Typical Testing Data/Standard Curve (for reference only)
PicoProbe LDH-Cytotoxicity Assay Kit
PicoProbe LDH-Cytotoxicity Fluorometric Assay Kit
Synonyms
PicoProbe LDH-Cytotoxicity; PicoProbe LDH-Cytotoxicity Fluorometric Assay Kit; PicoProbe Lactate Dehydrogenase-Cytotoxicity Fluorometric Assay Kit; PicoProbe LDH-Cytotoxicity assay kit
Application Notes
Measurement of released LDH in culture medium
Evaluation of cytotoxicity effect of test compounds
Evaluation of cytotoxicity effect of test compounds
Samples
Adherent or Suspension Cells
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Highly-Sensitive: Detects as less as 100 cells.
Rapid protocol: requires less than 20 min.
High-throughput adaptable.
Rapid protocol: requires less than 20 min.
High-throughput adaptable.
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe LDH-Cytotoxicity assay kit
Product Information: PicoProbe LDH-Cytotoxicity Fluorometric Assay Kit: Rapid, highly sensitive and high-throughput adaptable assay to quantitate cytotoxicity based on the measurement of activity of Lactate dehydrogenase (LDH) released from damaged cells. Detects as less as ~100 cells. 500 Assays
Background: Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. MyBioSource's PicoProbe LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Applications- Measurement of released LDH in culture medium
Evaluation of cytotoxicity effect of test compounds
Background: Lactate Dehydrogenase (LDH, EC 1.1.1.27) is a stable enzyme and is present in all cell types. Cell damage leads to release of LDH enzyme into the media and its activity is widely used as a marker for cytotoxicity. It reversibly converts lactate into pyruvate, with the concomitant interconversion of NADH and NAD+. MyBioSource's PicoProbe LDH-Cytotoxicity Fluorometric Assay Kit provides a sensitive, quick, and easy way for detection of LDH released from damaged cells. In this assay, LDH converts lactate to pyruvate and NADH, which reduces a proprietary probe to an intensely fluorescent product (Ex/Em = 535/587 nm). The amount of fluorescence is directly proportional to the number of damaged cells. The assay is adaptable to high-throughput format and can be completed in less than 20 min. Sensitivity: ~ 100 cells.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Applications- Measurement of released LDH in culture medium
Evaluation of cytotoxicity effect of test compounds
Product Categories/Family for PicoProbe LDH-Cytotoxicity assay kit
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Product Notes
The PicoProbe LDH-Cytotoxicity (Catalog #AAA841679) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of released LDH in culture medium Evaluation of cytotoxicity effect of test compounds. Researchers should empirically determine the suitability of the PicoProbe LDH-Cytotoxicity for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbe LDH-Cytotoxicity, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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