Western Blot (WB)
Mouse anti-Human ATP5A Monoclonal Antibody | anti-ATP5A antibody
ATP5A (529) Mouse mAb
IP: 10 tests
FC/FACS: 1:100-1:200
Western Blot (WB)
Western Blot (WB)
Immunoprecipitation (IP)
(ATPA(529) was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25065 of 10 ug. Western blot was performed from the immunopre-cipitate using M25065 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 60 KDaObserved band size : 60 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(ATPA(529) was immunoprecipitated from 1mg of Jurkat cells membrane fraction, blotted with M25065 of 10 ug. Western blot was performed from the immunopre-cipitate using M25065 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: Jurkat cells membrane fraction. Lane2: IP product of Jurkat cells membrane fraction.Predicted band size : 60 KDaObserved band size : 60 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
Flow Cytometry (FC/FACS)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]
Uniprot Description
Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites ().
Research Articles on ATP5A
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Product Notes
The ATP5A atp5f1a (Catalog #AAA435097) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The ATP5A (529) Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ATP5A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200. Researchers should empirically determine the suitability of the ATP5A atp5f1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATP5A, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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