) Assay Kit
Lipolysis (3T3-L1) Colorimetric Assay Kit
Synonyms
Lipolysis (3T3-L1); Lipolysis (3T3-L1) Colorimetric Assay Kit; Lipolysis (3T3-L1) assay kit
Application Notes
Measurement of lipolysis in 3T3-L1 cells or adipocytes
Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Lipolysis (3T3-L1) assay kit
Background/Introduction: Lipolysis is the hydrolysis of triglycerides within the cell into glycerol and free fatty acids. The glycerol and free fatty acids are then released into the bloodstream or culture media. Lipolysis occurs in essentially all cells, but is most abundant in white and brown adipose tissue. Deficiencies in lipolysis lead to increased intracellular lipid accumulation, resulting in abnormal cellular physiology, hyperlipidemia, and insulin resistance. Lipolysis can be induced by catecholamine and certain hormones. The kit includes synthetic catecholamine, Isoproterenol, which activates beta-adrenergic receptors. This leads to activation of adenylate cyclase, which catalyzes the conversion of ATP to cAMP. cAMP then serves as a second messenger to activate hormone-sensitive lipase, which hydrolyzes the triglycerides. This pathway can be inhibited by insulin. 3T3-L1 Lipolysis Assay kit is simple and easy-to-use. The assay measures glycerol released from 3T3-L1 cells after induction of lipolysis using colorimetric method. The color intensity is directly proportional to the amount of glycerol. This assay kit can detect less than 200 pmol of glycerol.
Product Categories/Family for Lipolysis (3T3-L1) assay kit
Similar Products
Product Notes
The Lipolysis (3T3-L1) (Catalog #AAA841692) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of lipolysis in 3T3-L1 cells or adipocytes Screening compounds that influence lipolysis, mechanistic studies, and studies on metabolic dysfunctions. Researchers should empirically determine the suitability of the Lipolysis (3T3-L1) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipolysis (3T3-L1), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
