Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL27/CTACK PicoKine ELISA Kit standard curve )
Mouse CCL27/CTACK ELISA Kit | CCL27 elisa kit
Mouse CCL27/CTACK PicoKine ELISA Kit
Reactivity
Mouse
Synonyms
CCL27/CTACK; Mouse CCL27/CTACK PicoKine ELISA Kit; CC chemokine ILC; CCL27; CTACK; ESkine; IL-11 R-alpha-locus chemokine; ILC; SCYA27; Q9Y4X3; Skinkine; Small inducible cytokine A27; Small-inducible cytokine A27; C-C motif chemokine 27; chemokine (C-C motif) ligand 27; CCL27 elisa kit
Reactivity
Mouse
Specificity
Natural and recombinant mouse CCL27
Sequence Length
120
Assay Range
62.5pg/ml-4000pg/ml
Sensitivity
<12pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
15-20
Immunogen
E Coli, L26-N120
Sample Data
Concentration(pg/ml): |0|62.5|125|250|500|1000|2000|4000
O.D.: |0.027|0.100|0.139|0.226|0.420|0.775|1.300|1.906
O.D.: |0.027|0.100|0.139|0.226|0.420|0.775|1.300|1.906
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |158|600|2301|192|721|2587
Standard Deviation: |7.27|33|135.76|11.71|48.31|191.44
CV(%): |4.6|5.5|5.9|6.1|6.7|7.4
n: |16|16|16|24|24|24
Mean(pg/ml): |158|600|2301|192|721|2587
Standard Deviation: |7.27|33|135.76|11.71|48.31|191.44
CV(%): |4.6|5.5|5.9|6.1|6.7|7.4
Assay Procedure Step 1
Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse CCL27 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum, plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL27 standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL27/CTACK PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Mouse CCL27/CTACK PicoKine ELISA Kit standard curve )
Related Product Information for CCL27 elisa kit
For quantitative detection of mouse CCL27 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). The human gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. And CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
Principle: Mouse CCL27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for CCL27 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: L26-N120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CCL27 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 27 (CCL27) is a small cytokine belonging to the CC chemokine family, it is also known under the names IL-11 R-alpha-locus chemokine (ILC), Skinkine, ESkine and Cutaneous T-cell-attracting chemokine (CTACK). The human gene is mapped to 9p13.3. CCL27 is associated with homing of memory T lymphocytes to the skin, and plays a role in T cell-mediatedinflammation of the skin. And CCL27 is expressed in numerous tissues, including gonads, thymus, placenta and skin. It elicits its chemotacticeffects by binding to the chemokine receptor CCR10.
Principle: Mouse CCL27 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for CCL27 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: L26-N120) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL27 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CCL27 amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
15,774 Da
NCBI Official Full Name
chemokine (C-C motif) ligand 27b isoform 2
NCBI Official Synonym Full Names
predicted gene 13306
NCBI Official Symbol
Gm13306
NCBI Protein Information
chemokine (C-C motif) ligand 27b
UniProt Protein Name
C-C motif chemokine 27
Protein Family
UniProt Gene Name
Ccl27
UniProt Synonym Gene Names
Ilc; Scya27; CTACK; ALP; mILC
Uniprot Description
CCL27: Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10. Belongs to the intercrine beta (chemokine CC) family.
Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 4 A5|4 22.08 cM
Cellular Component: extracellular region; nucleus
Molecular Function: cytokine activity; protein binding
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Product Notes
The Mouse CCL27 ccl27 (Catalog #AAA178719) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178719 ELISA Kit recognizes Mouse CCL27. It is sometimes possible for the material contained within the vial of "CCL27/CTACK, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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