Immunoprecipitation (IP)
(PRKDC was immunoprecipitated from 1mg of HepG2 cells membrane fraction, blotted with MBS435247 of 10 ug. Western blot was performed from the immunopre-cipitate using MBS435247 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: HepG2 cells membrane fraction. Lane2: IP product of HepG2 cells membrane fraction.Predicted band size : 469 KDaObserved band size : 469 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Mouse anti-Human DNA PKcs Monoclonal Antibody | anti-DNAPK antibody
DNA PKcs Mouse mAb
Reactivity
Human
Applications
Western Blot, Immunoprecipitation, Flow Cytometry, Functional Assay
Synonyms
DNA PKcs; Monoclonal Antibody; DNA PKcs Mouse mAb; anti-DNAPK antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG
Applicable Applications for anti-DNAPK antibody
Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS)
Application Notes
WB: 1:1000-1:5000
IP: 10 tests
FC/FACS: 1:100-1:200
*For Western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween- 20 at 4°C with gentle shaking overnight.
IP: 10 tests
FC/FACS: 1:100-1:200
*For Western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween- 20 at 4°C with gentle shaking overnight.
Source Note
This monoclonal antibody is produced by immunizing mice with a sythetic polypeptide corresponding to PRKDC protein.
Preparation and Storage
Store at -20 degree C. Stable for one year from the date of shipment.
Immunoprecipitation (IP)
(PRKDC was immunoprecipitated from 1mg of HepG2 cells membrane fraction, blotted with MBS435247 of 10 ug. Western blot was performed from the immunopre-cipitate using MBS435247 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: HepG2 cells membrane fraction. Lane2: IP product of HepG2 cells membrane fraction.Predicted band size : 469 KDaObserved band size : 469 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Immunoprecipitation (IP)
(PRKDC was immunoprecipitated from 1mg of HepG2 cells membrane fraction, blotted with MBS435247 of 10 ug. Western blot was performed from the immunopre-cipitate using MBS435247 at 1/1000 dilution. Anti-Mouse-IgG(HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/10000 dilution. Lane 1: HepG2 cells membrane fraction. Lane2: IP product of HepG2 cells membrane fraction.Predicted band size : 469 KDaObserved band size : 469 KDaBlocking and dilution buffer and concentration:5% milk/TBST.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (liver hepatocelluar carcinoma) cells labeling PRKDC with MBS435247 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Flow Cytometry (FC/FACS)
(Flow cytometric analysis of 2% paraformaldehyde-fixed HepG2 (liver hepatocelluar carcinoma) cells labeling PRKDC with MBS435247 at 1/200 dilution (green) compared with a mouse monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody, blue). Goat anti-mouse IgG (FITC) at 1/300 dilution was used as the secondary antibody.)
Related Product Information for anti-DNAPK antibody
This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.
Product Categories/Family for anti-DNAPK antibody
Similar Products
Product Notes
The DNAPK (Catalog #AAA435247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The DNA PKcs Mouse mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's DNA PKcs can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS). WB: 1:1000-1:5000 IP: 10 tests FC/FACS: 1:100-1:200 *For Western blots, incubate membrane with diluted antibody in 5% w/v nonfat dry milk, 1x TBS, 0.05% Tween- 20 at 4°C with gentle shaking overnight. Researchers should empirically determine the suitability of the DNAPK for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA PKcs, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
