Typical Testing Data/Standard Curve (for reference only)
PicoProbeTM Dihydroxyacetone Phosphate (DHAP) Assay Kit
PicoProbeTM Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit
Synonyms
PicoProbeTM Dihydroxyacetone Phosphate (DHAP); PicoProbeTM Dihydroxyacetone Phosphate (DHAP) Fluorometric Assay Kit; PicoProbeTM Glycerone Phosphate Colorimetric Assay Kit; PicoProbeTM Dihydroxyacetone Phosphate (DHAP) assay kit
Application Notes
Measurement of Dhydroxyacetone Phosphate in various tissues/cells
Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis
Mechanistic study of human TPI deficiency
Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis
Mechanistic study of human TPI deficiency
Samples
Animal tissues: e.g., liver, muscle etc.
Cell culture: adherent or suspension cells
Biological fluids: serum, plasma etc.
Cell culture: adherent or suspension cells
Biological fluids: serum, plasma etc.
Detection Method
Fluorescence (Ex/Em = 535/587 nm)
Features & Benefits
Simple, Sensitive & Rapid protocol
Highly-Sensitive: Detects as low as 0.5 uM DHAP
Highly-Sensitive: Detects as low as 0.5 uM DHAP
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbeTM Dihydroxyacetone Phosphate (DHAP) assay kit
Product Information: PicoProbe Dihydroxyacetone Phosphate Fluorometric Assay Kit: Only kit on the market to measure DHAP. Rapid, Convenient & Sensitive. Detects as low as 0.5 uM DHAP. 100 Assays.
Background: Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. MyBioSource's PicoProbeTM Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces PicoProbe to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 uM in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application- Measurement of Dhydroxyacetone Phosphate in various tissues/cells
Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis
Mechanistic study of human TPI deficiency
Background: Dihydroxyacetone Phosphate (DHAP) is an important intermediate in both lipid biosynthesis and glycolysis. In glycolysis, Fructose-1,6-diphosphate is converted to DHAP and glyceraldehyde-3-phosphate (GAP) by aldolase. Both DHAP and GAP serve as the intracellular triose phosphate pool. DHAP can be further converted into GAP by Triose Phosphate Isomerase (TPI). In humans, TPI deficiency is a rare autosomal disease. It causes hemolytic anemia, neurological diseases, and even death due to blockage of the glycolytic pathway and accumulation of DHAP in erythrocytes. MyBioSource's PicoProbeTM Dihydroxyacetone Phosphate Assay kit is suitable for measuring low levels of DHAP typically found in a variety of samples. In this kit, TPI converts DHAP to GAP that undergoes a series of reaction and reduces PicoProbe to generate fluorescence. The fluorescence intensity generated is directly proportional to the amount of Dihydroxyacetone Phosphate. This simple, sensitive, and easy to use assay kit can detect Dihydroxyacetone Phosphate as low as 0.5 uM in a variety of samples.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application- Measurement of Dhydroxyacetone Phosphate in various tissues/cells
Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis
Mechanistic study of human TPI deficiency
Product Categories/Family for PicoProbeTM Dihydroxyacetone Phosphate (DHAP) assay kit
Similar Products
Product Notes
The PicoProbeTM Dihydroxyacetone Phosphate (DHAP) (Catalog #AAA841685) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of Dhydroxyacetone Phosphate in various tissues/cells Analysis of metabolic pathways such as glycolysis, Calvin cycle in plants, methylglyoxal pathway and lipid biosynthesis Mechanistic study of human TPI deficiency. Researchers should empirically determine the suitability of the PicoProbeTM Dihydroxyacetone Phosphate (DHAP) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbeTM Dihydroxyacetone Phosphate (DHAP), Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
