Western Blot (WB)
(Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line, Nalm6 human PreB acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1ug/ml of Sheep Human Acetyl Histone H4 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Acetyl Histone H4 at approximately 12kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Sheep anti-Human Histone H4, acetylated Polyclonal Antibody
Histone H4, acetylated (Histone Cluster 4 H4, H4/p, HIST4H4, MGC24116)
Reactivity
Human
Applications
Western Blot, Immunocytochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Histone H4; acetylated; Polyclonal Antibody; acetylated (Histone Cluster 4 H4; H4/p; HIST4H4; MGC24116); Anti -Histone H4; anti-Histone H4; acetylated antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes Acetyl-Histone H4.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Applicable Applications for anti-Histone H4, acetylated antibody
Western Blot (WB), Immunocytochemistry (ICC)
Application Notes
Suitable for use in Western Blot and Immunocytochemistry.
Dilution: Western Blot: 1ug/ml
Immunocytochemistry: 5-15ug/ml
Dilution: Western Blot: 1ug/ml
Immunocytochemistry: 5-15ug/ml
Immunogen
Synthetic peptide corresponding to AGGKGGKGMGKVGAKRHSK Tetrahymena pyriformis acetylated Histone H4 (P02310).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line, Nalm6 human PreB acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1ug/ml of Sheep Human Acetyl Histone H4 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Acetyl Histone H4 at approximately 12kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Western Blot (WB)
(Detection of Human Acetyl Histone H4 by Western Blot. Western blot shows lysates of Tera2 human embryonic lung carcinoma cell line, Nalm6 human PreB acute lymphocytic leukemia cell line, and Jurkat human acute T cell leukemia cell line. PVDF Membrane was probed with 1ug/ml of Sheep Human Acetyl Histone H4 Antigen Affinitypurified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Acetyl Histone H4 at approximately 12kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Immunocytochemistry (ICC)
(Histone H4 in NTera2 Human Cell Line. Histone H4 was detected in immersion fixed NTera2 human testicular embryonic carcinoma cell line using Sheep Human Acetyl Histone H4 Antigen Affinity purified Polyclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Sheep IgG Secondary Antibody and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.)
Immunocytochemistry (ICC)
(Histone H4 in NTera2 Human Cell Line. Histone H4 was detected in immersion fixed NTera2 human testicular embryonic carcinoma cell line using Sheep Human Acetyl Histone H4 Antigen Affinity purified Polyclonal Antibody at 10ug/ml for 3 hours at room temperature. Cells were stained using the NorthernLightsTM 557conjugated Sheep IgG Secondary Antibody and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.)
Related Product Information for anti-Histone H4, acetylated antibody
Histone 4 is a 12-15kD member of the histone H4 family of molecules. It is ubiquitously expressed, with H4 initially synthesized and deposited in replicating chromatin during S phase. H4 undergoes multiple posttranslational modifications. At the G2/M transition, H4 is monomethylated on Lys20 and possibly contributes to transcription initiation/elongation. As the cell moves into the M/G1 phase, Lys20 becomes either dimethylated or trimethylated, contributing to DNA repair and telomere maintenance, respectively. H4 is also known to be phosphorylated on aa2-48, plus aa52-89, and acetylated on at least nine sites. Human histone 4 is 103aa in length. Full length histone 4 is identical in aa sequence to mouse histone 4, and 78aa identical to Tetrahymena histone 4 over aa2-20 used for immunization.
Product Categories/Family for anti-Histone H4, acetylated antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
histone H4 - starfish (Asterina pectinifera)
Similar Products
Product Notes
The Histone H4, acetylated (Catalog #AAA642234) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Histone H4, acetylated (Histone Cluster 4 H4, H4/p, HIST4H4, MGC24116) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Histone H4, acetylated can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC). Suitable for use in Western Blot and Immunocytochemistry. Dilution: Western Blot: 1ug/ml Immunocytochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the Histone H4, acetylated for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Histone H4, acetylated, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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