Typical Testing Data/Standard Curve (for reference only)
3T3-L1 assay kit
3T3-L1 Differentiation Kit
Synonyms
3T3-L1; 3T3-L1 Differentiation Kit; 3T3-L1 assay kit
Application Notes
Differentiation of 3T3-L1 preadipocytes to adipocytes
Study of obesity, adipogenesis, lipolysis and lipid metabolism
Study of obesity, adipogenesis, lipolysis and lipid metabolism
Samples
Animal tissues: primary preadipocytes
Cell culture: 3T3-L1 cells
Cell culture: 3T3-L1 cells
Features & Benefits
Simple, rapid & convenient method to differentiate 3T3-L1 preadipocytes to adipocytes
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for 3T3-L1 assay kit
Product Information: 3T3-L1 Differentiation Kit: Simple, rapid & convenient method to differentiate 3T3-L1 preadipocytes to adipocytes. 100 assays.
Background: 3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. MyBioSource's 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 ug/ml insulin, 1 uM dexamethasone, 500 uM IBMX, and 1 uM rosiglitazone in the differentiation media.
Summary: Applications- Differentiation of 3T3-L1 preadipocytes to adipocytes
Study of obesity, adipogenesis, lipolysis and lipid metabolism
Background: 3T3-L1 cells are derived from mouse 3T3 cells and provide a widely-used fundamental model in the study of adipose physiology and metabolic diseases. They exhibit a fibroblast-like morphology before differentiation but become rounded and accumulate lipid droplets several days after the initiation of differentiation. The accumulated lipid droplets can be visualized by light microscopy. The in vitro differentiated 3T3-L1 adipocytes result in characteristics similar to tissue-derived adipocytes and have been commonly used to study adipogenesis, lipolysis, and metabolic dysfunctions. MyBioSource's 3T3-L1 Differentiation Kit provides enough supplements to make 100 ml of differentiation medium and 600 ml of maintenance medium which is sufficient material for 12 100 mm culture dishes. The Differentiation Cocktail provides a final concentration of 1.5 ug/ml insulin, 1 uM dexamethasone, 500 uM IBMX, and 1 uM rosiglitazone in the differentiation media.
Summary: Applications- Differentiation of 3T3-L1 preadipocytes to adipocytes
Study of obesity, adipogenesis, lipolysis and lipid metabolism
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Product Notes
The 3T3-L1 (Catalog #AAA841812) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. Differentiation of 3T3-L1 preadipocytes to adipocytes Study of obesity, adipogenesis, lipolysis and lipid metabolism. Researchers should empirically determine the suitability of the 3T3-L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "3T3-L1, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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