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Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Mouse Smoothelin Monoclonal Antibody | anti-SMTN antibody
Mouse anti Smoothelin
Gene Names
SMTN; FLJ35168; FLJ35365; FLJ35620; FLJ38597
Reactivity
Canine, Chicken, Feline, Human, Monkey, Swine
Applications
Immunohistochemistry, Immunohistochemistry, Western Blot
Synonyms
Smoothelin; Monoclonal Antibody; Mouse anti Smoothelin; anti-SMTN antibody
Host
Mouse
Reactivity
Canine, Chicken, Feline, Human, Monkey, Swine
Clonality
Monoclonal
Isotype
IgG1
Clone Number
R4A
Specificity
R4A reacts with the 59 kDa and 100 kDa protein, corresponding to smoothelin A and B, respectively, which are exclusively found in smooth muscle cells.
Form/Format
Each vial contains 100 ul 1 mg/ml purified monoclonal antibody in PBS containing 0.09% sodium azide.
Applicable Applications for anti-SMTN antibody
Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB)
Application Notes
R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citRate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations.
Source Note
R4A is a Mouse monoclonal IgG1 antibody derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a Mouse immunized with a cytoskeletal extract of Chicken gizzard.
Preparation and Storage
Store at 4 degree C, or in small aliquots at -20 degree C.
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Immunohistochemistry (IHC)
(Figure 1. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Immunohistochemistry (IHC)
(Figure 2. Immunohistochemistry of R4A on frozen section of chicken gizzard striated muscle.)
Immunohistochemistry (IHC)
(Figure 3. Immunohistochemistry of R4A on paraffin section of human smooth muscle.)
Immunohistochemistry (IHC)
(Figure 3. Immunohistochemistry of R4A on paraffin section of human smooth muscle.)
Immunohistochemistry (IHC)
(Figure 4. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunohistochemistry (IHC)
(Figure 4. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunohistochemistry (IHC)
(Figure 5. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunohistochemistry (IHC)
(Figure 5. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunohistochemistry (IHC)
(Figure 6. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunohistochemistry (IHC)
(Figure 6. Smooth muscle cells in swine colon as detected (R4A; dilution 1:100).)
Immunofluorescence (IF)
(Figure 7. Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by R4A) and actin (green fluorescence).)
Immunofluorescence (IF)
(Figure 7. Indirect immunofluorescence staining of a cultured human fibroblast, showing colocalization of smoothelin (red fluorescence; as detected by R4A) and actin (green fluorescence).)
Immunoblot (IB)
(Figure 8. Immunoblotting results (R4A) on uterine artery and uterine corpus.)
Immunoblot (IB)
(Figure 8. Immunoblotting results (R4A) on uterine artery and uterine corpus.)
Related Product Information for anti-SMTN antibody
Smoothelin is a constituent of the smooth muscle cell (SMC) cytoskeleton. Antibodies directed to smoothelin are useful tools to monitor SMC differentiation. Smoothelin is exclusively expressed in fully differentiated (contractile) SMCs. RNA and protein analyses revealed a broad species distribution of this protein. Cells with SMC-like characteristics, such as myofibroblasts and myoepithelial cells, as well as skeletal and cardiac muscle do not contain smoothelin. Confocal scanning laser microscopy of tissue sections and cells in culture show a filamentous organization of smoothelin colocalizing with actin stress fibers. In immunoblots two molecular weight isoforms are detected i.e. a 59 kDa isoform specific for visceral SMC (smoothelin A), and an isoform with a molecular weight of approximately 100 kDa in vascular SMC (smoothelin B). Human smoothelin is encoded by a single copy gene which is loCated on chromosome 22.
Product Categories/Family for anti-SMTN antibody
References
van der Loop, F. T., Schaart, G., Timmer, E. D., Ramaekers, F. C., and van Eys, G. J. (1996). Smoothelin, a novel cytoskeletal protein specific for smooth muscle cells, J Cell Biol 134, 401-11. Wehrens, X. H., Mies, B., Gimona, M., Ramaekers, F. C., Van Eys, G. J., and Small, J. V. (1997). Localization of smoothelin in avian smooth muscle and identifiCation of a vascular-specific isoform, FEBS Lett 405, 315-20. van der Loop, F. T., Gabbiani, G., Kohnen, G., Ramaekers, F. C., and van Eys, G. J. (1997). Differentiation of smooth muscle cells in Human blood vessels as defined by smoothelin, a novel marker for the contractile phenotype, Arterioscler Thromb Vasc Biol 17, 665-71. Christen, T., Bochaton-Piallat, M. L., Neuville, P., Rensen, S., Redard, M., van Eys, G., and Gabbiani, G. (1999). Cultured porcine coronary artery smooth muscle cells. A new model with advanced differentiation, Circ Res 85, 99-107. Johansson, B., Eriksson, A., Ramaekers, F., and Thornell, L. (1999). Smoothelin in adult and developing Human arteries and myocardium, Histochem Cell Biol 112, 291-9. Johansson, B., Eriksson, A., Ramaekers, F., and Thornell, L. E. (1999). Smoothelin and intermediate filament proteins in Human aortocoronary saphenous vein by-pass grafts, Histochem J 31, 723-7. Deruiter, M. C., Rensen, S. S., Coolen, G. P., Hierck, B. P., Bergwerff, M., Debie, W. M., Gittenberger-De Groot, A. C., and Van Eys, G. J. (2001). Smoothelin expression during Chicken embryogenesis: detection of an embryonic isoform, Dev Dyn 221, 460-3. Council, L., Hameed, O. (2009). Differential expression of immunohistochemical markers in bladder smooth muscle and myofibroblasts, and the potential utility of desmin, smoothelin, and vimentin in staging of bladder carcinoma Modern Pathology 22, 639-50. Coco, D.P., Hirsch, M.S., Hornick, J.L. (2009). Smoothelin is a specific marker for smooth muscle neoplasms of the gastrointestinal tract. Am J Surg Pathol 33, 1795-1801.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
99,059 Da
NCBI Official Full Name
Smoothelin
NCBI Official Synonym Full Names
smoothelin
NCBI Official Symbol
SMTN
NCBI Official Synonym Symbols
FLJ35168; FLJ35365; FLJ35620; FLJ38597
NCBI Protein Information
smoothelin; OTTHUMP00000199381; OTTHUMP00000199382; OTTHUMP00000199383
UniProt Protein Name
Smoothelin
Protein Family
UniProt Gene Name
SMTN
UniProt Synonym Gene Names
SMSMO
UniProt Entry Name
SMTN_HUMAN
NCBI Description
This gene encodes a structural protein that is found exclusively in contractile smooth muscle cells. It associates with stress fibers and constitutes part of the cytoskeleton. This gene is localized to chromosome 22q12.3, distal to the TUPLE1 locus and outside the DiGeorge syndrome deletion. Alternative splicing of this gene results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq]
Uniprot Description
smoothelin: Structural protein of the cytoskeleton. Belongs to the smoothelin family. 4 isoforms of the human protein are produced by alternative promoter.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 22q12.2
Cellular Component: nucleoplasm; cytoskeleton; cytoplasm; actin cytoskeleton
Molecular Function: structural constituent of muscle; actin binding
Biological Process: muscle development; smooth muscle contraction
Research Articles on SMTN
Similar Products
Product Notes
The SMTN smtn (Catalog #AAA570102) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Smoothelin reacts with Canine, Chicken, Feline, Human, Monkey, Swine and may cross-react with other species as described in the data sheet. AAA Biotech's Smoothelin can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) (frozen), Immunohistochemistry (IHC) (paraffin), Western Blot (WB). R4A is suitable for immunoblotting and immunohistochemistry on frozen and paraffin-embedded tissues. For staining paraffin-embedded tissues pretreatment in 10 mM citRate buffer (pH 6.0) and heating for 3 times 5 minutes in a microwave is required. Optimal antibody dilution should be determined by titRation; recommended range is 1:100 - 1:200 for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting appliCations. Researchers should empirically determine the suitability of the SMTN smtn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Smoothelin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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