Human Gelsolin (GS) ELISA Kit | GS elisa kit

Human Gelsolin (GS) ELISA Kit

Cat. Num. AAA264815

• Reactivity: Human
• Synonyms: Gelsolin (GS); Human Gelsolin (GS) ELISA Kit; GS elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 780

• Samples: Human serum, plasma or Cell Culture Supernatant and organizations in the natural and recombinant GS concentration
• Assay Type: Sandwich
• Detection Range: 100 ng/ml-1.56 ng/ml
• Sensitivity: 0.5 ng/ml.
• Intra-assay Precision: <= 8%
• Inter-assay: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for GS elisa kit

Principle of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human GS monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 51854227
• NCBI GeneID: 296654
• NCBI Accession #: NP_001004080.1
• NCBI GenBank Nucleotide #: NM_001004080.1
• Molecular Weight: 80,929 Da
• NCBI Official Full Name: gelsolin
• NCBI Official Synonym Full Names: gelsolin
• NCBI Official Symbol: Gsn
• NCBI Protein Information: gelsolin; ADF; brevin; actin-depolymerizing factor
• UniProt Protein Name: Gelsolin
• UniProt Gene Name: Gsn
• UniProt Synonym Gene Names: ADF
• UniProt Entry Name: GELS_RAT

NCBI Description

plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; may regulate sperm release and turnover of the blood-testis barrier [RGD, Feb 2006]

Uniprot Description

Gelsolin: a calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Its expression is significantly decreased in many tumors and transformed cell lines. Gelsolin and other proteins including E-cadherin, BRCA, p21WAF, HoxA5, and retinoic acid receptor II are regulated by epigenetic changes in breast cancers. Gelsolin helps mediate the ability of HDAC inhibitors to protect neurons from oxygen/glucose deprivation. Two human isoforms are produced by alternative initiation.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted; Nuclear receptor co-regulator

Cellular Component: ruffle; extracellular space; focal adhesion; protein complex; perinuclear region of cytoplasm; lamellipodium; extracellular region; cytosol; actin cytoskeleton

Molecular Function: calcium ion binding; actin binding

Biological Process: regulation of cell adhesion; actin filament severing; phosphoinositide-mediated signaling; wound healing; apoptosis; actin nucleation; vesicle-mediated transport; response to ethanol; actin filament polymerization; tissue regeneration; actin polymerization and/or depolymerization; response to folic acid; oligodendrocyte development; barbed-end actin filament capping; aging