Typical Testing Data/Standard Curve (for reference only)
(Mouse Meteorin/METRN PicoKine ELISA Kit standard curve )
Mouse Meteorin/METRN ELISA Kit | METRN elisa kit
Mouse Meteorin/METRN PicoKine ELISA Kit
Gene Names
Metrn; Hyrac; 1810034B16Rik
Reactivity
Mouse
Synonyms
Meteorin/METRN; Mouse Meteorin/METRN PicoKine ELISA Kit; C16orf23; c380A1.2; Hyrac; Meteorin; Meteorin precursor; Metrn; Q8C1Q4 ; meteorin; glial cell differentiation regulator; METRN elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse Meteorin
Sequence Length
291
Assay Range
62.5pg/ml-4000pg/ml
Sensitivity
<12pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
15-20
Immunogen
NSO, G22-D291
Sample Data
Concentration(pg/ml): |0|62.5|125|250|500|1000|2000|4000
O.D.: |0.068|0.107|0.171|0.262|0.442|0.728|1.300|2.254
O.D.: |0.068|0.107|0.171|0.262|0.442|0.728|1.300|2.254
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |130|611|2300|152|685|2542
Standard Deviation: |6.11|33.61|144.90|10.49|52.75|213.53
CV(%): |4.7|5.5|6.3|6.9|7.7|8.4
n: |16|16|16|24|24|24
Mean(pg/ml): |130|611|2300|152|685|2542
Standard Deviation: |6.11|33.61|144.90|10.49|52.75|213.53
CV(%): |4.7|5.5|6.3|6.9|7.7|8.4
Assay Procedure Step 1
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse Meteorin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Meteorin standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse Meteorin/METRN PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse Meteorin/METRN PicoKine ELISA Kit standard curve )
Related Product Information for METRN elisa kit
For quantitative detection of mouse Meteorin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts directly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres.
Principle: Mouse Meteorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for Meteorin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G22-D291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin amount of sample captured in plate.
Background: Meteorin, glial cell differentiation regulator is a protein that in humans is encoded by the METRN gene. The mouse Metrn gene is mapped to chromosome 17. It is concluded that meteorin acts directly on satellite glial cells to induce a change in morphology and that transformed satellite glial cells induce the secondary effect of neurite extension. In cerebellar explants, meteorin induced conversion of cerebellar astrocytes into radial glia and promoted astrocyte differentiation from embryonic day 11.5 cerebral cortex neurospheres.
Principle: Mouse Meteorin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for Meteorin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: G22-D291) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Meteorin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Meteorin amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
13,797 Da
NCBI Official Full Name
meteorin
NCBI Official Synonym Full Names
meteorin, glial cell differentiation regulator
NCBI Official Symbol
Metrn
NCBI Official Synonym Symbols
Hyrac; 1810034B16Rik
NCBI Protein Information
meteorin
UniProt Protein Name
Meteorin
Protein Family
UniProt Gene Name
Metrn
UniProt Synonym Gene Names
Hyrac
Uniprot Description
METRN: Involved in both glial cell differentiation and axonal network formation during neurogenesis. Promotes astrocyte differentiation and transforms cerebellar astrocytes into radial glia. Also induces axonal extension in small and intermediate neurons of sensory ganglia by activating nearby satellite glia. Belongs to the meteorin family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 17|17 A3.3
Cellular Component: extracellular space
Biological Process: glial cell differentiation; positive regulation of axonogenesis
Research Articles on METRN
Similar Products
Product Notes
The Mouse METRN metrn (Catalog #AAA178741) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178741 ELISA Kit recognizes Mouse METRN. It is sometimes possible for the material contained within the vial of "Meteorin/METRN, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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