Test Data
(Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit FOXG1 Polyclonal Antibody | anti-FOXG1 antibody
FOXG1 Antibody (Center)
FC~~1:10~50
IF~~1:10~50
Test Data
(Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Test Data
(Anti-FOXG1 Antibody (Center) at 1:1000 dilution + human brain lysate Lysates/proteins at 20 ?g per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 52 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Western blot analysis of FOXG1 Antibody (Center) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of FOXG1 Antibody (Center) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab.)
Flow Cytometry (FC/FACS)
(FOXG1 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(FOXG1 Antibody (Center) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunofluorescence (IF)
(Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.)
Immunofluorescence (IF)
(Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly.)
Philippe, C., et al. J. Med. Genet. 47(1):59-65(2010)
Ariani, F., et al. Am. J. Hum. Genet. 83(1):89-93(2008)
Adesina, A.M., et al. J. Neurooncol. 85(2):111-122(2007)
Bredenkamp, N., et al. Dev. Genes Evol. 217(3):227-233(2007)
NCBI and Uniprot Product Information
NCBI Description
This locus encodes a member of the forked-head transcription factor family. The encoded protein, which functions as a repressor, may play a role in brain development. Mutations at this locus have been associated with Rett syndrome. [provided by RefSeq, Feb 2012]
Uniprot Description
FOXG1: Transcription repression factor which plays an important role in the establishment of the regional subdivision of the developing brain and in the development of the telencephalon. Interacts with KDM5B. Expression is restricted to the neurons of the developing telencephalon.
Protein type: Nuclear receptor co-regulator; DNA-binding; Transcription factor
Chromosomal Location of Human Ortholog: 14q13
Cellular Component: nucleus
Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor activity
Biological Process: transcription, DNA-dependent; brain development; negative regulation of transcription, DNA-dependent; aging
Disease: Rett Syndrome, Congenital Variant
Research Articles on FOXG1
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Product Notes
The FOXG1 foxg1 (Catalog #AAA9205338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 225-252. The FOXG1 Antibody (Center) reacts with Human, mouse (Predicted Reactivity: Chicken, Rat, Xenopus, Drosophila) and may cross-react with other species as described in the data sheet. AAA Biotech's FOXG1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF). WB~~1:1000 FC~~1:10~50 IF~~1:10~50. Researchers should empirically determine the suitability of the FOXG1 foxg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "FOXG1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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