Filters
Clonality
Type
Reactivity
Gene Name
Isotype
Host
Application
Clone
10000 results for "actin" - showing 1-50
Typical Testing Data/Standard Curve (for reference only)
Gelsolin (GS), ELISA Kit (Cat# AAA264815)
Full Name
Human Gelsolin (GS) ELISA Kit
Reactivity
Human
Pricing
$275/48-Strip-Wells | $395/96-Strip-Wells | $1,660/5x96-Strip-Wells | $3,030/10x96-Strip-Wells
Actin, Native Protein (Cat# AAA143069)
Full Name
Actin
Purity
Greater than 98.0% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $1,925/1 mg | $8,435/5x1 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human skeletal muscle stained with MBS602996 using peroxidase-conjugate and DAB chromogen. Note cytoplasmic staining of skeletal muscle cells.)
a-Actin, Skeletal Muscle, Monoclonal Antibody (Cat# AAA602996)
Full Name
a-Actin, Skeletal Muscle
Reactivity
Bovine, Frog, Guinea Pig, Human, Rabbit, Rat, Sheep
Applications
IHC, IF
Purity
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Pricing
$620/0.5 mL | $2,785/5x0.5 mL
Plectin, Polyclonal Antibody (Cat# AAA834370)
Full Name
Plectin antibody
Gene Names
PLEC; HD1; PCN; EBS1; EBSO; PLTN; PLEC1; LGMD2Q; PLEC1b
Applications
IHC, WB
Actin-like protein 7B (ACTL7B), ELISA Kit (Cat# AAA7237978)
Full Name
Guinea pig Actin-like protein 7B (ACTL7B) ELISA Kit
Gene Names
ACTL7B; Tact1
Reactivity
Guinea Pig
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Actin-like protein 7B (ACTL7B), ELISA Kit (Cat# AAA7234989)
Full Name
Porcine Actin-like protein 7B (ACTL7B) ELISA Kit
Gene Names
ACTL7B; Tact1
Reactivity
Porcine
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
()
Actin Alpha 2, Smooth Muscle (ACTa2), ELISA Kit (Cat# AAA2025158)
Full Name
Human Actin Alpha 2, Smooth Muscle (ACTa2) ELISA Kit
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Pricing
$525/48-Strip-Wells | $695/96-Strip-Wells | $2,810/5x96-Strip-Wells | $5,125/10x96-Strip-Wells | $345/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Gelsolin (GS), ELISA Kit (Cat# AAA2023011)
Full Name
Human Gelsolin (GS) ELISA Kit
Reactivity
Human
Pricing
$445/48-Strip-Wells | $590/96-Strip-Wells | $2,395/5x96-Strip-Wells | $4,375/10x96-Strip-Wells | $305/24-Strip-Wells
Typical Testing Data/Standard Curve (for reference only)
Alpha Smooth Muscle Actin, ELISA Kit (Cat# AAA731995)
Full Name
Human Alpha Smooth Muscle Actin ELISA Kit
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Human
Pricing
$475/48-Strip-Wells-(Competitive) | $680/96-Strip-Wells-(Competitive) | $475/48-Strip-Wells-(Sandwich) | $680/96-Strip-Wells-(Sandwich) | $3,010/5x96-Strip-Wells-(Competitive) | $5,675/10x96-Strip-Wells-(Competitive) | $3,010/5x96-Strip-Wells-(Sandwich) | $5,675/10x96-Strip-Wells-(Sandwich)
Quality Control
(Western blot analysis of immunized recombinant protein, using anti-ACTB monoclonal antibody. )
ACTB, Monoclonal Antibody (Cat# AAA120059)
Full Name
Mouse monoclonal antibody Anti-Human ACTB
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human
Applications
DB, ICC, WB, FC/FACS
Pricing
$460/0.1 mg | $2,070/5x0.1 mg
F-actin-capping protein subunit alpha-1 (CAPZA1), Recombinant Protein (Cat# AAA962390)
Full Name
Recombinant Chicken F-actin-capping protein subunit alpha-1 (CAPZA1)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,715/1 mg (E-Coli) | $2,190/1 mg (Yeast) | $3,270/1 mg (Baculovirus) | $1,660/0.1 mg (Baculovirus) | $2,245/0.1 mg (Mammalian-Cell) | $955/0.1 mg (E-Coli) | $1,110/0.1 mg (Yeast) | $5,935/0.5 mg (Mammalian-Cell) | $1,155/0.02 mg (Baculovirus) | $1,420/0.02 mg (Mammalian-Cell) | $795/0.02 mg (E-Coli) | $945/0.02 mg (Yeast)
Actin (N-terminal Region) Peptide, Peptide (Cat# AAA474001)
Full Name
Actin (N-terminal Region) Peptide
Pricing
$160/0.05 mg | $565/5x0.05 mg
Western Blot (WB)
(Western blot detection of beta actin in C6, 3T3, COS7, CHO-k1, K562 and Goat muscle cell lysates using beta actin mouse mAb (1:10000 diluted).Predicted band size: 45KDa.Observed band size: 45KDa.)
beta-Actin, Monoclonal Antibody (Cat# AAA475037)
Full Name
Anti-beta-Actin Mouse mAb
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Monkey, Goat, Rat, Hamster, Saccharomyces cerevisiae, Arabidopsis, Pichia pastoris, Rabbit, Zebrafish, Drosophila, Leaf of Rice, Fruit of Cucumber, Chlamydomonas Reinhardtii, Fish, Pig, Chicken
Applications
WB
Purity
Affinity purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Bmf (NT), Polyclonal Antibody (Cat# AAA194181)
Full Name
Bmf (NT) Polyclonal Antibody
Applications
Immunoblot
Pricing
$475/0.1 mg | $2,090/5x0.1 mg
Alpha Actinin, Native Protein (Cat# AAA143070)
Full Name
Alpha Actinin
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $2,635/1 mg | $11,635/5x1 mg
Testing Data
(Background)
Chick Embryo Extract, Ultrafiltrate, Reagent (Cat# AAA635451)
Full Name
Chick Embryo Extract, Ultrafiltrate (CEE)
Purity
Molecular Biology Grade
Pricing
$420/20 mL | $935/100 mL | $4,210/5x100 mL
Filamin, Native Protein (Cat# AAA143073)
Full Name
Filamin
Gene Names
FLNA; FLN; FMD; MNS; OPD; ABPX; CSBS; CVD1; FLN1; NHBP; OPD1; OPD2; XLVD; XMVD; FLN-A; ABP-280
Purity
Greater than 90.0% as determined by SDS-PAGE.
Pricing
$180/0.01 mg | $250/0.05 mg | $2,230/1 mg | $9,805/5x1 mg
Western Blot (WB)
(Sample: Recombinant FLNC, Human)
Filamin C Gamma, Polyclonal Antibody (Cat# AAA2026155)
Full Name
Polyclonal Antibody to Filamin C Gamma (FLNC)
Gene Names
FLNC; ABPA; ABPL; FLN2; MFM5; MPD4; RCM5; CMH26; ABP-280; ABP280A
Reactivity
Homo sapiens (Human)
Applications
WB, IHC, ICC, IP
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Table
p16 ARC, Monoclonal Antibody (Cat# AAA8533685)
Full Name
p16 ARC
Gene Names
ARPC5; ARC16; p16-Arc; dJ127C7.3
Reactivity
Human, Mouse, Rat
Applications
ICC, IF, IHC, IP, FC/FACS
Purity
ProA affinity purified
Pricing
$350/0.1 mL | $570/0.1 mL (Biotin) | $1,515/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Figure 1 Western Blot Validation of PD-L1 in HeLa CellsLoading: 15ug of lysates per lane. Antibodies: MBS151123 (1 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
PDL-1, Polyclonal Antibody (Cat# AAA151123)
Full Name
PDL-1 Antibody
Gene Names
CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, IHC-P, IF, FC
Purity
PD-L1 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Actinin Alpha 3, ELISA Kit (Cat# AAA26277)
Full Name
Human Actinin Alpha 3 ELISA Kit
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Testing Data
(Intracellular staining of the mouse fibroblast cell line L929 with MBS633258 (filled histogram) or with isotype control (open histogram).)
Actin, alpha, Smooth Muscle, Monoclonal Antibody (Cat# AAA633258)
Full Name
Actin, alpha, Smooth Muscle (PE)
Gene Names
ACTA2; AAT6; ACTSA; MYMY5
Reactivity
Bovine, Canine, Chicken, Frog, Goat, Guinea Pig, Human, Mouse, Rabbit, Rat, Sheep
Applications
FC/FACS
Purity
Affinity Purified
Purified by Protein G affinity chromatography
Purified by Protein G affinity chromatography
Pricing
$800/100 Tests | $3,590/5x100 Tests
Western Blot (WB)
(Western blot analysis on JK using alpha-SMA antibody, The lane on the left is blocked with the antigen-specific peptide.)
alpha-SMA, Polyclonal Antibody (Cat# AAA9600621)
Full Name
alpha-SMA Antibody
Gene Names
ACTA2; ACTSA
Reactivity
Human, Mouse, Rat, Bovine
Applications
WB, IHC, IF, ICC, IP, EIA
Purity
Peptide affinity purification
Pricing
$260/0.1 mL | $305/0.2 mL | $1,220/5x0.2 mL
Western Blot (WB)
(Western Blot analysis of CAPZA3 expression in transfected 293T cell line by CAPZA3 polyclonal antibody. Lane 1: CAPZA3 transfected lysate (32.89kD). Lane 2: Non-transfected lysate.)
CAPZA3, Polyclonal Antibody (Cat# AAA6008959)
Full Name
CAPZA3 (F-actin-capping Protein Subunit alpha-3, CapZ alpha-3, CP-alpha-3, Germ Cell-specific Protein 3, CAPAA3, GSG3)
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$620/0.05 mg | $2,775/5x0.05 mg
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (MX-49.129.5))
CD63, Monoclonal Antibody (Cat# AAA438072)
Full Name
CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody
Gene Names
CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
Reactivity
Human, Mouse
Applications
FC/FACS, IF, WB, IHC
Pricing
$390/0.1 mg (With BSA & Azide at 0.2mg/mL) | $390/0.1 mg (Without BSA & Azide at 1mg/mL) | $215/0.02 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (With BSA & Azide at 0.2mg/mL) | $1,710/5x0.1 mg (Without BSA & Azide at 1mg/mL)
Western Blot (WB)
(Western Blot analysis of CAPZA3 expression in transfected 293T cell line by CAPZA3 polyclonal antibody. Lane 1: CAPZA3 transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)
CAPZA3, Polyclonal Antibody (Cat# AAA645027)
Full Name
CAPZA3 (F-actin-capping Protein Subunit alpha-3, CapZ alpha-3, CP-alpha-3, Germ Cell-specific Protein 3, CAPAA3, GSG3)
Reactivity
Human
Applications
WB
Purity
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Pricing
$655/0.1 mg | $2,945/5x0.1 mg
SDS-Page
Probable global transcription activator SNF2L2, Recombinant Protein (Cat# AAA953002)
Full Name
Recombinant Human Probable global transcription activator SNF2L2
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $1,055/0.5 mg (Yeast) | $395/0.1 mg (E-Coli) | $440/0.1 mg (Yeast) | $245/0.02 mg (E-Coli) | $260/0.02 mg (Yeast) | $6,205/5x1 mg (E-Coli) | $4,520/5x0.5 mg (Yeast)
Testing Data
(Published customer image:hADSCs led to the appearance of perivascular spaces in between endothelial and astrocytic basement membranes one week after injection. A -D) Confocal images of horizontal sections immunostained with anti-pan-laminin antibody (red) one week after injury. Note that in DMEM animals (A,B) there is no separation between the two membranes whereas in hADSCs -treated animals (C,D) these membranes are separated (arrows in D). E) Confocal images of a horizontal section immunostained with anti-pan-laminin (green) and RECA-1(red). F -F) Confocal imagens of sequential optical sections immunostained with anti-pan-laminin (green) and DAPI (blue) showing the extravasation of cells from the blood vessels. Bars: C, F = 50 um B, D, E = 25 um.From: Menezes K, Nascimento MA, Gon§alves JP, Cruz AS, Lopes DV, et al. (2014) Human Mesenchymal Cells from Adipose Tissue Deposit Laminin and Promote Regeneration of Injured Spinal Cord in Rats. PLoS ONE 9(5): e96020.)
RECA-1, Monoclonal Antibody (Cat# AAA213213)
Full Name
MOUSE ANTI RAT RECA-1
Applications
IF
Pricing
$490/0.25 mg | $2,050/5x0.25 mg
Sequence
Granzyme M (GZMM), Active Protein (Cat# AAA2097231)
Full Name
Active Granzyme M (GZMM)
Gene Names
GZMM; MET1; LMET1
Reactivity
Homo sapiens (Human)
Applications
Cell culture; Activity Assays.
Purity
>92%
Pricing
$230/0.01 mg | $505/0.05 mg | $780/0.1 mg | $970/0.2 mg | $1,890/0.5 mg | $2,800/1 mg | $6,840/5 mg
Western Blot (WB)
(All lanes : Anti-EN1 (Engrailed 1) Antibody (N-term) at 1:2000 dilutionLane 1: U-87 MG whole cell lysateLane 2: U-2OS whole cell lysate Lane 3: SH-SY5Y whole cell lysateLane 4: 293T/17 whole cell lysateLane 5: HepG2 whole cell lysateLane 6: Mouse brain lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size: 40 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
EN1 (Engrailed 1), Polyclonal Antibody (Cat# AAA9211211)
Full Name
EN1 (Engrailed 1) Antibody (N-term)
Reactivity
Human, Mouse
Applications
WB, EIA, IHC-P, IF
Pricing
$190/0.05 mL | $425/0.2 mL | $1,665/5x0.2 mL
Western Blot (WB)
(Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)
Histone H2A.X, Monoclonal Antibody (Cat# AAA475198)
Full Name
Anti-Histone H2A.X (Phospho-Ser139) Mouse mAb
Gene Names
H2AFX; H2AX; H2A.X; H2A/X
Reactivity
Human, Mouse
Applications
WB, IF
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Immunofluorescence (IF)
(Fluorescent image of HeLa cells stained with Phospho-CDC25A(T507) Antibody. MBS9204880 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)
Phospho-CDC25A (T507), Polyclonal Antibody (Cat# AAA9204880)
Full Name
Phospho-CDC25A (T507) Antibody
Gene Names
CDC25A; CDC25A2
Reactivity
Human (Predicted Reactivity: Bovine, Mouse, Rat)
Applications
WB, EIA, IHC, IF
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$435/0.4 mL | $190/0.08 mL | $1,705/5x0.4 mL
Western Blot (WB)
(Western Blot analysis of CAPZA3 expression in transfected 293T cell line by CAPZA3 polyclonal antibody. Lane 1: CAPZA3 transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)
CAPZA3, Polyclonal Antibody (Cat# AAA648661)
Full Name
CAPZA3 (F-actin-capping Protein Subunit alpha-3, CapZ alpha-3, CP-alpha-3, Germ Cell-specific Protein 3, CAPAA3, GSG3)
Reactivity
Human
Applications
WB, IP
Purity
Serum
Serum
Serum
Pricing
$645/0.1 mL | $2,885/5x0.1 mL
Typical Testing Data/Standard Curve (for reference only)
G Actin (GACT), ELISA Kit (Cat# AAA756376)
Full Name
Human G Actin (GACT) Elisa Kit
Reactivity
Human
Pricing
$475/48-Strip-Wells | $680/96-Strip-Wells | $3,010/5x96-Strip-Wells | $5,675/10x96-Strip-Wells
Western Blot (WB)
(Figure 1 Western Blot Validation of BACELoading: 15 ug of lysates per lane. Antibodies: BACE (1ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane A-C: human brain tis)
BACE, Polyclonal Antibody (Cat# AAA150291)
Full Name
BACE Antibody
Gene Names
BACE1; ASP2; BACE; HSPC104
Reactivity
Human, Mouse
Applications
EIA, ICC, IF, IHC-P, WB
Purity
BACE Antibody is Ion exchange chromatography purified.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Smoothelin, Monoclonal Antibody (Cat# AAA570102)
Full Name
Mouse anti Smoothelin
Gene Names
SMTN; FLJ35168; FLJ35365; FLJ35620; FLJ38597
Reactivity
Canine, Chicken, Feline, Human, Monkey, Swine
Applications
IHC, frozen, IHC, paraffin, WB
Pricing
$560/0.1 mg | $2,365/5x0.1 mg
Western Blot (WB)
(Anti-KRAS Antibody (C-term) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.Predicted band size : 22 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
KRAS, Polyclonal Antibody (Cat# AAA9210256)
Full Name
KRAS Antibody (C-term)
Gene Names
KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
Reactivity
Human, Mouse, Rat
Predicted: Rat
Predicted: Rat
Applications
WB, EIA, IF, FC/FACS
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of CXCR4 in HeLa CellsLoading: 15 μg of lysates per lane. Antibodies: 1009 (1 μg/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP )
CXCR4, Polyclonal Antibody (Cat# AAA151360)
Full Name
CXCR4 Antibody
Gene Names
CXCR4; FB22; HM89; LAP3; LCR1; NPYR; WHIM; CD184; LAP-3; LESTR; NPY3R; NPYRL; HSY3RR; NPYY3R; D2S201E
Reactivity
Human, Mouse, Rat
Applications
EIA, WB, ICC, IP, IF, IHC-p, FC
Purity
CXCR4 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Testing Data
(Fig.1: FACS analysis of BOSC23 cells using GM-1E6 Cat.# MBS468007. BOSC23 cells were transiently transfected with an expression vector encoding either IL13-R (red curve) or an irrelevant protein (control transfectant: black curve). Binding of GM-1E7 was detected with a PE-conjugated secondary antibody. A positive signal was obtained only with IL13-R transfected cells.)
IL13R, Monoclonal Antibody (Cat# AAA468007)
Full Name
anti-human IL13 receptor (IL13-R) monoclonal antibody
Gene Names
IL13RA1; NR4; CD213A1; IL-13Ra
Reactivity
Human
Applications
EIA
Purity
Protein G
Pricing
$550/0.1 mg
Western Blot (WB)
(Western blot detection of E-Cadherin in T47D, HCT116, MDA-MB-468 and Caco-2 cell lysates using E-Cadherin mouse mAb (dilution 1:2000).Predicted band size:135kDa.Observed band size:135kDa.)
E-Cadherin, Monoclonal Antibody (Cat# AAA475274)
Full Name
Anti-E-Cadherin Mouse mAb
Gene Names
CDH1; UVO; CDHE; ECAD; LCAM; Arc-1; CD324
Reactivity
Human, Mouse
Applications
WB
Purity
Affinity Purified
Pricing
$440/0.1 mL | $1,530/5x0.1 mL
Activity Data
(Figure. The binding activity of EGRF2 with ACTb.The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is a transmembrane protein that is a receptor for members of the epidermal growth factor family (EGF family) of extracellular protein ligands. Epidermal growth factor receptor 2 (EGFR2) is a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. The EGFR is essential for ductal development of the mammary glands, and agonists of the EGFR such as amphiregulin, TGF-alpha, and heregulin induce both ductal and lobuloalveolar development even in the absence of estrogen and progesterone. Besides, Actin Beta (ACTb) has been identified as an interactor of EGFR2, thus a binding ELISA assay was conducted to detect the interaction of recombinant human EGFR2 and recombinant human ACTb. Briefly, EGFR2 were diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to ACTb-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EGFR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of EGFR2 and ACTb was shown in Figure 1, and this effect was in a dose dependent manner.)
Epidermal Growth Factor Receptor 2 (EGFR2), Active Protein (Cat# AAA2105153)
Full Name
Active Epidermal Growth Factor Receptor 2 (EGFR2)
Gene Names
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Applications
Cell Culture, Activity Assays
Purity
> 95%
Pricing
$220/0.01 mg | $460/0.05 mg | $705/0.1 mg | $875/0.2 mg | $1,705/0.5 mg | $2,540/1 mg | $6,205/5 mg
Western Blot (WB)
(Western blot analysis of lysates from HepG2 cell line, untreated or treated with chloroquine, using LC3 Antibody (APG8B) (N-term) (upper) or Beta-actin (lower).)
LC3, Polyclonal Antibody (Cat# AAA9213834)
Full Name
LC3 Antibody (APG8B) (N-term)
Gene Names
MAP1LC3B; LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
Reactivity
Human, rat (Predicted Reactivity: Bovine)
Applications
IF, EIA, WB, IHC
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Testing Data
(Published customer image: Mouse anti V5 tag antibody, clone SV5-Pk1 used for detection of V5 tagged REDD1 protein by western blotting.Image caption:REDD1 is not degraded by Cul4a or phosphorylated by GSK3beta at Thr 23 and Thr25. (A) HEK293 cells were transfected with empty vector or tetracycline inducible dnCul4a-V5 pcDNA4/TO (1 ug) and Cul4A-V5 pcDNA3 (0.03 ug) followed by tetracycline (1 ug/ml) induction for 24 hours and cell lysis. (B) HEK293 cells were transfected with 0.5 ug Cul4a-V5 pcDNA3 for 15 hours followed by transfection of 20 nM control or Cul4a siRNAs to determine siRNAs efficiency. (C) HEK293 cells were transfected with 20 nM control or Cul4a siRNAs for 3 days followed by cell lysis. (D) REDD1-V5 pcDNA3 wild type, T23A T25A or T23D T25D plasmids (0.4 ug) were transfected in HEK293 cells for 3 days and treated with 20 uM MG-132 for 6 hours followed by cell lysis. (E) HEK293 cells were treated with 30 mM LiCl or GSK3 inhibitor IX (5 uM or 10 uM) for 20 hours followed by cell lysis. (F) HEK293 cells were co-transfected with 0.2 ug REDD1-V5 pcDNA3 and 0.3 ug GSK3beta pcDNA3 or empty pcDNA3 for 3 days followed by MG-132 (20 uM) treatment for 6 hours followed by cell lysis. (G) HEK293 cells were transfected with 3 ug DYKDDDDK-REDD1 or DYKDDDDK-FRAT1 for 3 days followed by cell lysis and DYKDDDDK immunoprecipitation. In vitro phosphorylation of REDD1 and FRAT1 was carried out as described in Materials and Methods..From: Tan CY, Hagen T (2013) mTORC1 Dependent Regulation of REDD1 Protein Stability. PLoS ONE 8(5): e63970.)
V5-TAG, Monoclonal Antibody (Cat# AAA212109)
Full Name
MOUSE ANTI V5-TAG
Applications
EIA, FC/FACS, IF, IP, WB, RIA
Pricing
$785/1 mg | $3,370/5x1 mg
Western Blot (WB)
(Western blot analysis of Beta-actin expression in HEK293T (A), Hela (B), A375 (C), DLD (D), H460 (E), mouse kidney (F), rat kidney (G) whole cell lysates.)
Beta-actin, Polyclonal Antibody (Cat# AAA821786)
Full Name
Anti-Beta-actin Antibody, Beta-actin
Gene Names
ACTB; BRWS1; PS1TP5BP1
Reactivity
Human, Mouse, Rat, Bovine, Chicken, Dog, Monkey, Pig, Rabbit, Sheep
Applications
WB, IHC
Purity
The antibody was purified by immunogen affinity chromatography.
Pricing
$155/0.03 mL | $210/0.1 mL | $275/0.2 mL | $1,175/5x0.2 mL
Testing Data
beta-Actin, Polyclonal Antibody (Cat# AAA448085)
Full Name
Anti-beta-Actin
Reactivity
Human, Rat, Mouse, Monkey, and Canine proteins
Applications
WB, IF
Pricing
$320/0.6 mg | $1,430/5x0.6 mg
Immunofluorescence (IF)
(Confocal immunofluorescent analysis of MUSK Antibody (MBS9205728) with MDA-MB231 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)
MUSK, Polyclonal Antibody (Cat# AAA9205728)
Full Name
MUSK Antibody
Gene Names
MUSK; CMS9
Reactivity
Human, mouse
Applications
WB, EIA, FC, IF
Purity
Purified Rabbit Polyclonal Antibody (PAB)
Pricing
$405/0.4 mL | $185/0.08 mL | $1,585/5x0.4 mL
alpha Smooth Muscle Actin, ELISA Kit (Cat# AAA43485)
Full Name
Human alpha Smooth Muscle Actin ELISA Kit
Gene Names
ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
Reactivity
Human
Pricing
$540/48-Strip-Wells | $755/96-Strip-Wells | $3,410/5x96-Strip-Wells | $6,715/10x96-Strip-Wells
Immunofluorescence (IF)
(Fluorescent image of A549 cell stained with PCM-1 Antibody (Center). A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with PCM-1 primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).PCM-1 immunoreactivity is localized to Centrosome significantly.)
PCM-1, Polyclonal Antibody (Cat# AAA9209983)
Full Name
PCM-1 Antibody (Center)
Gene Names
PCM1; PTC4; RET/PCM-1
Reactivity
Human
Applications
WB, EIA, IHC, FC/FACS, IF
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
$415/0.4 mL | $185/0.08 mL | $1,625/5x0.4 mL
Western Blot (WB)
(Figure 1 Western Blot Validation of MyD88 in HeLa (A) and Jurket (B) CellsLoading: 15 μg of lysates per lane.Antibodies: 2125 (1 μg/mL) 1 h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.)
MYD88, Polyclonal Antibody (Cat# AAA150754)
Full Name
MYD88 Antibody
Gene Names
MYD88; MYD88D
Reactivity
Human, Mouse
Applications
EIA, WB, IHC, IF
Purity
MYD88 Antibody is affinity chromatography purified via peptide column.
Pricing
$440/0.1 mg | $1,960/5x0.1 mg
Immunofluorescence
(Immunofluorescent staining using Western Blot analysis using Nucleophosmin antibodyConfocal immunofluorescence analysis of Hela (left) and NTERA-2 (right) cells using NPM antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.)
Nucleophosmin, Monoclonal Antibody (Cat# AAA830822)
Full Name
Nucleophosmin antibody
Gene Names
NPM1; B23; NPM
Applications
EIA, IF, IHC, WB
Pricing
$525/0.1 mL | $2,355/5x0.1 mL
