Alpha Actinin Native Protein | ACTN1 native protein
Alpha Actinin
Purity
Greater than 95.0% as determined by analysis by SDS-PAGE.
Synonyms
Alpha Actinin; Alpha-actinin-1; Alpha-actinin cytoskeletal isoform; Non-muscle alpha-actinin-1; F-actin cross-linking protein; ACTN1.; ACTN1 native protein
Host
Chicken Gizzard
Purity/Purification
Greater than 95.0% as determined by analysis by SDS-PAGE.
Form/Format
The protein was lyophilized from a 1mg/ml solution containing 10mM Tris acetate buffer pH 7.6, 0.1mM EDTA, 2mM DTT, and 20mM NaCl.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
Sequence Length
888
Application Notes
Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization.
Solubility
It is recommended to reconstitute the lyophilized a-Actinin in sterile 18M Omega -cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.
Preparation and Storage
Lyophilized a-Actinin although stable at room temperature for 3 weeks, should be stored desiccated below -18 degree C. Upon reconstitution a-Actinin should be stored at 4 degree C between 2-7 days and for future use below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Related Product Information for ACTN1 native protein
Description: Ultra pure Alpha Actinin having a Molecular mass of 95,000 Dalton.
Introduction: ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Introduction: ACTN1 encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments.
Product Categories/Family for ACTN1 native protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
102,855 Da
NCBI Official Full Name
alpha-actinin-1
NCBI Official Synonym Full Names
actinin, alpha 1
NCBI Official Symbol
ACTN1
NCBI Protein Information
alpha-actinin-1; F-actin cross-linking protein; alpha-actinin cytoskeletal isoform; non-muscle alpha-actinin-1
UniProt Protein Name
Alpha-actinin-1
Protein Family
UniProt Gene Name
ACTN1
UniProt Entry Name
ACTN1_CHICK
Uniprot Description
F-actin cross-linking protein is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
Research Articles on ACTN1
Similar Products
Product Notes
The ACTN1 actn1 (Catalog #AAA143070) is a Native Protein produced from Chicken Gizzard and is intended for research purposes only. The product is available for immediate purchase. Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization. Researchers should empirically determine the suitability of the ACTN1 actn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Alpha Actinin, Native Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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