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Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Rabbit anti-Human, mouse Nephrin (Y1210) Polyclonal Antibody | anti-NPHS1 antibody
Nephrin (Y1210) antibody
Gene Names
NPHS1; CNF; NPHN; nephrin
Reactivity
Human, mouse
Applications
Western Blot, ELISA, Flow Cytometry, Functional Assay
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Nephrin (Y1210); Polyclonal Antibody; Nephrin (Y1210) antibody; Nephrin; Renal glomerulus-specific cell adhesion receptor; NPHS1; NPHN; anti-NPHS1 antibody
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This Nephrin antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1191-1219 amino acids from human Nephrin.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
1191-1219
Applicable Applications for anti-NPHS1 antibody
Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:2000
FC~~1:10~50
FC~~1:10~50
Function
Seems to play a role in the development orfunction of the kidney glomerular filtration barrier. Regulates glomerular vascular permeability. May anchor the podocyte slitdiaphragm to the actin cytoskeleton. Plays a role in skeletal muscle formation through regulation of myoblast fusion (By similarity).
Cellular Location
Cell membrane; Single-pass type I membrane protein. Note=Predominantly located atpodocyte slit diaphragm between podocyte foot processes. Also associated with podocyteapical plasma membrane.
Tissue Location
Specifically expressed in podocytes of kidney glomeruli
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203) western blot analysis in MDA-MB435 cell line lysates (35ug/lane). This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203)western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Western Blot (WB)
(Nephrin (Y1210) antibody (Cat. #MBS9208203)western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Nephrin antibody detected the Nephrin protein (arrow).)
Western Blot (WB)
(All lanes : Anti-Nephrin (Y1210) antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A-498 whole cell lysateLane 3: Caki-1whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(All lanes : Anti-Nephrin (Y1210) antibody at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A-498 whole cell lysateLane 3: Caki-1whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution.Predicted band size : 135 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC)
(Nephrin Antibody (Y1210) (Cat. #MBS9208203)flow cytometric analysis of MDA-MB435 cells(right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugatedgoat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC)
(Nephrin Antibody (Y1210) (Cat. #MBS9208203)flow cytometric analysis of MDA-MB435 cells(right histogram) compared to a negativecontrol cell (left histogram). FITC-conjugatedgoat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-NPHS1 antibody
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes.
Product Categories/Family for anti-NPHS1 antibody
References
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010)
Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010)
Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)
Aya, K., et al. Kidney Int. 57(2):401-404(2000)
Wu, F., et al. J. Am. Soc. Nephrol. 21(9):1456-1467(2010)
Tossidou, I., et al. J. Biol. Chem. 285(33):25285-25295(2010)
Machuca, E., et al. J. Am. Soc. Nephrol. 21(7):1209-1217(2010)
Aya, K., et al. Kidney Int. 57(2):401-404(2000)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
nephrin
NCBI Official Synonym Full Names
nephrosis 1, congenital, Finnish type (nephrin)
NCBI Official Symbol
NPHS1
NCBI Official Synonym Symbols
CNF; NPHN; nephrin
NCBI Protein Information
nephrin
UniProt Protein Name
Nephrin
Protein Family
UniProt Gene Name
NPHS1
UniProt Synonym Gene Names
NPHN
UniProt Entry Name
NPHN_HUMAN
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Product Notes
The NPHS1 nphs1 (Catalog #AAA9208203) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1191-1219. The Nephrin (Y1210) antibody reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Nephrin (Y1210) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS). WB~~1:2000 FC~~1:10~50. Researchers should empirically determine the suitability of the NPHS1 nphs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Nephrin (Y1210), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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