Typical Testing Data/Standard Curve (for reference only)
Cholesterol Efflux Assay Kit
Cholesterol Efflux Fluorometric Assay Kit (cell-based)
Synonyms
Cholesterol Efflux; Cholesterol Efflux Fluorometric Assay Kit (cell-based); Cholesterol Efflux assay kit
Application Notes
Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Samples
Serum, Isolated, or recombinant lipoproteins
Detection Method
Fluorescence (Ex/Em = 482/515 nm).
Features & Benefits
Simple & Rapid Protocol
Convenient: Non-Radioactive, no special handling or disposal required
High-Throughput
Accurate: reproducible results with low intra & inter assay variability
Convenient: Non-Radioactive, no special handling or disposal required
High-Throughput
Accurate: reproducible results with low intra & inter assay variability
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for Cholesterol Efflux assay kit
Product Information: Cholesterol Efflux Fluorometric Assay Kit (Cell-Based): Only non-radioactive, high-throughput assay on the market to measure cholesterol efflux or screening small molecules for their effect on cholesterol efflux. 100 Assays
Background: Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. A negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. MyBioSource's Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.
Summary: Detection method- Fluorescence (Ex/Em = 482/515 nm).
Applications-
Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Background: Cholesterol efflux from the peripheral tissues and cells in atherosclerotic plaque is an initial and critical step in Reverse Cholesterol Transport (RCT). RCT is the process by which extrahepatic cells, including macrophage-derived foam cells in arterial atherosclerotic plaque, transport excessive cholesterol back to the liver for bile acid synthesis and excretion, thus lowering the peripheral lipid burden. A negative correlation has been established between the in vitro efflux of cholesterol from macrophages and atherosclerosis. MyBioSource's Cholesterol Efflux Assay is a high-throughput screening assay for measuring cholesterol efflux in cells using fluorescently-labeled cholesterol. This assay provides a safe, sensitive, and reproducible method for measuring cholesterol efflux.
Summary: Detection method- Fluorescence (Ex/Em = 482/515 nm).
Applications-
Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux.
Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program).
Product Categories/Family for Cholesterol Efflux assay kit
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Product Notes
The Cholesterol Efflux (Catalog #AAA841611) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Screen serum samples or lipoproteins (isolated or recombinant) for cholesterol efflux. Screen small molecules for their effect on cholesterol efflux (a valuable tool for drug discovery program). Researchers should empirically determine the suitability of the Cholesterol Efflux for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Cholesterol Efflux, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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