Sequence
Granzyme M (GZMM) Active Protein | GZMM active protein
Active Granzyme M (GZMM)
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Sequence
Sequence
Activity Data
(GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets alpha-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs beta-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin.)
Activity Data
(GZMM (Granzyme M) is one of the neutral serine proteases, which is specifically expressed by NK cells and mediates a novel major and perforin-dependent cell death pathway. Granzyme M has been proven to targets alpha-Tubulin and disorganizes the microtubule network, besides, Ezrin has also been identified as a substrate of GZMM. Therefore, a catalytic assay was conducted to detect the protease activity of recombinant human GZMM using Hela cells lysates. Briefly, protein lysates were extracted from 2×107 Hela cells using Lysis Buffer, then incubated with normal or inactivated GZMM in 37oC for 4h. Samples were immunoblotted using Abs beta-actin as control, and Ezrin to detect the enzyme activity. The results were shown below. It is obvious that recombinant human GZMM cleaved Ezrin.)
Gene Sequencing (extract)
Gene Sequencing (extract)
SDS-PAGE
(SDS-PAGESample: Active recombinant GZMM, Human)
SDS-PAGE
(SDS-PAGESample: Active recombinant GZMM, Human)
Western Blot (WB)
(Western BlotSample: Recombinant GZMM, Human;Antibody: Rabbit Anti-Human GZMM Ab (Catalog #: MBS2004112))
Western Blot (WB)
(Western BlotSample: Recombinant GZMM, Human;Antibody: Rabbit Anti-Human GZMM Ab (Catalog #: MBS2004112))
NCBI and Uniprot Product Information
Accurate Molecular Mass: 26kDa as determined by SDS-PAGE reducing conditions
NCBI Description
Human natural killer (NK) cells and activated lymphocytes express and store a distinct subset of neutral serine proteases together with proteoglycans and other immune effector molecules in large cytoplasmic granules. These serine proteases are collectively termed granzymes and include 4 distinct gene products: granzyme A, granzyme B, granzyme H, and the protein encoded by this gene, granzyme M. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]
Uniprot Description
GZMM: Cleaves peptide substrates after methionine, leucine, and norleucine. Physiological substrates include EZR, alpha- tubulins and the apoptosis inhibitor BIRC5/Survivin. Promotes caspase activation and subsequent apoptosis of target cells. Belongs to the peptidase S1 family. Granzyme subfamily.
Protein type: EC 3.4.21.-; Protease; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 19p13.3
Cellular Component: extracellular region; membrane
Molecular Function: protein binding; serine-type endopeptidase activity; serine-type peptidase activity
Biological Process: apoptosis; cell death; cytolysis; innate immune response; proteolysis
Research Articles on GZMM
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Product Notes
The GZMM gzmm (Catalog #AAA2097231) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Granzyme M (GZMM) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Granzyme M (GZMM) can be used in a range of immunoassay formats including, but not limited to, Cell culture; Activity Assays. (May be suitable for use in other assays to be determined by the end user.). Researchers should empirically determine the suitability of the GZMM gzmm for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Granzyme M (GZMM), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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