Mouse Oncostatin M/OSM PicoKine ELISA Kit | Osm elisa kit

Mouse Oncostatin M/OSM PicoKine ELISA Kit

Cat. Num. AAA177594

• Gene Names: Osm; OncoM
• Reactivity: Mouse
• Synonyms: Oncostatin M/OSM PicoKine; Mouse Oncostatin M/OSM PicoKine ELISA Kit; Oncostatin-M; MGC20461; ONCM_HUMAN; Oncostatin M precursor; OncostatinM; OSM; OTTMUSP00000005249; RP23-453B22.1; oncostatin M; Osm elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse Oncostatin M
• Sequence Length: 263

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse Oncostatin M in cell culture supernates, serum and plasma(heparin, EDTA).
• Immunogen: E Coli, A24-R206
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse Oncostatin M standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Oncostatin M standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|113|468|1221|126|471|1288; Standard deviation|4.86|22.00|69.60|10.46|41.92|117.21; CV(%)|4.3|4.7|5.7|8.3|8.9|9.1
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.031|0.074|0.157|0.305|0.587|1.032|1.901|2.369
• Anticoagulant: Heparin or EDTA
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse Oncostatin M/OSM PicoKine ELISA Kit standard curve )

Related Product Information for Osm elisa kit

Principle of the assay: Mouse Oncostatin M ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for Oncostatin M has been precoated onto 96-well plates. Standards(E Coli, A24-R206) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Oncostatin M is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse Oncostatin M amount of sample captured in plate.

Background: OSM(Oncostatin M) is a protein that in humans is encoded by the OSM gene. OSM is a pleiotropic cytokine that belongs to the interleukin 6 group of cytokines. The OSM gene is mapped on 22q12.2. Of these cytokines it most closely resembles leukemia inhibitory factor(LIF) in both structure and function. Treatment with recombinant OSM leads to the inhibition of proliferation and changes in cellular morphology of a number of tumor cell lines derived from a wide variety of tissue types. OSM signals through cell surface receptors that contain the protein gp130. The type I receptor is composed of gp130 and LIFR, the type II receptor is composed of gp130 and OSMR.

NCBI and Uniprot Product Information

• NCBI GI #: 61656173
• NCBI GeneID: 18413
• NCBI Accession #: NP_001013383.1
• NCBI GenBank Nucleotide #: NM_001013365.2
• UniProt Accession #: P53347; Q3U1Y5; Q5SPX6
• Molecular Weight: 30,114 Da
• NCBI Official Full Name: oncostatin-M
• NCBI Official Synonym Full Names: oncostatin M
• NCBI Official Symbol: Osm
• NCBI Official Synonym Symbols: OncoM
• NCBI Protein Information: oncostatin-M
• UniProt Protein Name: Oncostatin-M
• Protein Family: Oncostatin
• UniProt Gene Name: Osm
• UniProt Synonym Gene Names: OSM
• UniProt Entry Name: ONCM_MOUSE

Uniprot Description

OSM: Growth regulator. Inhibits the proliferation of a number of tumor cell lines. Stimulates proliferation of AIDS-KS cells. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells. Uses both type I OSM receptor (heterodimers composed of LIPR and IL6ST) and type II OSM receptor (heterodimers composed of OSMR and IL6ST). Involved in the maturation of fetal hepatocytes, thereby promoting liver development and regeneration. Belongs to the LIF/OSM family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Cellular Component: extracellular region; extracellular space

Molecular Function: cytokine activity; growth factor activity; oncostatin-M receptor binding

Biological Process: behavioral response to pain; immune response; negative regulation of cell proliferation; negative regulation of hormone secretion; negative regulation of meiosis; peripheral nervous system development; positive regulation of cell proliferation; positive regulation of MAPKKK cascade; positive regulation of peptidyl-serine phosphorylation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; regulation of growth; response to heat; tyrosine phosphorylation of Stat1 protein; tyrosine phosphorylation of Stat3 protein; tyrosine phosphorylation of Stat5 protein