Western Blot
( All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit Prohibitin (PHB1) Polyclonal Antibody | anti-PHB antibody
Prohibitin (PHB1) Antibody (N-term)
Predicted Reactivity: Bovine, Chicken
Predicted Reactivity: Bovine, Chicken
IHC-P~~1:100
FC~~1:10~50
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot
( All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot
( All lanes : Anti-Prohibitin (PHB1) Antibody (N-term) at 1:2000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole celllysate Lane 3: A431 whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: Hela whole cell lysate Lane 6: Mouse kidney tissue lysate Lane 7: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 30 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of ATDC5 cells using PHB1 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Immunohistochemistry
(Immunohistochemical analysis of MBS9202298 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemistry
(Immunohistochemical analysis of MBS9202298 on paraffin-embedded Human liver tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody (1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemistry
(Immunohistochemical analysis of MBS9202298 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
Immunohistochemistry
(Immunohistochemical analysis of MBS9202298 on paraffin-embedded Human kidney tissue. Tissue was fixed with formaldehyde at room temperature. Heat induced epitope retrieval was performed by EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:100) for 1 hour at room temperature. Undiluted CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene is evolutionarily conserved, and its product is proposed to play a role in human cellular senescence and tumor suppression. Antiproliferative activity is reported to be localized to the 3' UTR, which is proposed to function as a trans-acting regulatory RNA. Several pseudogenes of this gene have been identified. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]
Uniprot Description
PHB: a pleiotropic membrane protein that suppresses cell proliferation, apoptosis and senescence. It plays a role both in maintaining mitochondrial integrity and in cell cycle regulation. Expression increases approximately 3-fold upon entry into G1 phase compared with other phases of the cell cycle. It helps prevent ROS-induced senescence and its expression decreases heterogeneously during cellular aging. Helps maintain the angiogenic capacity of endothelial cells. Up-regulated during activation of primary human T cells via CD3/CD28 pathways. Present in multiple cellular compartments. Large assemblies of PHB and PHB2 localize in the inner membrane of mitochondria. Acts as a mitochondrial chaperone protein, regulates mitochondrial morphogenesis, and helps regulate the organization of mitochondrial DNA. Reported to target lipid rafts. In the nucleus, it has been reported to interact with various transcription factors, modulating their activity. A potential tumor suppressor that functions as a potent transcriptional corepressor for estrogen receptor alpha. It is downregulated by androgens, and represses androgen receptor activity. Co-localizes with Rb in the nucleus and recruits N-CoR and HDAC1 for transcriptional repression. In vivo promoter occupancy studies have suggested that the PHB gene is a direct target of c-Myc. May bind to and enhance the transcriptional activity of p53. Both Phb and Phb2 are present in the circulation and can be internalized by cultured cells. Overexpressed in endometrioid ovarian adenocarcinoma and papillary serous ovarian carcinoma. Mutated in sporadic breast cancer.
Protein type: Apoptosis; Transcription, coactivator/corepressor; Cell cycle regulation; Nuclear receptor co-regulator; Oncoprotein; Chaperone; Mitochondrial; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 17q21
Cellular Component: nucleoplasm; membrane; mitochondrion; integral to plasma membrane; cytoplasm; mitochondrial inner membrane; nucleus
Molecular Function: protein binding; enzyme binding; histone deacetylase binding
Biological Process: osteoblast differentiation; regulation of apoptosis; transcription from RNA polymerase II promoter; negative regulation of cell proliferation; progesterone receptor signaling pathway; regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; histone deacetylation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of cell growth; negative regulation of transcription, DNA-dependent; signal transduction
Disease: Breast Cancer
Research Articles on PHB
Similar Products
Product Notes
The PHB phb (Catalog #AAA9202298) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 92-121. The Prohibitin (PHB1) Antibody (N-term) reacts with Human, Mouse Predicted Reactivity: Bovine, Chicken and may cross-react with other species as described in the data sheet. AAA Biotech's Prohibitin (PHB1) can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistochemistry Paraffin (IHC-P). WB~~1:2000 IHC-P~~1:100 FC~~1:10~50. Researchers should empirically determine the suitability of the PHB phb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Prohibitin (PHB1), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
