Mouse VEGF-B PicoKine ELISA Kit | Vegfb elisa kit

Mouse VEGF-B PicoKine ELISA Kit

Cat. Num. AAA177609

• Gene Names: Vegfb; Vrf; VEGF-B
• Reactivity: Mouse
• Synonyms: VEGF-B PicoKine; Mouse VEGF-B PicoKine ELISA Kit; Vascular endothelial growth factor B; VEGF B; VEGF related factor; VEGF-B; VEGF-related factor; Vegfb; VEGFB_HUMAN; VEGFL; VRF; ; vascular endothelial growth factor B; Vegfb elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse VEGF-B
• Sequence Length: 188

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <2pg/ml
• Sample: For quantitative detection of mouse VEGF-B in cell culture supernates, serum and plasma(heparin).
• Immunogen: sf21,Q20-A207&Q20-R148
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse VEGF-B standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin) to each empty well. We recommend that each mouse VEGF-B standard solution and each sample is measured in duplicate.
• Intra-Assay Precision: Inter-Assay Precision; Sample|1|2|3|1|2|3; n|16|16|16|24|24|24; Mean(pg/ml)|158|652|1309|197|763|1611; Standard deviation|6.95|33.25|74.61|14.58|54.94|124.05; CV(%)|4.4|5.1|5.7|7.4|7.2|7.7
• Sample Data: Concentration(pg/ml)|0|31.2|62.5|125|250|500|1000|2000; O.D.|0.006|0.122|0.245|0.441|0.743|1.101|1.748|2.241
• Anticoagulant: Heparin
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse VEGF-B PicoKine ELISA Kit standard curve )

Related Product Information for Vegfb elisa kit

Principle of the assay: Mouse VEGF-B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for VEGF-B has been precoated onto 96-well plates. Standards(sf21,Q20-A207 & Q20-R148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for VEGF-B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse VEGF-B amount of sample captured in plate.

Background: Vascular endothelial growth factor B also known as VEGF-B is a protein that, in humans, is encoded by the VEGF-B gene. VEGF-B is a growth factor that belongs to the vascular endothelial growth factor family, of which VEGF-A is the best-known member. In contrast to VEGF-A, VEGF-B plays a less pronounced role in the vascular system: Whereas VEGF-A is important for the formation of blood vessels, such as during development or in pathological conditions, VEGF-B seems to play a role only in the maintenance of newly formed blood vessels during pathological conditions. It also plays an important role on several types of neurons. And it is important for the protection of neurons in the retina and thecerebral cortex during stroke and of motoneurons during motor neuron diseases such as amyotrophic lateral sclerosis.

NCBI and Uniprot Product Information

• NCBI GI #: 297632418
• NCBI GeneID: 22340
• NCBI Accession #: NP_001172093.1
• NCBI GenBank Nucleotide #: NM_001185164.1
• UniProt Accession #: P49766; Q3UG04; Q5D0B1; Q64290
• Molecular Weight: 21,442 Da
• NCBI Official Full Name: vascular endothelial growth factor B isoform Vegf-b167
• NCBI Official Synonym Full Names: vascular endothelial growth factor B
• NCBI Official Symbol: Vegfb
• NCBI Official Synonym Symbols: Vrf; VEGF-B
• NCBI Protein Information: vascular endothelial growth factor B
• UniProt Protein Name: Vascular endothelial growth factor B
• Protein Family: Vascular endothelial growth factor
• UniProt Gene Name: Vegfb
• UniProt Synonym Gene Names: Vrf; VEGF-B; VRF
• UniProt Entry Name: VEGFB_MOUSE

Uniprot Description

VEGFB: Growth factor for endothelial cells. VEGF-B167 binds heparin and neuropilin-1 whereas the binding to neuropilin-1 of VEGF-B186 is regulated by proteolysis. Homodimer; disulfide-linked. Can also form heterodimer with VEGF. Expressed in all tissues except liver. Highest levels found in heart, skeletal muscle and pancreas. Belongs to the PDGF/VEGF growth factor family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide; Cell cycle regulation; Cytokine

Cellular Component: extracellular region; extracellular space; intracellular; membrane

Molecular Function: chemoattractant activity; growth factor activity; heparin binding; protein heterodimerization activity; protein homodimerization activity; vascular endothelial growth factor receptor 1 binding

Biological Process: angiogenesis; cardiac muscle contraction; cell differentiation; heart development; induction of positive chemotaxis; multicellular organismal development; negative regulation of apoptosis; negative regulation of neuron apoptosis; positive chemotaxis; positive regulation of cell division; positive regulation of endothelial cell proliferation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of protein kinase B signaling cascade; positive regulation of vascular endothelial growth factor receptor signaling pathway; protein amino acid O-linked glycosylation