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Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human GFRAL Polyclonal Antibody | anti-GFRAL antibody
GFRAL Antibody (C-term)
Gene Names
GFRAL; GRAL; UNQ9356; C6orf144; bA360D14.1
Reactivity
Human
Applications
Western Blot, ELISA, Immunohistochemistry, Flow Cytometry, Functional Assay
Purity
Peptide affinity purification
Synonyms
GFRAL; Polyclonal Antibody; GFRAL Antibody (C-term); GDNF family receptor alpha-like; C6orf144; anti-GFRAL antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This GFRAL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 366-394 amino acids from the C-terminal region of human GFRAL.
Purity/Purification
Peptide affinity purification
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Sequence Positions
366-394
Sequence Length
394
Applicable Applications for anti-GFRAL antibody
Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
Application Notes
WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50
IHC-P~~1:50~100
FC~~1:10~50
Function
Brainstem-restricted receptor for GDF15 which regulates food intake, energy expenditure and body weight in response to metabolic and toxin-induced stresses (PubMed:28953886, PubMed:28846097, PubMed:28846098, PubMed:28846099). Upon interaction with its ligand, GDF15, interacts with RET and induces cellular signaling through activation of MAPK- and AKTsignaling pathways.
Cellular Location
Cell membrane; Single-pass membrane protein; Extracellular side
Tissue Location
Expressed in the brainstem, restricted to cells in the area postrema and the immediately adjacent region of the nucleus tractus solitarius (at protein level) (PubMed:28846097, PubMed:28846098). Detected at low levels in testis and adipose tissue (PubMed:28846097).
Preparation and Storage
Maintain refrigerated at 2-8°C for up to 2weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + MDA-MB-453 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 45 kDa Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + Human brain whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),)
Western Blot (WB)
(Anti-GFRAL Antibody (C-term) at 1:1000 dilution + Human brain whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),)
Western Blot (WB)
(GFRAL Antibody (C-term) (MBS9205922) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GFRAL antibody detected the GFRAL protein (arrow).)
Western Blot (WB)
(GFRAL Antibody (C-term) (MBS9205922) western blot analysis in MDA-MB435 cell line lysates (35ug/lane).This demonstrates the GFRAL antibody detected the GFRAL protein (arrow).)
Immunohistohchemistry (IHC)
(GFRAL antibody (C-term) (MBS9205922) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFRAL antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistohchemistry (IHC)
(GFRAL antibody (C-term) (MBS9205922) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the GFRAL antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC)
(GFRAL Antibody (C-term) (MBS9205922) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (Rabbit IgG isotype) (left histogram).FITC-conjugated goat-anti-rabbit secondary.)
Flow Cytometry (FC)
(GFRAL Antibody (C-term) (MBS9205922) flow cytometric analysis of MDA-MB435 cells (right histogram) compared to a negative control (Rabbit IgG isotype) (left histogram).FITC-conjugated goat-anti-rabbit secondary.)
Product Categories/Family for anti-GFRAL antibody
References
Fellay, J., et al. PLoS Genet. 5 (12), E1000791 (2009) :
Li, Z., et al. J. Neurochem. 95(2):361-376(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
Li, Z., et al. J. Neurochem. 95(2):361-376(2005)
Mungall, A.J., et al. Nature 425(6960):805-811(2003)
Clark, H.F., et al. Genome Res. 13(10):2265-2270(2003)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
GDNF family receptor alpha-like
NCBI Official Synonym Full Names
GDNF family receptor alpha like
NCBI Official Symbol
GFRAL
NCBI Official Synonym Symbols
GRAL; UNQ9356; C6orf144; bA360D14.1
NCBI Protein Information
GDNF family receptor alpha-like
UniProt Protein Name
GDNF family receptor alpha-like
Protein Family
UniProt Gene Name
GFRAL
UniProt Synonym Gene Names
C6orf144
UniProt Entry Name
GFRAL_HUMAN
Uniprot Description
GFRAL: Belongs to the GDNFR family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 6p12.1
Cellular Component: integral to membrane; plasma membrane
Biological Process: stress-activated protein kinase signaling pathway; negative regulation of neuron apoptosis
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Product Notes
The GFRAL gfral (Catalog #AAA9205922) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 366-394. The GFRAL Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's GFRAL can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000 IHC-P~~1:50~100 FC~~1:10~50. Researchers should empirically determine the suitability of the GFRAL gfral for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "GFRAL, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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