Rabbit anti-Human, mouse TOX3 Polyclonal Antibody | anti-TOX3 antibody

TOX3 Antibody (Center)

Cat. Num. AAA9203659

• Gene Names: TOX3; CAGF9; TNRC9
• Reactivity: Human, mouse
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay, Immunohistochemistry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: TOX3; Polyclonal Antibody; TOX3 Antibody (Center); TOX high mobility group box family member 3; CAG trinucleotide repeat-containing gene F9 protein; Trinucleotide repeat-containing gene 9 protein; CAGF9; TNRC9; anti-TOX3 antibody

• Host: Rabbit
• Reactivity: Human, mouse
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This TOX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 214-242 amino acids from the Central region of human TOX3.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.48 (varies by lot)
• Sequence Positions: 214-242
• Sequence Length: 571

• Applicable Applications for anti-TOX3 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of TOX3 Antibody (Center) in mouse stomach tissue lysates (35ug/lane). TOX3 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(TOX3 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the TOX3 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Western Blot (WB)

(Western blot analysis of TOX3 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in MDA-MB453 cell line lysate. TOX3 Antibody (Center) (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of TOX3 Antibody (Center) Pab pre-incubated without(lane 1) and with(lane 2) blocking peptide in mouse lung tissue lysate. TOX3 Antibody (Center) (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(TOX3 Antibody (Center) flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-TOX3 antibody

TOX3 contains an HMG-box, indicating that it may be involved in bending and unwinding of DNA and alteration of chromatin structure. The C-terminus of the encoded protein is glutamine-rich due to CAG repeats in the coding sequence. A minor allele of this gene has been implicated in an elevated risk of breast cancer.

References

Wacholder, S., et al. N. Engl. J. Med. 362(11):986-993(2010)
Odefrey, F., et al. Cancer Res. 70(4):1449-1458(2010)
Couch, F.J., et al. Cancer Epidemiol. Biomarkers Prev. 18(11):3044-3048(2009)
Stacey, S.N., et al. Nat. Genet. 39(7):865-869(2007)

NCBI and Uniprot Product Information

• NCBI GI #: 226054295
• NCBI GeneID: 27324
• NCBI Accession #: NP_001073899.2; NP_001139660.1
• NCBI GenBank Nucleotide #: NM_001080430.2
• UniProt Accession #: O15405; B4DRD0; B5MCW4
• Molecular Weight: 63342
• NCBI Official Full Name: TOX high mobility group box family member 3 isoform 1
• NCBI Official Synonym Full Names: TOX high mobility group box family member 3
• NCBI Official Symbol: TOX3
• NCBI Official Synonym Symbols: CAGF9; TNRC9
• NCBI Protein Information: TOX high mobility group box family member 3
• UniProt Protein Name: TOX high mobility group box family member 3
• Protein Family: TOX high mobility group box family
• UniProt Gene Name: TOX3
• UniProt Synonym Gene Names: CAGF9; TNRC9
• UniProt Entry Name: TOX3_HUMAN

NCBI Description

The protein encoded by this gene contains an HMG-box, indicating that it may be involved in bending and unwinding of DNA and alteration of chromatin structure. The C-terminus of the encoded protein is glutamine-rich due to CAG repeats in the coding sequence. A minor allele of this gene has been implicated in an elevated risk of breast cancer. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Apr 2009]

Uniprot Description

TOX3: Transcriptional coactivator of the p300/CBP-mediated trancription complex. Activates transactivation through cAMP response element (CRE) sites. Protects against cell death by inducing antiapoptotic and repressing pro-apoptotic transcripts. Stimulates transcription from the estrogen-responsive or BCL-2 promoters. Required for depolarization-induced transcription activation of the C-FOS promoter in neurons. Associates with chromatin to the estrogen-responsive C3 promoter region. 2 isoforms of the human protein are produced by alternative splicing.

Chromosomal Location of Human Ortholog: 16q12.1

Cellular Component: nucleus

Molecular Function: protein binding; protein homodimerization activity; estrogen response element binding; phosphoprotein binding; chromatin binding

Biological Process: chromatin remodeling; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; negative regulation of neuron apoptosis