Immunohistochemistry (IHC)
(TCL1B in Human Lymphoma. TCL1B was detected in immersion fixed paraffin embedded sections of human lymphoma using Goat Human TCL1B Antigen Affinity purified Polyclonal Antibody at 3ug/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heatinduced epitope retrieval using Antigen Retrieval Reagent Basic. Tissue was stained using the Goat HRPDAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin embedded Tissue Sections.)
Goat anti-Human TCL1B Polyclonal Antibody | anti-TCL1B antibody
TCL1B (T cell leukemia protein 1B, SYN1, [p15]TML1)
Gene Names
TCL1B; TML1; SYN-1
Reactivity
Human
Applications
Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
TCL1B; Polyclonal Antibody; TCL1B (T cell leukemia protein 1B; SYN1; [p15]TML1); Anti -TCL1B (T cell leukemia protein 1B; anti-TCL1B antibody
Host
Goat
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human TCL1B. Species sequence homology: recombinant human TCL1A (5%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 200ug/ml.
Applicable Applications for anti-TCL1B antibody
Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry.
Dilution: Immunohistochemistry: 5-15ug/ml
Dilution: Immunohistochemistry: 5-15ug/ml
Immunogen
Recombinant corresponding to aa2-128 (aa93) from human TCL1B expressed in E. coli (O95988).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(TCL1B in Human Lymphoma. TCL1B was detected in immersion fixed paraffin embedded sections of human lymphoma using Goat Human TCL1B Antigen Affinity purified Polyclonal Antibody at 3ug/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heatinduced epitope retrieval using Antigen Retrieval Reagent Basic. Tissue was stained using the Goat HRPDAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin embedded Tissue Sections.)
Immunohistochemistry (IHC)
(TCL1B in Human Lymphoma. TCL1B was detected in immersion fixed paraffin embedded sections of human lymphoma using Goat Human TCL1B Antigen Affinity purified Polyclonal Antibody at 3ug/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heatinduced epitope retrieval using Antigen Retrieval Reagent Basic. Tissue was stained using the Goat HRPDAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin embedded Tissue Sections.)
Related Product Information for anti-TCL1B antibody
TCL1B is a monomeric,15kD (predicted) member of the TCL1 family of proteins. It is expressed in the embryo and syncytiotrophoblast, and interacts with Akt1 and Akt2 kinase, promoting their activation and the subsequent deactivation of proapoptotic molecules. Human TCL1B is 128aa in length. There is no definitive structural motif that characterizes TCL1B, save for a unique b-barrel configuration. Full length human TCL1B shares less than 35aa identity with five orthologous mouse TCL1B isoforms.
Product Categories/Family for anti-TCL1B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
14,846 Da
NCBI Official Full Name
T-cell leukemia/lymphoma protein 1B
NCBI Official Synonym Full Names
T-cell leukemia/lymphoma 1B
NCBI Official Symbol
TCL1B
NCBI Official Synonym Symbols
TML1; SYN-1
NCBI Protein Information
T-cell leukemia/lymphoma protein 1B; oncogene TCL-1B; TCL1/ MTCP1-like 1; T-cell lymphoma/leukemia 1B; syncytiotrophoblast-specific protein
UniProt Protein Name
T-cell leukemia/lymphoma protein 1B
Protein Family
UniProt Gene Name
TCL1B
UniProt Synonym Gene Names
TCL1; Oncogene TCL1B
UniProt Entry Name
TCL1B_HUMAN
Uniprot Description
Function: Enhances the phosphorylation and activation of AKT1 and AKT2. Ref.8
Subunit structure: Interacts with AKT1 and AKT2 (via PH domain). Does not interact with AKT3. Ref.8 Ref.9
Tissue specificity: Expressed in a variety of tissues including placenta and testis. Ref.1 Ref.2
Miscellaneous: Activated in chronic T-cell leukemias (T-CLL) carrying rearrangements of the 14q32.1 region.
Sequence similarities: Belongs to the TCL1 family.
Research Articles on TCL1B
Similar Products
Product Notes
The TCL1B tcl1b (Catalog #AAA645823) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The TCL1B (T cell leukemia protein 1B, SYN1, [p15]TML1) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's TCL1B can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry. Dilution: Immunohistochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the TCL1B tcl1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TCL1B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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