Testing Data
Mammalian Phosphatidic Acid Assay Kit
PicoProbe Phosphatidic Acid Assay Kit (Fluorometric)
Reactivity
Mammalian
Synonyms
Phosphatidic Acid; PicoProbe Phosphatidic Acid Assay Kit (Fluorometric); Phosphatidic Acid assay kit
Reactivity
Mammalian
Samples
Cell lipid extract, Tissue lipid extract
Detection Method
Fluorescence: Ex/Em 535/587nm
Features & Benefits
Simple, fast, does not require lenghty incubation times
The easiest, specific assay kit in the market to measure Cardiolipin in biological samples
This assay provides a convenient and non-ELISA alternative to detect phosphatidic acid amount in biological samples.
The easiest, specific assay kit in the market to measure Cardiolipin in biological samples
This assay provides a convenient and non-ELISA alternative to detect phosphatidic acid amount in biological samples.
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Phosphatidic Acid assay kit
Phospholipids are amphiphilic molecules containing phosphate groups, and are both critical components of biological membranes and important signaling messengers in a number of biological pathways. Phosphatidic acid (PA) is the simplest phospholipid, consisting of a glycerol backbone with fatty acids bonded to carbon 1 and 2, and a phosphate group bonded to carbon 3. PA is a precursor for the synthesis of more complex phospholipids such as phosphatidylcholine, phosphatidylethanolamine and phosphatidylserine that act as structural components of the plasma membrane. Phosphatidic acid itself is present in the plasma membrane lending the membrane a high curvature and a net negative charge. In addition, it is an important signaling lipid. It acts through a G-protein coupled receptor and is involved in growth regulation and mitosis. phosphatidic acid assay kit is a plate-based enzymatic assay for quantitation of PA in cells and tissues. The PA Converter hydrolyzes PA to form an intermediate which, in the presence of a developer and enzyme mix, converts a non-fluorescent probe to a fluorescent product (Ex/Em= 535/587 nm) that can be quantified. The Converter is specific to PA and does not hydrolyze other phospholipids (e.g. those with more complex head groups such as phosphatidylcholine or cardiolipin), allowing direct quantitation of PA. This kit can detect as low as 40 pmol PA per well
Product Categories/Family for Phosphatidic Acid assay kit
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Product Notes
The Mammalian Phosphatidic Acid (Catalog #AAA849757) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849757 Assay Kit recognizes Mammalian Phosphatidic Acid. It is sometimes possible for the material contained within the vial of "Phosphatidic Acid, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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