Rabbit Bmf (NT) Polyclonal Antibody
Bmf (NT) Polyclonal Antibody
Applications
Immunoblot, Western Blot
Synonyms
Bmf (NT); Polyclonal Antibody; Bmf (NT) Polyclonal Antibody; anti-Bmf (NT) antibody
Host
Rabbit
Clonality
Polyclonal
Specificity
Bmf (NT) [This rabbit antibody recognizes an epitope of aa 2-14 at the N-terminus of human Bmf (Bcl-2-Modifying Factor), a novel BH3-only protein.]
This antibody recognizes an epitope at the N-terminus of human Bmf (Bcl-2- Modifying Factor), a novel BH3-only protein. In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiaing the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
This antibody recognizes an epitope at the N-terminus of human Bmf (Bcl-2- Modifying Factor), a novel BH3-only protein. In healthy cells, Bmf associates with the dynein light chain 2 component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiaing the downstream apoptotic program. Bmf is constitutively expressed in many tissues.
Form/Format
100 ug IgG in PBS, pH 7.4, purified by immunoaffinity chromatography.
Applicable Applications for anti-Bmf (NT) antibody
Immunoblot
Application Notes
Immunoblotting: use at 2 ug/ml. In immunoblots, a band of 25 kD is detected.
Positive control: HepG2 or 293 cell lysates.
Positive control: HepG2 or 293 cell lysates.
Antigen
Peptide corresponding to aa 2-14 of human Bmf.
Stabilizer
None
Preservatives
0.02% sodium azide
Dilutions Instructions
Dilute in PBS or medium which is identical to that used in the assay system.
Preparation and Storage
This antibody is stable for at least one (1) year at -20 degree C. Avoid multiple freeze-thaw cycles.
Product Categories/Family for anti-Bmf (NT) antibody
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Product Notes
The Bmf (NT) (Catalog #AAA194181) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Bmf (NT) can be used in a range of immunoassay formats including, but not limited to, Immunoblot. Immunoblotting: use at 2 ug/ml. In immunoblots, a band of 25 kD is detected. Positive control: HepG2 or 293 cell lysates. Researchers should empirically determine the suitability of the Bmf (NT) for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Bmf (NT), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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