Mouse Meteorin-like/METRNL ELISA Kit | METRNL elisa kit

Mouse Meteorin-like/METRNL PicoKine ELISA Kit

Cat. Num. AAA178740

• Gene Names: Metrnl; BC019776; 9430048M07Rik
• Reactivity: Mouse
• Synonyms: Meteorin-like/METRNL; Mouse Meteorin-like/METRNL PicoKine ELISA Kit; Meteorin-like protein; Metrnl; Subfatin; Q8VE43; meteorin; glial cell differentiation regulator-like; METRNL elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Meteorin-like
• Sequence Length: 311

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 15-20
• Immunogen: NSO, Q46-E311
• Sample Data: Concentration(pg/ml): |0|62.5|125|250|500|1000|2000|4000; O.D.: |0.059|0.156|0.251|0.387|0.755|1.286|1.910|2.347
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |132|600|2011|154|713|2587; Standard Deviation: |6.07|29.40|106.58|8.93|47.77|191.44; CV(%): |4.6|4.9|5.3|5.8|6.7|7.4
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Meteorin-like standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Meteorin-like standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse Meteorin-like/METRNL PicoKine ELISA Kit standard curve )

Related Product Information for METRNL elisa kit

For quantitative detection of mouse Meteorin-like in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: METRNL (Meteorin-like) is a cytoplasmic 30 kDa (predicted) member of the meteorin family of proteins. It is expressed by neurons, and appears to function as a primer molecule that sets the stage for either cell differentiation or neurite outgrowth when it acts with Dclk1 and SerpinB1. In mice, exogenously elevated circulating Metrnl increased whole-body energy expenditure and improved glucose tolerance following diet-induced obesity. It also induced expression of antiinflammatory genes, leading to alternative activation of macrophages in adipose tissue and to browning of white fat depots. Metrnl did not appear to activate thermogenic genes directly, but it stimulated eosinophils and macrophages to enter adipose tissue and take their specific thermogenic actions. Blockade of cytokine signaling eliminated Metrnl-induced changes in gene expression required for alternative macrophage activation and blunted expression of beige fat thermogenic and beta-oxidation genes. It is concluded that METRNL is a hormone that influences energy expenditure and thermogenic programs in adipose tissue.
Principle: Mouse Meteorin-like ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for Meteorin-like has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q46-E311) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin-like is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin-like amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 21450153
• NCBI GeneID: 210029
• NCBI Accession #: NP_659046.1
• NCBI GenBank Nucleotide #: NM_144797.3
• UniProt Accession #: Q8VE43; Q8R1J2
• Molecular Weight: 25,721 Da
• NCBI Official Full Name: meteorin-like protein
• NCBI Official Synonym Full Names: meteorin, glial cell differentiation regulator-like
• NCBI Official Symbol: Metrnl
• NCBI Official Synonym Symbols: BC019776; 9430048M07Rik
• NCBI Protein Information: meteorin-like protein
• UniProt Protein Name: Meteorin-like protein
• Protein Family: Meteorin-like protein
• UniProt Gene Name: Metrnl

Uniprot Description

METRNL: Belongs to the meteorin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 11|11 E2

Cellular Component: extracellular space

Molecular Function: hormone activity

Biological Process: brown fat cell differentiation; fat cell differentiation; negative regulation of inflammatory response; response to cold; response to muscle activity