Rat Hemojuvelin/RGM-C ELISA Kit | HFE2 elisa kit

Rat Hemojuvelin/RGM-C PicoKine ELISA Kit

Cat. Num. AAA178726

• Gene Names: Hfe2; Rgmc
• Reactivity: Rat
• Synonyms: Hemojuvelin/RGM-C; Rat Hemojuvelin/RGM-C PicoKine ELISA Kit; Haemojuvelin; HEMOCHROMATOSIS; PEPTIDE; Hemojuvelin; HFE 2; HFE2; HFE2A; HJV; JH; Juvenile; RGMC; RGM C; Q6ZVN8; hemochromatosis type 2 (juvenile); HFE2 elisa kit

• Reactivity: Rat
• Specificity: Natural and recombinant rat Hemojuvelin
• Sequence Length: 422

• Assay Range: 62.5pg/ml-4000pg/ml
• Sensitivity: <12pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 15-20
• Immunogen: NSO, Q36-D396
• Sample Data: Concentration(pg/ml): |0|62.5|125|250|500|1000|2000|4000; O.D.: |0.019|0.060|0.092|0.178|0.406|0.799|1.611|2.283
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |157|601|2210|180|712|2746; Standard Deviation: |6.75|33.66|130.39|11.52|52.69|233.41; CV(%): |4.3|5.6|5.9|6.4|7.4|8.5
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml rat Hemojuvelin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat Hemojuvelin standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Rat Hemojuvelin/RGM-C PicoKine ELISA Kit standard curve )

Related Product Information for HFE2 elisa kit

For quantitative detection of rat Hemojuvelin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Hemojuvelin (HJV/RGMc/HFE2) is a membrane-bound and soluble protein in mammals. It is a member of the repulsive guidance molecule family of proteins. The product of this gene is involved in iron metabolism. It may be a component of the signaling pathway which activates hepcidin or it may act as a modulator of hepcidin expression. It could also represent the cellular receptor for hepcidin. Two uORFs in the 5' UTR negatively regulate the expression and activity of the encoded protein. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. Defects in this gene are the cause of hemochromatosis type 2A, also called juvenile hemochromatosis (JH). JH is an early-onset autosomal recessive disorder due to severe iron overload resulting in hypogonadotrophic hypogonadism, hepatic fibrosis or cirrhosis and cardiomyopathy, occurring typically before age of 30.
Principle: Rat Hemojuvelin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Hemojuvelin has been precoated onto 96-well plates. Standards(Expression system for standard: NSO, Immunogen sequence: Q36-D396) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Hemojuvelin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Hemojuvelin amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 51316136
• NCBI GeneID: 310681
• UniProt Accession #: Q8N7M5
• Molecular Weight: 45,221 Da
• NCBI Official Full Name: Hemojuvelin
• NCBI Official Synonym Full Names: hemochromatosis type 2 (juvenile)
• NCBI Official Symbol: Hfe2
• NCBI Official Synonym Symbols: Rgmc
• NCBI Protein Information: hemojuvelin
• UniProt Protein Name: Hemojuvelin
• Protein Family: Hemojuvelin
• UniProt Gene Name: Hfe2
• UniProt Synonym Gene Names: Rgmc

Uniprot Description

Acts as a bone morphogenetic protein (BMP) coreceptor. Through enhancement of BMP signaling regulates hepcidin (HAMP) expression and regulates iron homeostasis.