Evaluation of tissue damages or cell cytotoxicity
Cell culture: adherent or suspension Cells
Culture medium & fermentation
Human serum or plasma
High-throughput adaptable
Detection Limit: 1 uU/ml
Shelf Life: 12 months
Background: Lactate Dehydrogenase (LDH: EC 1.1.1.27) is a common enzyme found in wide range of organisms. It catalyzes the interconversion of pyruvate and lactate, with concomitant interconversion of NADH and NAD+. During tissue damage, LDH is released into the bloodstream; therefore it serves as a marker for various diseases and common injuries. MyBioSource's PicoProbe Lactate Dehydrogenase Activity Assay kit provides a quick and easy method for monitoring Lactate Dehydrogenase activity in a wide variety of samples. In this assay, Lactate Dehydrogenase converts lactate into pyruvate and NADH, which reacts with PicoProbe to generate an intense fluorescent product (Ex/Em = 535/587 nm). This kit is simple, highly sensitive and high-throughput adaptable, and can detect Lactate Dehydrogenase activity as low as 1 uU/ml.
Summary: Detection method- Fluorescence (Ex/Em = 535/587 nm)
Application-
Measurement of LDH activity in various tissues and cells
Evaluation of tissue damages or cell cytotoxicity
Similar Products
Product Notes
The PicoProbe Lactate Dehydrogenase Activity Assay Kit (Catalog #AAA841846) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Measurement of LDH activity in various tissues and cells Evaluation of tissue damages or cell cytotoxicity. Researchers should empirically determine the suitability of the PicoProbe Lactate Dehydrogenase Activity Assay Kit for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PicoProbe Lactate Dehydrogenase Activity Assay Kit, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.