Sheep anti-Mouse HS6ST3 Polyclonal Antibody | anti-HS6ST3 antibody

HS6ST3 (Heparan sulfate 6-O-sulfotransferase 3)

Cat. Num. AAA641187

• Gene Names: HS6ST3; HS6ST-3
• Reactivity: Mouse
• Applications: Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HS6ST3; Polyclonal Antibody; HS6ST3 (Heparan sulfate 6-O-sulfotransferase 3); Anti -HS6ST3 (Heparan sulfate 6-O-sulfotransferase 3); anti-HS6ST3 antibody

• Host: Sheep
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse HS6ST3.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.

• Applicable Applications for anti-HS6ST3 antibody: Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Immunohistochemistry.; Dilution: Immunohistochemistry: 5-15ug/ml
• Immunogen: Recombinant corresponding to aa28-470 from mouse HS6ST3 expressed in CHO cells (NP_056635).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Immunohistochemistry (IHC)

(Heparan Sulfate 6­O­ Sulfotransferase 3/HS6ST3 in Mouse Brain . Heparan Sulfate 6­O­ Sulfotransferase 3/HS6ST3 was detected in perfusion fixed frozen sections of mouse brain (trigeminal ganglion) using Sheep Mouse Heparan Sulfate 6­O­ Sulfotransferase 3/HS6ST3 Antigen Affinity­ purified Polyclonal Antibody at 5ug/ml overnight at 4 °C. Tissue was stained using the Sheep HRP­DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)

Related Product Information for anti-HS6ST3 antibody

HS6ST3 (Heparin sulfate 6-O-sulfotransferase 3) is a 60-62kD member of the sulfotransferase 6 family of enzymes. It is one of three 6-O sulfotransferase isoforms, all of which essentially perform the same function, but all of which are the products of distinct genes. HS6ST3 is known to sulfate the #6 position of glucosamine sulfate that is linked to a 2-O-sulfated iduronic acid in heparin sulfate. The molecule shows a temporal expression pattern, being found in postnatal cerebellar Purkinje cells and cerebral cortical pyramidal cells in layers II/III and V. Mouse HS6ST3 is a 470aa type II transmembrane glycoprotein. It contains a short four aa cytoplasmic N-terminus coupled to an extended 443aa lumenal domain (aa28-470). The lumenal domain contains a stem region (aa28-83) that mediates oligomerization and Golgi localization, plus a sulfotransferase enzyme domain (aa142-409). Cleavage within the stem region by b-secretase generates a soluble form of HS6ST3. Over aa28-470, mouse HS6ST3 shares 99-95aa identity with rat and human HS6ST3, respectively.

Product Categories/Family for anti-HS6ST3 antibody

Antibodies; Abs to Enzymes; Sulfotransferase

NCBI and Uniprot Product Information

• NCBI GI #: 45580707
• NCBI GeneID: 266722
• NCBI Accession #: NP_703157.2
• NCBI GenBank Nucleotide #: NM_153456.3
• UniProt Accession #: Q8IZP7; Q5W0L0; Q68CW6
• Molecular Weight: 54,844 Da
• NCBI Official Full Name: heparan-sulfate 6-O-sulfotransferase 3
• NCBI Official Synonym Full Names: heparan sulfate 6-O-sulfotransferase 3
• NCBI Official Symbol: HS6ST3
• NCBI Official Synonym Symbols: HS6ST-3
• NCBI Protein Information: heparan-sulfate 6-O-sulfotransferase 3
• UniProt Protein Name: Heparan-sulfate 6-O-sulfotransferase 3
• Protein Family: Heparan-sulfate 6-O-sulfotransferase
• UniProt Gene Name: HS6ST3
• UniProt Synonym Gene Names: HS6ST-3
• UniProt Entry Name: H6ST3_HUMAN

NCBI Description

Heparan sulfate (HS) sulfotransferases, such as HS6ST3, modify HS to generate structures required for interactions between HS and a variety of proteins. These interactions are implicated in proliferation and differentiation, adhesion, migration, inflammation, blood coagulation, and other diverse processes (Habuchi et al., 2000 [PubMed 10644753]).[supplied by OMIM, Mar 2008]

Uniprot Description

HS6ST3: 6-O-sulfation enzyme which catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to position 6 of the N-sulfoglucosamine residue (GlcNS) of heparan sulfate. Belongs to the sulfotransferase 6 family.

Protein type: Glycan Metabolism - heparan sulfate biosynthesis; Transferase; EC 2.8.2.-; Membrane protein, integral

Chromosomal Location of Human Ortholog: 13q32.1

Cellular Component: integral to membrane

Molecular Function: heparan sulfate 6-O-sulfotransferase activity

Biological Process: heparan sulfate proteoglycan biosynthetic process, enzymatic modification