Rabbit TMEM100 Polyclonal Antibody | anti-TMEM100 antibody

TMEM100 Antibody (C-term)

Cat. Num. AAA9212673

• Reactivity: Human (Predicted Reactivity: Bovine)
• Applications: Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TMEM100; Polyclonal Antibody; TMEM100 Antibody (C-term); Transmembrane protein 100; anti-TMEM100 antibody

• Host: Rabbit
• Reactivity: Human (Predicted Reactivity: Bovine)
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TMEM100 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 93-122 amino acids from the C-terminal region of human TMEM100.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 93-122
• Sequence Length: 134

• Applicable Applications for anti-TMEM100 antibody: Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:2000; IHC-P~~1:50~100; FC~~1:10~50
• Function: Plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. Involved in the regulation of nociception, acting as a modulator of the interaction between TRPA1 and TRPV1, two molecular sensors and mediators of pain signals in dorsal root ganglia (DRG) neurons. Mechanistically, it weakens their interaction, thereby releasing the inhibition of TRPA1 by TRPV1 and increasing the single-channel open probability of the TRPA1-TRPV1 complex.
• Cellular Location: Cell membrane; Multi-pass membrane protein. Membrane; Multi-pass membrane protein Perikaryon. Cytoplasm, perinuclear region. Endoplasmic reticulum Note=Colocalized with HSPA5 in the endoplasmic reticulum (ER) Enriched in ER microsome. Colocalized with BMP4 in neural cell bodies and neural fibers of the enteric nervous system.
• Tissue Location: Expressed in neurons of the myenteric and submucosal plexuses in the gastric body, jejunum and proximal colon. Expressed in arterial endothelial cells and neurons of the central nervous system and peripheral nervous system. Expressed in umbilical artery endothelial cells (at protein level)
• Preparation and Storage: Maintain refrigerated at 2-8°C. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(TMEM100 Antibody (C-term) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the TMEM100 antibody detected the TMEM100 protein (arrow).)

Immunohistochemistry (IHC)

(TMEM100 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TMEM100 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(TMEM100 Antibody (C-term) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Western Blot (WB)

(All lanes : Anti-TMEM100 Antibody (C-term) at 1:2000 dilution Lane 1: A549 whole cell lysate Lane 2: NCI-H460 whole cell lysate Lane 3: SH-SY5Y whole cell lysate Lane 4: U-87 MG whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-RabbitIgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 14 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-TMEM100 antibody

Cell Biology

References

de Krom, M., et al. Biol. Psychiatry 65(7):625-630(2009)
Wang, A.G., et al. Biochem. Biophys. Res. Commun. 345(3):1022-1032(2006)

NCBI and Uniprot Product Information

• NCBI GI #: 153792480
• NCBI GeneID: 55273
• NCBI Accession #: NP_001093110.1; NP_060756.2
• NCBI GenBank Nucleotide #: NM_001099640.1
• UniProt Accession #: Q9NV29; Q96FZ0; D3DTY7; I3L214
• Molecular Weight: 14386
• NCBI Official Full Name: transmembrane protein 100
• NCBI Official Synonym Full Names: transmembrane protein 100
• NCBI Official Symbol: TMEM100
• NCBI Protein Information: transmembrane protein 100
• UniProt Protein Name: Transmembrane protein 100
• Protein Family: Transmembrane protein
• UniProt Gene Name: TMEM100
• UniProt Entry Name: TM100_HUMAN

Uniprot Description

TMEM100: Plays a role during embryonic arterial endothelium differentiation and vascular morphogenesis through the ACVRL1 receptor-dependent signaling pathway upon stimulation by bone morphogenetic proteins, such as GDF2/BMP9 and BMP10. Up-regulated by GDF2/BMP9 and BMP10 (at protein level)

Protein type: Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 17q22

Cellular Component: perinuclear region of cytoplasm; endoplasmic reticulum; integral to membrane; plasma membrane; perikaryon

Biological Process: BMP signaling pathway; Notch signaling pathway; positive regulation of endothelial cell differentiation; in utero embryonic development; protein kinase B signaling cascade; angiogenesis; vasculogenesis