Western Blot (WB)
(Detection of Mouse and Rat Brorin/VWC2 by Western Blot. Western blot shows lysates of rat cortical neuron tissue and mouse brain (thalamus/hypothalamus) tissue. PVDF Membrane was probed with 1ug/ml of Sheep Mouse Brorin/VWC2 Antigen Affinity purified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Brorin/VWC2 at approximately 35kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Sheep anti-Mouse VWC2 Polyclonal Antibody | anti-Vwc2 antibody
VWC2 (Von Willebrand Factor C Domain Containing 2 Brain-specific Chordin-like Protein, Brorin, UNQ739/PRO1434)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Western Blot (WB)
(Detection of Mouse and Rat Brorin/VWC2 by Western Blot. Western blot shows lysates of rat cortical neuron tissue and mouse brain (thalamus/hypothalamus) tissue. PVDF Membrane was probed with 1ug/ml of Sheep Mouse Brorin/VWC2 Antigen Affinity purified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Brorin/VWC2 at approximately 35kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Mouse and Rat Brorin/VWC2 by Western Blot. Western blot shows lysates of rat cortical neuron tissue and mouse brain (thalamus/hypothalamus) tissue. PVDF Membrane was probed with 1ug/ml of Sheep Mouse Brorin/VWC2 Antigen Affinity purified Polyclonal Antibody followed by HRP conjugated Sheep IgG Secondary Antibody. A specific band was detected for Brorin/VWC2 at approximately 35kD (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Immunohistochemistry (IHC)
(Brorin/VWC2 in Mouse Embryo. Brorin/VWC2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Mouse Brorin/VWC2 Antigen Affinitypurified Polyclonal Antibody at 5ug/ml overnight at 4 °C. Tissue was stained using the Sheep HRPDAB Cell & Tissue Staining Kit a n d counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of mesenchyme/muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
Immunohistochemistry (IHC)
(Brorin/VWC2 in Mouse Embryo. Brorin/VWC2 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Mouse Brorin/VWC2 Antigen Affinitypurified Polyclonal Antibody at 5ug/ml overnight at 4 °C. Tissue was stained using the Sheep HRPDAB Cell & Tissue Staining Kit a n d counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of mesenchyme/muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
NCBI and Uniprot Product Information
Uniprot Description
Function: BMP antagonist which may play a role in neural development. Promotes cell adhesion. Ref.1 Ref.7
Subunit structure: Peripherally associated with AMPAR complex. AMPAR complex consists of an inner core made of 4 pore-forming GluA/GRIA proteins (GRIA1, GRIA2, GRIA3 and GRIA4) and 4 major auxiliary subunits arranged in a twofold symmetry. One of the two pairs of distinct binding sites is occupied either by CNIH2, CNIH3 or CACNG2, CACNG3. The other harbors CACNG2, CACNG3, CACNG4, CACNG8 or GSG1L. This inner core of AMPAR complex is complemented by outer core constituents binding directly to the GluA/GRIA proteins at sites distinct from the interaction sites of the inner core constituents. Outer core constituents include at least PRRT1, PRRT2, CKAMP44/SHISA9, FRRS1L and NRN1. The proteins of the inner and outer core serve as a platform for other, more peripherally associated AMPAR constituents, including VWC2. Alone or in combination, these auxiliary subunits control the gating and pharmacology of the AMPAR complex and profoundly impact their biogenesis and protein processing. Ref.8
Subcellular location: Secreted › extracellular space › extracellular matrix › basement membrane. Cell junction › synapse
Probable Ref.1 Ref.7 Ref.8.
Tissue specificity: Predominantly expressed in the brain (at protein level). It is expressed in the neurons but not the glial cells. Ref.1 Ref.7 Ref.8
Developmental stage: At 12.5 dpc, predominantly expressed in the developing diencephalon. At 16.5 dpc and 18.5 dpc, expressed in the brain, spinal cord, developing neural tubes and tongue but not in the cerebral cortex. At 16.5 dpc, present in developing oral and tooth germ epithelia (at protein level). Ref.1 Ref.7
Sequence similarities: Contains 2 VWFC domains.
Sequence caution: The sequence CAI25053.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.
Similar Products
Product Notes
The Vwc2 vwc2 (Catalog #AAA647188) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The VWC2 (Von Willebrand Factor C Domain Containing 2 Brain-specific Chordin-like Protein, Brorin, UNQ739/PRO1434) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VWC2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the Vwc2 vwc2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VWC2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
