Actin (N-terminal Region) Peptide

Synonyms

Actin (N-terminal Region) Peptide; Actin (N-terminal Region) Peptide peptide

Ordering
Cat. Num. AAA474001 For Research Use Only!

Specificity

The peptide is specifically recognized by actin (Nterminal Region) antibody (AP1651) in ELISA, and has been shown to block the reactivity of AP1651 in Western blot and immunocytochemistry.

Form/Format

Blocking Peptide is supplied in 50ul phosphate-buffered saline and 0.05% sodium azide.

Sequence

Peptide Sequence: Actin synthetic peptide (coupled to KLH) corresponding to amino acid residues in the N-terminal region of human beta actin. This sequence is identical to similar regions in all four a actins, as well as in gamma actin, and is well conserved in actins from most eukaryotic species.

Application Notes

Blocking: 1:1,000
ELISA: 50 ng/well

Expression Region

Amino acids 49-62 from Human Beta-Actin

Preparation and Storage

Store at -20 degree C. Stable for 1 year.

Related Product Information for Actin (N-terminal Region) Peptide peptide

Actin is a major cytoskeletal protein involved in diverse cellular functions including cell motility, adhesion, and morphology. Six different actin isoforms have been identified in vertebrates. There are four a isoforms: skeletal, cardiac, and two smooth muscle (enteric and aortic) actins, along with two cytoplasmic actins (beta and gamma). Actin exists in two principal forms, globular, monomeric (G) actin, and filamentous polymeric (F) actin. The assembly and disassembly of actin filaments, and also their organisation into functional networks, is regulated by a variety of actin-binding proteins (ABPs). Phosphorylation may also be important for regulating actin assembly and interaction with ABPs. In Dictyostelium, phosphorylation of Tyr-53 occurs in response to cell stress and this phosphorylation may alter actin polymerizaiton. In B cells, SHP-1 tyrosine dephosphorylation of actin leads to actin filament depolymerization following BCR stimulation.

References

• Baba, T. et al. (2003) J. Immunol. 170: 3762.
• Jungbluth, A. et al. (1995) FEBS Let. 375:87.
• Winder, S.J. et al. (2005) J. Cell Sci. 118:651.
• Liu, X. et al. (2006) Proc Nat Acad Sci U S A. 103(37):13694

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Product Notes

The Actin (N-terminal Region) Peptide (Catalog #AAA474001) is a Peptide and is intended for research purposes only. The product is available for immediate purchase. Blocking: 1:1,000 ELISA: 50 ng/well. Researchers should empirically determine the suitability of the Actin (N-terminal Region) Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Peptide Sequence: Actin synthetic peptide (coupled to KLH) correspond ing to amino acid residues in the N-terminal region of human beta actin. This sequence is identical to similar regions in all four an actins, as well as in gamma actin, and is well conserved in actins from most eukaryotic species. It is sometimes possible for the material contained within the vial of "Actin (N-terminal Region) Peptide, Peptide" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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Actin (N-terminal Region) Peptide