Typical Testing Data/Standard Curve (for reference only)
Mammalian Lipoprotein Lipase Assay Kit | LPL assay kit
Lipoprotein Lipase Activity Fluorometric Assay Kit
Plasma
Cell lysate and tissue (to determine LPL activity specifically compare results in the presence and absence of LPL inhibitor)
Fast and convenient
Highly Sensitive Fluorometric assay for measuring Lipoprotein Lipase activity in various biological samples
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Background: Lipoprotein lipase (LPL) is a member of the lipase family that hydrolyzes triglycerides in chylomicrons and very low-density lipoprotein (VLDL). Digestion of triglycerides in VLDL by LPL leads to their conversion to intermediate-density lipoprotein (IDL) and then low-density lipoprotein (LDL). LPL is found attached to the luminal surface of endothelial cells in the heart, muscle, and adipose tissue. Mutations in lipoprotein lipase can lead to a variety of disorders such as lipoprotein metabolism, hypertriglyceridemia etc. Overexpression of LPL in mice has been shown to promote obesity and insulin resistance. MyBioSource's LPL Activity Assay Kit contains a quenched substrate that fluoresces upon hydrolysis by LPL. The fluorometric intensity is directly proportional to the amount of substrate hydrolyzed. This assay detects total lipase activity when no inhibitor is used. Comparing results in the presence or absence of an LPL inhibitor allows for quantification of LPL activity specifically. Our results indicate that the majority (~90%) of lipase activity detected by this kit in post-heparin treated mouse plasma is from LPL. To determine the exact LPL specific activity in mouse plasma, measure activity in pre- and post-heparin treated plasma.
Summary: Detection method- Fluorescence (Ex/Em = 482/515 nm)
Species reactivity- Mammalian
NCBI and Uniprot Product Information
NCBI Description
LPL encodes lipoprotein lipase, which is expressed in heart, muscle, and adipose tissue. LPL functions as a homodimer, and has the dual functions of triglyceride hydrolase and ligand/bridging factor for receptor-mediated lipoprotein uptake. Severe mutations that cause LPL deficiency result in type I hyperlipoproteinemia, while less extreme mutations in LPL are linked to many disorders of lipoprotein metabolism. [provided by RefSeq, Jul 2008]
Uniprot Description
LPL: The primary function of this lipase is the hydrolysis of triglycerides of circulating chylomicrons and very low density lipoproteins (VLDL). Binding to heparin sulfate proteogylcans at the cell surface is vital to the function. The apolipoprotein, APOC2, acts as a coactivator of LPL activity in the presence of lipids on the luminal surface of vascular endothelium. Defects in LPL are the cause of lipoprotein lipase deficiency (LPL deficiency); also known as familial chylomicronemia or hyperlipoproteinemia type I. LPL deficiency chylomicronemia is a recessive disorder usually manifesting in childhood. On a normal diet, patients often present with abdominal pain, hepatosplenomegaly, lipemia retinalis, eruptive xanthomata, and massive hypertriglyceridemia, sometimes complicated with acute pancreatitis. Belongs to the AB hydrolase superfamily. Lipase family.
Protein type: Lipid Metabolism - glycerolipid; Phospholipase; Membrane protein, GPI anchor; EC 3.1.1.34
Chromosomal Location of Human Ortholog: 8p22
Cellular Component: extracellular matrix; extracellular space; chylomicron; cell surface; extracellular region; plasma membrane
Molecular Function: heparin binding; triacylglycerol lipase activity; lipoprotein lipase activity; protein binding; apolipoprotein binding; phospholipase activity; receptor binding; triglyceride binding
Biological Process: response to drug; phototransduction, visible light; triacylglycerol metabolic process; phospholipid metabolic process; triacylglycerol catabolic process; triacylglycerol biosynthetic process; lipoprotein metabolic process; response to cold; retinoid metabolic process; fatty acid biosynthetic process
Disease: Hyperlipoproteinemia, Type I; Hyperlipidemia, Familial Combined
Research Articles on LPL
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Product Notes
The Mammalian LPL lpl (Catalog #AAA841897) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA841897 Assay Kit recognizes Mammalian LPL. Measurement of LPL activity in purified wild type or recombinant protein as well as in plasma, cell and tissue lysates. Researchers should empirically determine the suitability of the LPL lpl for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Lipoprotein Lipase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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