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Type
Reactivity
Gene Name
Isotype
Host
Application
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1983 results for "FC Blocking" - showing 50-100
Western Blot
( All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Prohibitin (PHB1), Polyclonal Antibody (Cat# AAA9202298)
Full Name
Prohibitin (PHB1) Antibody (N-term)
Gene Names
PHB; PHB1; HEL-215; HEL-S-54e
Reactivity
Human, Mouse
Predicted Reactivity: Bovine, Chicken
Predicted Reactivity: Bovine, Chicken
Applications
FC/FACS, EIA, WB, IHC-P
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Testing Data
(Published customer image: NKG2D-dependent infiltration of CD8+T cells into tumors. Tumors were collected from mice that had received one of the four standard treatments: control DNA, Dox plus control DNA, IL-12, Dox plus IL-12 (n = 3 per treatment group). (A) Infiltration of NKG2D-positive cells into tumors. Northern blot analysis was performed to detect NKG2D expression in tumors. Ribosomal RNA was used to confirm equal loading among samples. (B) NKG2D/CD8 -positive cells in tumor sections by treatment received. Frozen tumor sections were stained with biotin anti-mouse NKG2D, anti-mouse CD8, or corresponding isotype control antibodies, then with streptavidin-conjugated Alexa fluor 594 or Alexa fluor 488 secondary antibodies. Data shown are representative of three independent experiments. The scale bar is equivalent to 100 um.From: CD8+T cell-specific induction of NKG2D receptor by doxorubicin plus interleukin-12 and its contribution to CD8+T cell accumulation in tumors. Hu J, Zhu S, Xia X, Zhang L, Kleinerman ES, Li S. Mol Cancer. 2014 Feb 24;13:34.)
CD8 ALPHA, Monoclonal Antibody (Cat# AAA214268)
Full Name
RAT ANTI MOUSE CD8:Low Endotoxin
Gene Names
Cd8a; Ly-2; Ly-B; Ly-35; Lyt-2; BB154331
Applications
FC/FACS, IF
Pricing
$745/0.5 mg | $3,200/5x0.5 mg
Immunoprecipitation (IP)
(PRKDC was immunoprecipitated from 1mg of HepG2 cells membrane fraction, blotted with MBS435247 of 10 ug. Western blot was performed from the immunopre-cipitate using MBS435247 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: HepG2 cells membrane fraction. Lane2: IP product of HepG2 cells membrane fraction.Predicted band size : 469 KDaObserved band size : 469 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
DNA PKcs, Monoclonal Antibody (Cat# AAA435247)
Full Name
DNA PKcs Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded Human Heart stained with Cytochrome C Monoclonal Antibody (CTC05).)
Cytochrome C, Monoclonal Antibody (Cat# AAA438650)
Full Name
Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat, Horse, Dog, Pigeon, Frog, Drosophila.
Others not known
Others not known
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot using anti-TIGIT antibody 1B4 RAW-264.7 cells (A), mouse spleen (B) and mouse thymus (C) lysates (35ug protein in RIPA buffer) were resolved on a 10% SDS PAGE gel and blots probed with the chimeric rabbit IgG version of 1B4 (MBS488486) at 0.3 ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
TIGIT, Monoclonal Recombinant Antibody (Cat# AAA488486)
Full Name
Anti-TIGIT [1B4]
Gene Names
Tigit; Vstm3
Reactivity
Mouse
Applications
EIA, FC/FACS, BL
Purity
Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Immunohistochemistry (IHC)
(Immunohistochemical analysis of MBS9203877 on paraffin-embedded human kidney tissue was performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:250) for 15min at room temperature. Leica Bond Polymer Refine Detection was used as the secondary antibody.)
NEU2, Polyclonal Antibody (Cat# AAA9203877)
Full Name
NEU2 Antibody (N-term)
Gene Names
NEU2; SIAL2
Reactivity
Human
Applications
WB, EIA, IHC-P-Leica, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
CD335, Monoclonal Antibody (Cat# AAA211240)
Full Name
MOUSE ANTI BOVINE CD335:Low Endotoxin
Gene Names
NCR1; NKP46
Applications
FC/FACS, FN, IF, WB
Pricing
$755/0.5 mg | $3,255/5x0.5 mg
CCR7, Polyclonal Antibody (Cat# AAA9205978)
Full Name
CCR7 Antibody (N-term)
Gene Names
CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
Reactivity
Human, mouse
Applications
WB, IHC-P, IF , FC, E
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(All lanes : LMNA Mouse mAb at 1/2000 dilution Lane 1 : THP1 cells membrane fraction Lane 2 : Jurkat cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 65 KDa Observed band size : 65 KDa Blocking/Dilution buffer : 1x TBST.)
Lamin A/C, Monoclonal Antibody (Cat# AAA435142)
Full Name
Lamin A/C Mouse mAb
Gene Names
LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; MADA; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Heart stained with Cytochrome C Monoclonal Antibody (CYCS/1010).)
Cytochrome C, Monoclonal Antibody (Cat# AAA439178)
Full Name
Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Rat.
Others not known
Others not known
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot
(Anti-ENPP2 Antibody (Center) at 1:2000 dilution + Human brain lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 99 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
ENPP2, Polyclonal Antibody (Cat# AAA9202571)
Full Name
ENPP2 Antibody (Center)
Gene Names
ENPP2; ATX; NPP2; ATX-X; PDNP2; LysoPLD; AUTOTAXIN; PD-IALPHA
Reactivity
Human
Applications
WB, EIA, FC/FACS
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(All lanes : Anti-OGN Antibody (N-term K75) at 1:500 dilutionLane 1: CCRF-CEM whole cell lysateLane 2: MCF-7 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 34 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
OGN, Polyclonal Antibody (Cat# AAA9202306)
Full Name
OGN Antibody (C-term)
Gene Names
OGN; OG; OIF; SLRR3A
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of lysates from A549,T47D cell line (from left to right), using VEGFR3 Antibody. It was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody.)
VEGFR3, Monoclonal Antibody (Cat# AAA9231865)
Full Name
VEGFR3 Antibody
Gene Names
FLT4; PCL; FLT-4; FLT41; LMPH1A; LMPHM1; VEGFR3; VEGFR-3
Reactivity
Human
Applications
WB, IHC, FC/FACS, EIA
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
GFRAL, Polyclonal Antibody (Cat# AAA9205922)
Full Name
GFRAL Antibody (C-term)
Gene Names
GFRAL; GRAL; UNQ9356; C6orf144; bA360D14.1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Peptide affinity purification
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
NTF97/Importin beta, Monoclonal Antibody (Cat# AAA435152)
Full Name
NTF97/Importin beta (835) Mouse mAb
Gene Names
KPNB1; IMB1; IPO1; IPOB; Impnb; NTF97
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Flow Cytometry (FC/FACS)
( Flow-cytometry using anti-CD22 antibody Epratuzumab Human lymphocytes were stained with an isotype control or the rabbit-chimeric version of Eptratuzumab at a concentration of 1 ug/ml for 30 mins at RT. After washing, bound antibody was detected using a AF488 conjugated donkey anti-rabbit antibody and cells analysed on a FACSCanto flow-cytometer.)
CD22, Monoclonal Recombinant Antibody (Cat# AAA488272)
Full Name
Anti-CD22 [hL22 (Epratuzumab)]
Gene Names
CD22; SIGLEC2; SIGLEC-2
Reactivity
Human, Rhesus Monkey, Cynomolgus Monkey
Applications
Blocking; IP; FC
Purity
Protein A affinity purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(All lanes : IDHP Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 51 KDa Observed band size :51 KDa Blocking/Dilution buffer : 1xTBST.)
IDHP, Monoclonal Antibody (Cat# AAA435187)
Full Name
IDHP Mouse mAb
Gene Names
IDH2; IDH; IDP; IDHM; IDPM; ICD-M; D2HGA2; mNADP-IDH
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Cytochrome C, Monoclonal Antibody (Cat# AAA438759)
Full Name
Cytochrome C (Mitochondrial Marker) Mouse Monoclonal Antibody
Gene Names
CYCS; CYC; HCS; THC4
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IF
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Anti-SCNN1A Antibody (Center) at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
SCNN1A, Polyclonal Antibody (Cat# AAA9214278)
Full Name
SCNN1A Antibody (Center)
Gene Names
SCNN1A; BESC2; ENaCa; SCNEA; SCNN1; ENaCalpha
Reactivity
Human
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(All lanes : ATPA Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 59 KDa Observed band size : 59 KDa Blocking/Dilution buffer : 1x TBST.)
ATP5A, Monoclonal Antibody (Cat# AAA435182)
Full Name
ATP5A (783) Mouse mAb
Gene Names
ATP5F1A; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PLA2G4A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)
cPLA2, Polyclonal Antibody (Cat# AAA125633)
Full Name
cPLA2 Polyclonal Antibody
Gene Names
PLA2G4A; PLA2G4; cPLA2-alpha
Reactivity
Human
Applications
WB, IHC, IF, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot
( Anti-VHL Antibody (N-term) at 1:2000 dilution + F9 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG,(H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 24 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
VHL, Polyclonal Antibody (Cat# AAA9206245)
Full Name
VHL Antibody (N-term)
Gene Names
VHL; RCA1; VHL1; pVHL; HRCA1
Reactivity
Human, mouse (Predicted Reactivity: Rat)
Applications
WB, EIA, IHC-P, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Test Data
(Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
FOXG1, Polyclonal Antibody (Cat# AAA9205338)
Full Name
FOXG1 Antibody (Center)
Gene Names
FOXG1; BF1; BF2; QIN; FKH2; HBF2; HFK1; HFK2; HFK3; KHL2; FHKL3; FKHL1; FKHL2; FKHL3; FKHL4; HBF-1; HBF-2; HBF-3; FOXG1A; FOXG1B; FOXG1C; HBF-G2
Reactivity
Human, mouse (Predicted Reactivity: Chicken, Rat, Xenopus, Drosophila)
Applications
WB, EIA, FC/FACS, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
CSF1R, Monoclonal Antibody (Cat# AAA378136)
Full Name
CSF1R (CD115)
Gene Names
CSF1R; FMS; CSFR; FIM2; HDLS; C-FMS; CD115; CSF-1R; M-CSF-R
Reactivity
Mouse
Applications
BL, FC/FACS
Purity
>95%
Pricing
$360/0.5 mg | $1,385/5x0.5 mg
Western Blot (WB)
(A dominant 59.6 kDa band and faint 87.6 kDa band for both the Hela wild type and knock out lysates were observed (3 ug/ml anti-PSEN1) vs the predicted size of 52.7 kDa. The molecular weight discrepancy could be due a post-translationally modified form of the target protein, a splice-variant form of the target protein, or an unrelated protein which shares the antibody-reactive epitope. The presence of bands in the knock out lysates suggest incomplete knockout of the target gene.)
Presenilin 1, Polyclonal Antibody (Cat# AAA9232251)
Full Name
Presenilin 1 Antibody (C-term) [Knockout Validated]
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Human, Mouse
Predicted: Rat, Monkey
Predicted: Rat, Monkey
Applications
WB, IHC, IF, FC/FACS
Pricing
$435/0.1 mL | $1,655/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-HMMR Antibody (C-term) at 1:2000 dilutionLane 1: K562 whole cell lysatesLane 2: LNCaP whole cell lysatesLane 3: HL-60 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 84 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
HMMR, Polyclonal Antibody (Cat# AAA9214366)
Full Name
HMMR Antibody (C-term)
Gene Names
HMMR; CD168; IHABP; RHAMM
Reactivity
Human
Applications
WB, IHC-P, FC, EIA
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using EDAR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
EDAR, Polyclonal Antibody (Cat# AAA129455)
Full Name
EDAR Polyclonal Antibody
Gene Names
EDAR; DL; ED3; ED5; ED1R; EDA3; HRM1; EDA1R; ECTD10A; ECTD10B; EDA-A1R
Reactivity
Human
Applications
WB, IHC, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling SFPQ with MBS435103 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, red). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as a secondary antibody.)
SFPQ, Monoclonal Antibody (Cat# AAA435103)
Full Name
SFPQ (578) Mouse mAb
Gene Names
SFPQ; PSF; POMP100; PPP1R140
Reactivity
Homo sapiens
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Immunofluorescence (IF)
(Immunofluorescence staining of fixed human peripheral blood monocytes (PBMs) with anti-Integrin beta-7 antibody FIB27 Immunofluorescence analysis of paraformaldehyde fixed human PBMs on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of FIB27 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
Integrin beta-7, Monoclonal Recombinant Antibody (Cat# AAA488443)
Full Name
Anti-Integrin beta-7 [FIB27]
Reactivity
Human, Mouse
Applications
FC/FACS, IP, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Nephrin (Y1210), Polyclonal Antibody (Cat# AAA9208203)
Full Name
Nephrin (Y1210) antibody
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
(Western blot analysis of extracts of mouse spleen, using MS4A7 antibody (MBS9135481) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
MS4A7, Polyclonal Antibody (Cat# AAA9135481)
Full Name
MS4A7 Polyclonal Antibody
Gene Names
MS4A7; CFFM4; MS4A8; 4SPAN2; CD20L4
Reactivity
Mouse
Applications
WB
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Anti-CD9 Antibody (Center)at 1:2000 dilution + MCF-7 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CD9, Polyclonal Antibody (Cat# AAA9202019)
Full Name
CD9 Antibody (Center)
Gene Names
CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29
Reactivity
Human
Applications
WB, EIA, IF, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Western Blot (WB)
(Anti-BCORL1 Antibody (N-term) at 1:500 dilution + HL-60 whole cell lysate.Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 183 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
BCORL1, Polyclonal Antibody (Cat# AAA9213215)
Full Name
BCORL1 Antibody (N-term)
Gene Names
BCORL1; BCoR-L1; CXorf10
Reactivity
Human
Applications
FC/FACS, EIA, WB, IHC-P
Purity
This antibody is purified through a protein A column, followed by peptide affinity purification.
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
CD3E, Monoclonal Antibody (Cat# AAA435170)
Full Name
CD3E (754) Mouse mAb
Gene Names
CD3E; T3E; TCRE; IMD18
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(All lanes : ACBD5 Mouse mAb at 1/2000 dilution Lane 1 : Jurkat cells membrane fraction Lane 2 : THP1 cells membrane fraction as negative control Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Mouse IgG-HRP, 5% milk conjugated at 1/10000 dilution Predicted band size : 60 KDa Observed band size : 60 KDa Blocking/Dilution buffer : 1x TBST.)
ACBD5, Monoclonal Antibody (Cat# AAA435111)
Full Name
ACBD5 Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Testing Data
(Published customer image: Oenothein B Primes bovine CD335+ cells to respond to IL-18. (A) Bovine PBMCs (105 cells/well) were depleted of CD335+ cells and treated with 20 ug/ml oenothein B or X-VIVO medium alone for 24 hrs. Cells were then washed and treated with 10 ng/ml rhu IL-18 or medium alone for 18 hrs. After incubation, IFN? levels in the supernatant fluids were measured by ELISA. The data are expressed as mean +/- SEM of three independent experiments comparing depleted PBMCs to un-depleted controls tested concurrently. All samples were tested in duplicate or triplicate. Statistical significance was measured by Two-way ANOVA with Bonferroni post-test. *p)
CD335, Monoclonal Antibody (Cat# AAA213799)
Full Name
MOUSE ANTI BOVINE CD335:RPE
Gene Names
NCR1; NKP46
Applications
FC/FACS
Pricing
$640/100 Tests | $2,735/5x100 Tests
Western Blot (WB)
(All lanes : Anti-CCK4 (PTK7) Antibody (N-term)at 1:1000-1:2000 dilutionLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: HepG2 whole cell lysateLane 4: Caco2 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 118 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
CCK4 (PTK7), Polyclonal Antibody (Cat# AAA9203896)
Full Name
CCK4 (PTK7) Antibody (N-term)
Gene Names
PTK7; CCK4; CCK-4
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(ATPA was immunoprecipitated from 1mg of THP1 cells membrane fraction, blotted with M25203 of 10 ug. Western blot was performed from the immunoprecipitate using M25203 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: THP1 cells membrane fraction. Lane2: IP product of THP1 cells membrane fraction. Blocking and dilution buffer and concentration:5% milk/TBST.)
ATP5A, Monoclonal Antibody (Cat# AAA435146)
Full Name
ATP5A Mouse mAb
Gene Names
ATP5PF; F6; CF6; ATP5; ATPM; ATP5A; ATP5J
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
IHC-P
(Staining PLA2G7 in human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)
PLA2G7, Polyclonal Antibody (Cat# AAA9205733)
Full Name
PLA2G7 Antibody (Center)
Gene Names
PLA2G7; PAFAD; PAFAH; LP-PLA2; LDL-PLA2
Reactivity
Human
Applications
IHC-P, FC, WB, ELISA
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
ATP5A, Monoclonal Antibody (Cat# AAA435097)
Full Name
ATP5A (529) Mouse mAb
Gene Names
ATP5F1A; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; ATP5A1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Anti-EZH2 Antibodyat 1:2000 dilution + Jurkat whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 85 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EZH2, Polyclonal Antibody (Cat# AAA9202800)
Full Name
EZH2 Antibody
Gene Names
EZH2; WVS; ENX1; EZH1; KMT6; WVS2; ENX-1; EZH2b; KMT6A
Reactivity
Human, mouse
Applications
WB, EIA, IHC, IF, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$425/0.4 mL | $190/0.08 mL | $1,665/5x0.4 mL
Western Blot (WB)
(Western blot analysis of MPZ Antibody (C-term) in CEM cell line lysates (35ug/lane). MPZ (arrow) was detected using the purified Pab.)
MPZ, Polyclonal Antibody (Cat# AAA9207336)
Full Name
MPZ Antibody (C-term)
Gene Names
MPZ; P0; CHM; DSS; MPP; CMT1; CMT1B; CMT2I; CMT2J; CMT4E; CMTDI3; CMTDID; HMSNIB
Reactivity
Human
Applications
EIA, IHC-p, WB, FC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
Western Blot (WB)
P5CS, Monoclonal Antibody (Cat# AAA435154)
Full Name
P5CS (012) Mouse mAb
Gene Names
ALDH18A1; GSAS; P5CS; PYCS; SPG9; ADCL3; SPG9A; SPG9B; ARCL3A
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ITGB4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
ITGB4, Polyclonal Antibody (Cat# AAA126040)
Full Name
ITGB4 Polyclonal Antibody
Gene Names
ITGB4; CD104
Reactivity
Human, Mouse
Applications
WB, IHC, FC/FACS
Purity
Affinity Purification
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Western Blot (WB)
(Western blot analysis of extracts of SH-SY5Y cells, using NR4A1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
Nur77, Polyclonal Antibody (Cat# AAA125048)
Full Name
Nur77
Gene Names
NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77
Reactivity
Human, mouse, rat
Applications
WB, FC/FACS
Pricing
$180/0.02 mL | $210/0.05 mL | $285/0.1 mL | $430/0.2 mL | $1,790/5x0.2 mL
Immunofluorescence (IF)
( Immunofluorescence staining of fixed HepG2 cells with anti-DARC antibody 2C3 Immunofluorescence analysis of paraformaldehyde fixed HepG2 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 2C3 at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing cytoplasmic staining, likely of endosomes, and some membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.)
DARC, Monoclonal Recombinant Antibody (Cat# AAA488374)
Full Name
Anti-DARC [2C3]
Reactivity
Human
Applications
EIA, FC/FACS, BL
Purity
Protein A Affinity Purified
Pricing
$495/0.2 mg | $2,025/5x0.2 mg
Western Blot (WB)
ATP synthase subunit gamma, Monoclonal Antibody (Cat# AAA435251)
Full Name
ATP synthase subunit gamma Mouse mAb
Reactivity
Human
Applications
WB, IP, FC/FACS
Pricing
$260/0.02 mL | $405/0.05 mL | $650/0.1 mL | $2,645/5x0.1 mL
Western Blot (WB)
(Western blot analysis of TOX3 Antibody (Center) in mouse stomach tissue lysates (35ug/lane). TOX3 (arrow) was detected using the purified Pab.)
TOX3, Polyclonal Antibody (Cat# AAA9203659)
Full Name
TOX3 Antibody (Center)
Gene Names
TOX3; CAGF9; TNRC9
Reactivity
Human, mouse
Applications
WB, EIA, FC/FACS, IHC
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Flow Cytometry (FC/FACS)
(Overlay histogram showing ZF4 cells stained at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/400 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
ak2, Polyclonal Antibody (Cat# AAA9232174)
Full Name
Zebrafish ak2 Antibody (N-term)
Gene Names
ak2; wu:fb34f05; wu:fj80e03
Reactivity
Zebrafish
Applications
WB, EIA
Pricing
$425/0.1 mL | $1,615/5x0.1 mL
Western Blot (WB)
(TMEM100 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TMEM100 antibody detected the TMEM100 protein (arrow).)
TMEM100, Polyclonal Antibody (Cat# AAA9212673)
Full Name
TMEM100 Antibody (C-term)
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
WB, EIA, IHC-P, FC/FACS
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
