Mouse TNFR1 PicoKine ELISA Kit | Tnfrsf1a elisa kit

Mouse TNFR1 PicoKine ELISA Kit

Cat. Num. AAA177641

• Gene Names: Tnfrsf1a; FPF; p55; TNF-R; TNFAR; TNFRI; Tnfr1; p55-R; CD120a; TNF-R1; TNFR60; Tnfr-2; TNF-R-I; TNF-R55; TNFRp55; TNF-alphaR1; TNFalpha-R1
• Reactivity: Mouse
• Synonyms: TNFR1 PicoKine; Mouse TNFR1 PicoKine ELISA Kit; Tumor necrosis factor receptor superfamily member 1A; CD120a; FPF; p55; p55 R; p60; p65; TBP1; TBPI; TNF R; TNF R I; TNF R55; TNF-R1; TNF-RI; TNFAR; TNFR I; TNFR1; TNFR55; TNFR60; TNFRI; TNFRSF1A; TNR1A_HUMAN; Tumor necrosis factor alpha receptor; Tumor necrosis factor binding protein 1; Tumor necrosis factor receptor 1; membrane form; Tumor necrosis factor receptor type I; Tumor necrosis factor-binding protein 1; tumor necrosis factor receptor superfamily; member 1A; Tnfrsf1a elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse TNFR1
• Sequence Length: 454

• Assay Range: 15.6pg/ml-1000pg/ml
• Sensitivity: <10pg/ml
• Sample: For quantitative detection of mouse TNFR1 in cell culture supernates and serum.
• Immunogen: NSO, L30-A212
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse TNFR1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse TNFR1 standard solution and each sample be measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|46|118|581|48.3|120|592; Standard deviation|2.7|6.5|27.5|2.5|6|33.3; CV(%)|5.9|5.5|4.7|5.2|5|5.6
• Sample Data: Concentration(pg/ml)|0|15.6|31.2|62.5|125|250|500|1000; O.D.|0.036|0.110|0.215|0.442|0.822|1.328|1.876|2.152
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse TNFR1 PicoKine ELISA Kit standard curve )

Related Product Information for Tnfrsf1a elisa kit

Principle of the assay: Mouse TNFR1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for TNFR1 has been precoated onto 96-well plates. Standards (NSO, L30-A212) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TNFR1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse TNFR1 amount of sample captured in plate.

Background: Tumor necrosis factor receptor 1 (TNFR1), also known as tumor necrosis factor receptor superfamily member 1A (TNFRSF1A) and CD120a, is a ubiquitous membrane receptor that binds tumor necrosis factor-alpha (TNF alpha). The protein encoded by this gene is a member of the tumor necrosis factor receptor superfamily, which also contains TNFRSF1B. And this protein is one of the major receptors for the tumor necrosis factor-alpha. This receptor can activate the transcription factor NF-kappaB, mediate apoptosis, and function as a regulator of inflammation. Antiapoptotic protein BCL2-associated athanogene 4 (BAG4/SODD) and adaptor proteins TRADD and TRAF2 have been shown to interact with this receptor, and thus play regulatory roles in the signal transduction mediated by the receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 31560799
• NCBI GeneID: 21937
• NCBI Accession #: NP_035739.2
• NCBI GenBank Nucleotide #: NM_011609.4
• UniProt Accession #: P25118
• Molecular Weight: 50,130 Da
• NCBI Official Full Name: tumor necrosis factor receptor superfamily member 1A
• NCBI Official Synonym Full Names: tumor necrosis factor receptor superfamily, member 1a
• NCBI Official Symbol: Tnfrsf1a
• NCBI Official Synonym Symbols: FPF; p55; TNF-R; TNFAR; TNFRI; Tnfr1; p55-R; CD120a; TNF-R1; TNFR60; Tnfr-2; TNF-R-I; TNF-R55; TNFRp55; TNF-alphaR1; TNFalpha-R1
• NCBI Protein Information: tumor necrosis factor receptor superfamily member 1A
• UniProt Protein Name: Tumor necrosis factor receptor superfamily member 1A
• Protein Family: Tumor necrosis factor receptor superfamily
• UniProt Gene Name: Tnfrsf1a
• UniProt Synonym Gene Names: Tnfr-1; Tnfr1; TNF-R1; TNF-RI; TNFR-I
• UniProt Entry Name: TNR1A_MOUSE

Uniprot Description

TNF-R1: Receptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate- specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase. Binding of TNF to the extracellular domain leads to homotrimerization. The aggregated death domains provide a novel molecular interface that interacts specifically with the death domain of TRADD. Various TRADD-interacting proteins such as TRAFS, RIPK1 and possibly FADD, are recruited to the complex by their association with TRADD. This complex activates at least two distinct signaling cascades, apoptosis and NF-kappa-B signaling. Interacts with BAG4, BRE, FEM1B, GRB2, SQSTM1 and TRPC4AP. Interacts with HCV core protein. Interacts with human cytomegalovirus/HHV-5 protein UL138. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, cytokine

Cellular Component: axon; cell surface; cytosol; extracellular space; Golgi apparatus; Golgi membrane; integral to membrane; integral to plasma membrane; lipid raft; membrane; mitochondrion; nucleus; plasma membrane; protein complex; receptor complex; synapse

Molecular Function: protease binding; protein binding; protein complex binding; tumor necrosis factor binding; tumor necrosis factor receptor activity

Biological Process: apoptosis; cell surface receptor linked signal transduction; cytokine and chemokine mediated signaling pathway; defense response; defense response to bacterium; DNA damage response, signal transduction resulting in induction of apoptosis; immune response; induction of apoptosis via death domain receptors; inflammatory response; multicellular organismal development; negative regulation of apoptosis; negative regulation of inflammatory response; negative regulation of interleukin-6 production; positive regulation of angiogenesis; positive regulation of inflammatory response; positive regulation of neuron apoptosis; positive regulation of protein import into nucleus, translocation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tumor necrosis factor production; positive regulation of tyrosine phosphorylation of Stat1 protein; prostaglandin metabolic process; protein heterooligomerization; regulation of apoptosis; regulation of cell proliferation; response to lipopolysaccharide; signal transduction; tumor necrosis factor-mediated signaling pathway