Immunocytochemistry (ICC)
(TEX19.1 in D3 Mouse Cell Line.TEX19.1 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse TEX19.1 Monoclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Rat IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.)
Rat anti-Mouse TEX19.1 Monoclonal Antibody | anti-Tex19.1 antibody
TEX19.1 (Testis-expressed Protein 19.1, Testis-expressed Protein 19A, Testis-expressed Sequence 19A, Tex19, Tex19a)
Gene Names
Tex19.1; Tex19; Tex19a; 2410081M02Rik
Reactivity
Mouse
Applications
Immunocytochemistry
Purity
Affinity PurifiedPurified by Protein A/G affinity chromatography.
Synonyms
TEX19.1; Monoclonal Antibody; TEX19.1 (Testis-expressed Protein 19.1; Testis-expressed Protein 19A; Testis-expressed Sequence 19A; Tex19; Tex19a); Anti -TEX19.1 (Testis-expressed Protein 19.1; anti-Tex19.1 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
11n188
Specificity
Recognizes mouse TEX19.1. No cross-reactivity with recombinant human TEX19, recombinant mouse TEX19.2, or rmTEX11.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Applicable Applications for anti-Tex19.1 antibody
Immunocytochemistry (ICC)
Application Notes
Suitable for use in Immunocytochemistry.
Dilution: Immunocytochemistry: 8-25ug/ml
Dilution: Immunocytochemistry: 8-25ug/ml
Immunogen
Recombinant corresponding to aa189-351 from mouse TEX19.1 expressed in E. coli (Q99MV2).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunocytochemistry (ICC)
(TEX19.1 in D3 Mouse Cell Line.TEX19.1 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse TEX19.1 Monoclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Rat IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.)
Immunocytochemistry (ICC)
(TEX19.1 in D3 Mouse Cell Line.TEX19.1 was detected in immersion fixed D3 mouse embryonic stem cell line using Mouse TEX19.1 Monoclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Rat IgG Secondary Antibody and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.)
Related Product Information for anti-Tex19.1 antibody
Testis-expressed protein 19.1 is a 40kD pluripotent stem cell molecule. Mouse TEX19.1 is 351aa in length. The first conserved domain is localized on the N-terminal boundary of the protein and is 58 residues long. It is known as the MCP domain, and begins with an invariant MCPPVS motif. The second conserved domain, known as the VPTEL domain, begins with an invariant VPTEL motif and is 38aa long. Mouse TEX19.1 shares 70-24aa identity with rat and human Tex19.1, respectively. The protein is restricted to mammals and is expressed in testis, placenta, and ovary. It is expressed in early embryo and is later limited to the germ line. Tex19.1 has been shown to present in the cytoplasm of spermatogonia and early spermatocytes in adult mouse testes (1). The precise function of TEX19.1 is still unknown, but it is thought to be a new transcription factor regulating target genes responsible for pluripotency or it has a role in controlling the chromatin architecture of the pluripotent nucleus.
Product Categories/Family for anti-Tex19.1 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,401 Da
NCBI Official Full Name
testis-expressed protein 19.1
NCBI Official Synonym Full Names
testis expressed gene 19.1
NCBI Official Symbol
Tex19.1
NCBI Official Synonym Symbols
Tex19; Tex19a; 2410081M02Rik
NCBI Protein Information
testis-expressed protein 19.1; testis expressed gene 19; testis-expressed protein 19A; testis-expressed sequence 19A
UniProt Protein Name
Testis-expressed protein 19.1
Protein Family
UniProt Gene Name
Tex19.1
UniProt Synonym Gene Names
Tex19; Tex19a
UniProt Entry Name
TX19A_MOUSE
Uniprot Description
Subcellular location: Nucleus Ref.4.
Tissue specificity: Expressed in testis, placenta and ovary. Expressed in pluripotent stem cells. Ref.4
Developmental stage: Expressed in early embryo and is later limited to the germ line. Ref.4
Research Articles on Tex19.1
Similar Products
Product Notes
The Tex19.1 tex19.1 (Catalog #AAA647409) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The TEX19.1 (Testis-expressed Protein 19.1, Testis-expressed Protein 19A, Testis-expressed Sequence 19A, Tex19, Tex19a) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TEX19.1 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC). Suitable for use in Immunocytochemistry. Dilution: Immunocytochemistry: 8-25ug/ml. Researchers should empirically determine the suitability of the Tex19.1 tex19.1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TEX19.1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
