Rat TNFSF11/RANKL ELISA Kit | TNFSF11 elisa kit

Rat TNFSF11/RANKL PicoKine ELISA Kit

Cat. Num. AAA178747

• Gene Names: Tnfsf11; RANKL
• Reactivity: Rat
• Synonyms: TNFSF11/RANKL; Rat TNFSF11/RANKL PicoKine ELISA Kit; CD254; O14788; ODF; OPGL; RANKL; TRANCE; OPTB2; Osteoprotegerin ligand; sOdf; TNF11; TNFSF11; Tumor necrosis factor ligand superfamily member 11; TNFSF11 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat TNFSF11
• Sequence Length: 318

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 15-20
• Immunogen: E Coli, F141-D318
• Sample Data: Concentration(pg/ml): |0|15.6|31.2|62.5|125|250|500|1000; O.D.: |0.087|0.145|0.202|0.315|0.522|0.909|1.581|2.347
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |33.4|156|526|45.8|188|641; Standard Deviation: |1.50|7.64|29.46|2.29|12.41|47.43; CV(%): |4.5|4.9|5.6|5.0|6.6|7.4
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml rat TNFSF11 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each rat TNFSF11 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat TNFSF11/RANKL PicoKine ELISA Kit standard curve )

Related Product Information for TNFSF11 elisa kit

For quantitative detection of rat TNFSF11 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. The rat gene is mapped to 15q11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.
Principle: Rat TNFSF11 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for TNFSF11 has been precoated onto 96-well plates. Standards (Expression system for standard: E Coli, Immunogen sequence: F141-D318) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFSF11 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat TNFSF11 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 16924012
• NCBI GeneID: 117516
• NCBI Accession #: NP_476490.1
• NCBI GenBank Nucleotide #: NM_057149.1
• UniProt Accession #: Q9ESE2; Q91ZI9
• Molecular Weight: 35,370 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE

NCBI Description

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.