Mouse anti-Human SULT4A1 Monoclonal Antibody | anti-SULT4A1 antibody

SULT4A1 (sulfotransferase family 4A, Member 1, Brain sulfotransferase-like protein, Nervous system sulfotransferase, brain sulphotransferase-like, nervous system cytosolic sulfotransferase, sulfotransferase-related protein)

Cat. Num. AAA647252

• Gene Names: SULT4A1; NST; BRSTL1; SULTX3; BR-STL-1; DJ388M5.3; hBR-STL-1
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: SULT4A1; Monoclonal Antibody; SULT4A1 (sulfotransferase family 4A; Member 1; Brain sulfotransferase-like protein; Nervous system sulfotransferase; brain sulphotransferase-like; nervous system cytosolic sulfotransferase; sulfotransferase-related protein); Anti -SULT4A1 (sulfotransferase family 4A; anti-SULT4A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 11n178
• Specificity: Recognizes human SULT4A1. No cross-reactivity with recombinant human SULT1A1, rhSULT1A3, rhSULT1B1, rhSULT1E1, rhSULT2A1, or rhSULT2B1.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-SULT4A1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot and Immunohistochemistry.; Dilution: Western Blot: 2ug/ml; Immunohistochemistry: 8-25ug/ml
• Immunogen: Recombinant corresponding to aa2-284 from Cytosolic Sulfotransferase 4A1/SULT4A1 expressed in E. coli (Q9BR01).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human Cytosolic Sulfotransferase 4A1/SULT4A1 by Western Blot. Western blot shows lysates of human brain (cortex) tissue. PVDF Membrane was probed with 2ug/ml of Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody followed by HRP­conjugated Mouse IgG Secondary Antibody. A specific band was detected for Cytosolic Sulfotransferase 4A1/SULT4A1 at approximately 35kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Immunohistochemistry (IHC)

(Cytosolic Sulfotransferase 4A1/SULT4A1 in Human Brain. Cytosolic Sulfotransferase 4A1/SULT4A1 was detected in immersion fixed paraffin­ embedded sections of human brain (cortex) using Human Cytosolic Sulfotransferase 4A1/SULT4A1 Monoclonal Antibody at 15ug/ml overnight at 4 ° C. Before incubation with the primary antibody, tissue was subjected to heat­ induced epitope retrieval using Antigen Retrieval Reagent­Basic. Tissue was stained using the Mouse HRP­DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). View our protocol f o r Chromogenic IHC Staining of Paraffin­embedded Tissue Sections.)

Related Product Information for anti-SULT4A1 antibody

Cytosolic SulT4A1 is a 33-36kD member of the sulfotransferase 4 family of enzymes. SulT4A1 message is widely expressed. However, it appears to be translated only in brain. SulT4A is very unusual in that it is the only SulT that shows minimal activity on synthetic laboratory substrates. Instead, it acts on catecholamines and T4 in a manner that may not involve sulfonation. Human SulT4A1 is 284aa in length. A weak PAPS binding site is found between aa246-254. There are two potential isoforms, one that shows a 13aa substitution for aa248-284, and a second that contains a 48aa substitution for aa202-247. Full length human SulT4A1 is 98aa identical to mouse SulT4A1.

Product Categories/Family for anti-SULT4A1 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 7657633
• NCBI GeneID: 25830
• NCBI Accession #: NP_055166.1
• NCBI GenBank Nucleotide #: NM_014351.3
• UniProt Accession #: Q9BR01; O43728; B2R7N3
• Molecular Weight: 33,085 Da
• NCBI Official Full Name: sulfotransferase 4A1
• NCBI Official Synonym Full Names: sulfotransferase family 4A, member 1
• NCBI Official Symbol: SULT4A1
• NCBI Official Synonym Symbols: NST; BRSTL1; SULTX3; BR-STL-1; DJ388M5.3; hBR-STL-1
• NCBI Protein Information: sulfotransferase 4A1; ST4A1; hBR-STL; nervous system sulfotransferase; sulfotransferase-related protein; brain sulfotransferase-like protein; nervous system cytosolic sulfotransferase
• UniProt Protein Name: Sulfotransferase 4A1
• Protein Family: Sulfotransferase
• UniProt Gene Name: SULT4A1
• UniProt Synonym Gene Names: SULTX3; ST4A1; hBR-STL; hBR-STL-1; NST
• UniProt Entry Name: ST4A1_HUMAN

NCBI Description

This gene encodes a member of the sulfotransferase family. The encoded protein is a brain-specific sulfotransferase believed to be involved in the metabolism of neurotransmitters. Polymorphisms in this gene may be associated with susceptibility to schizophrenia. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Atypical sulfotransferase family member with very low affinity for 3'-phospho-5'-adenylyl sulfate (PAPS) and very low catalytic activity towards L-triiodothyronine, thyroxine, estrone, p-nitrophenol, 2-naphthylamine, and 2-beta-naphthol. May have a role in the metabolism of drugs and neurotransmitters in the CNS. Ref.12

Subcellular location: Cytoplasm

By similarity.

Tissue specificity: Highly expressed in the cerebral cortex and frontal lobe, slightly less in the cerebellum, occipital and temporal lobes, relatively low in the medulla and putamen, and lowest in the spinal cord. No expression detected in the pancreas (Ref.1). Highly expressed in fetal brain and occipital lobe, slightly less in the whole brain, frontal lobe, hippocampus, and lung, very low expression in cerebellum, medulla oblongata, temporal lobe, testis, kidney and appendix (Ref.2).

Sequence similarities: Belongs to the sulfotransferase 1 family.