Mouse anti-Human, Mouse CD63 Monoclonal Antibody | anti-CD63 antibody

CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody

Cat. Num. AAA438072

• Gene Names: CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• Reactivity: Human, Mouse
• Applications: Flow Cytometry, Functional Assay, Immunofluorescence, Western Blot, Immunohistochemistry
• Synonyms: CD63; Monoclonal Antibody; CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody; gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30; anti-CD63 antibody

• Host: Mouse
• Reactivity: Human, Mouse
• Clonality: Monoclonal
• Isotype: IgG1, kappa
• Clone Number: MX-49.129.5
• Specificity: This MAb recognizes protein of 26kDa-60kDa, which is identified as CD63. Its epitope is different from that of MAb LAMP3/529. The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.
• Form/Format: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
• Sequence Length: 238

• Applicable Applications for anti-CD63 antibody: Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin
• Application Notes: Flow Cytometry (0.5-1ug/million cells in 0.1ml); Immunofluorescence (0.5-1ug/ml); Western Blot (0.5-1ug/ml); Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
• Cellular Localization: Cell Surface & Cytoplasmic
• Immunogen: Full length CD63 of human origin
• Hu-Chromosome Location: 12q13.2
• Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.
• Preparation and Storage: Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (MX-49.129.5))

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded mouse spleen stained with CD63 Monoclonal Antibody (MX-49.129.5))

Western Blot (WB)

(Western Blot of human Spleen Lysate with CD63 Monoclonal Antibody (MX-49.129.5))

Flow Cytometry (FC/FACS

(Flow Cytometry of NIH/3T3 Cells.Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (MX-49.129.5).)

Immunofluorescence (IF)

(IF staining of HeLa cells using AF488 labeled CD63 Monoclonal Antibody (MX-49.129.5) (green). F-actin filaments are labeled with Dylight 554 phalloidin (red). Nuclei stained with DAPI (blue).)

Immunohistochemistry (IHC)

(Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with CD63 Monoclonal Antibody (MX-49.129.5))

References

C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985. AA Palmer et al., Pathology 17: 335-339, 1985. EC Hagen et al., Histopathology 10: 689-700, 1986.

NCBI and Uniprot Product Information

• NCBI GI #: 383872447
• NCBI GeneID: 967
• NCBI Accession #: NP_001244318.1
• NCBI GenBank Nucleotide #: NM_001257389.1
• UniProt Accession #: P08962; Q5TZP3; Q8N6Z9; Q9UCG6; F8VZE2
• Molecular Weight: 26kDa (core protein); 30-60kDa (glycosylated)
• NCBI Official Full Name: CD63 antigen isoform A
• NCBI Official Synonym Full Names: CD63 molecule
• NCBI Official Symbol: CD63
• NCBI Official Synonym Symbols: MLA1; ME491; LAMP-3; OMA81H; TSPAN30
• NCBI Protein Information: CD63 antigen
• UniProt Protein Name: CD63 antigen
• Protein Family: CD63 antigen
• UniProt Gene Name: CD63
• UniProt Synonym Gene Names: MLA1; TSPAN30; LAMP-3; Tspan-30
• UniProt Entry Name: CD63_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

Uniprot Description

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q12-q13

Cellular Component: cell surface; endosome membrane; extracellular space; integral to plasma membrane; intrinsic to plasma membrane; late endosome membrane; lysosomal membrane; plasma membrane; platelet dense granule membrane

Molecular Function: protein binding

Biological Process: cell migration; cell-matrix adhesion; pigment granule maturation; platelet degranulation; positive regulation of receptor internalization