Mouse anti-Human EOMES Monoclonal Antibody | anti-EOMES antibody

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, T-brain-2, TBR-2, TBR2)

Cat. Num. AAA6010440

• Gene Names: EOMES; TBR2
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunocytochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: EOMES; Monoclonal Antibody; EOMES (Eomesodermin Homolog; T-box Brain Protein 2; T-brain-2; TBR-2; TBR2); Anti -EOMES (Eomesodermin Homolog; anti-EOMES antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 11n64
• Specificity: Recognizes human EOMES. No cross-reactivity with recombinant human Brachyury, rhEOMES (aa1-115), recombinant mouse EOMES (aa1-126), rhTBX2, 3, 5, 6, 18, or 20.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, Trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-EOMES antibody: ELISA (EL/EIA), Western Blot (WB), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in ELISA, Western Blot, Flow Cytometry and Immunocytochemistry.; Dilution: Western Blot: 1ug/ml; Immunocytochemistry: 8-25ug/ml; Flow Cytometry: 2.5ug/106 cells
• Immunogen: Recombinant corresponding to aa471-686 from human EOMES expressed in E. coli (O95936).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Testing Data

(EOMES in Mesodermally Differentiated BG01V Human Stem Cells. EOMES was detected in immersion fixed BG01V human embryonic stem cells differentiated into mesoderm using MBS6010440 at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557­conjugated Mouse IgG Secondary Antibody and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei.)

Related Product Information for anti-EOMES antibody

EOMES is a 72kD member of the TBR1 subfamily, T-box family of transcription factors. It is expressed in NK and CD8+ T cells, where CTLA4 activation suppresses EOMES activation of IFN-g and granzyme B genes. It is also found in the embryo, where it occurs in forebrain floorplate and migrating neuroblasts at 12.5 weeks gestation. Notably, it is reported to undergo intercellular transfer in fetal Xenopus tissue destined to become mesoderm. Here, it synchronizes a multicellular commitment to a cell lineage.

Product Categories/Family for anti-EOMES antibody

Antibodies; Abs to Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 22538470
• NCBI GeneID: 8320
• NCBI Accession #: NP_005433.2
• NCBI GenBank Nucleotide #: NM_005442.3
• UniProt Accession #: O95936; Q8TAZ2; Q9UPM7; B7Z4I2
• Molecular Weight: 72,732 Da
• NCBI Official Full Name: eomesodermin homolog isoform 2
• NCBI Official Synonym Full Names: eomesodermin
• NCBI Official Symbol: EOMES
• NCBI Official Synonym Symbols: TBR2
• NCBI Protein Information: eomesodermin homolog; T-box brain protein 2
• UniProt Protein Name: Eomesodermin homolog
• Protein Family: Eomesodermin
• UniProt Gene Name: EOMES
• UniProt Synonym Gene Names: TBR2; T-brain-2; TBR-2
• UniProt Entry Name: EOMES_HUMAN

NCBI Description

This gene belongs to the TBR1 (T-box brain protein 1) sub-family of T-box genes that share the common DNA-binding T-box domain. The encoded protein is a transcription factor which is crucial for embryonic development of mesoderm and the central nervous system in vertebrates. The protein may also be necessary for the differentiation of effector CD8+ T cells which are involved in defense against viral infections. A similar gene disrupted in mice is shown to be essential during trophoblast development and gastrulation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

EOMES: Functions as a transcriptional activator playing a crucial role during development. Functions in trophoblast differentiation and later in gastrulation, regulating both mesoderm delamination and endoderm specification. Plays a role in brain development being required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex. Also involved in the differentiation of CD8+ T-cells during immune response regulating the expression of lytic effector genes. Up-regulated in CD8+ T-cells simultaneously stimulated with TGFB1 and IL4/interleukin-4. Expressed in CD8+ T-cells. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 3p24.1

Cellular Component: nucleus

Molecular Function: DNA binding; sequence-specific DNA binding; chromatin binding; transcription factor activity

Biological Process: transcription, DNA-dependent; CD8-positive, alpha-beta T cell differentiation during immune response; regulation of neuron differentiation; positive regulation of transcription, DNA-dependent; endodermal cell fate specification; olfactory bulb development; stem cell maintenance; negative regulation of transcription from RNA polymerase II promoter; cerebral cortex regionalization; trophectodermal cell differentiation; cerebral cortex neuron differentiation; cardioblast differentiation; mesoderm formation; endoderm formation; interferon-gamma production; positive regulation of transcription from RNA polymerase II promoter; brain development; positive regulation of cell differentiation