Mouse CCL25/TECK ELISA Kit | Ccl25 elisa kit

Mouse CCL25/TECK PicoKine ELISA Kit

Cat. Num. AAA178730

• Gene Names: Ccl25; TECK; CKb15; Scya25; AI852536; A130072A22Rik
• Reactivity: Mouse
• Synonyms: CCL25/TECK; Mouse CCL25/TECK PicoKine ELISA Kit; CCL25; Chemokine TECK; O15444; SCY A25; SCYA25; TECK; Small inducible cytokine A25; Small-inducible cytokine A25; TECKvar; Thymus-expressed chemokine; C-C motif chemokine 25; chemokine (C-C motif) ligand 25; Ccl25 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse CCL25
• Sequence Length: 144

• Assay Range: 31.2pg/ml-2000pg/ml
• Sensitivity: <10pg/ml
• Anticoagulant: heparin or EDTA
• TMB incubation time: 25-30
• Immunogen: E Coli, Q24-N144
• Sample Data: Concentration(pg/ml): |0|31.2|62.5|125|250|500|1000|2000; O.D.: |0.006|0.046|0.093|0.187|0.345|0.743|1.247|2.004
• Intra/Inter Assay Precision: Sample: |1|2|3|1|2|3; n: |16|16|16|24|24|24; Mean(pg/ml): |50.1|133|992|43.8|221|675; Standard Deviation: |2.20|6.38|57.53|2.27|13.92|51.30; CV(%): |4.4|4.8|5.8|5.2|6.3|7.6
• Assay Procedure Step 1: Aliquot 0.1ml per well of the 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse CCL25 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each mouse CCL25 standard solution and each sample be measured in duplicate.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

(Mouse CCL25/TECK PicoKine ELISA Kit standard curve )

Related Product Information for Ccl25 elisa kit

For quantitative detection of mouse CCL25 in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Chemokine (C-C motif) ligand 25 (CCL25) is a small cytokine belonging to the CC chemokine family that is also known as TECK (Thymus-Expressed Chemokine). The mouse Teck gene was mapped to chromosome 8 and human Teck gene was to 19p13.2. CCL25 is believed to play a role in the development of T-cells. It is chemotactic for thymocytes, macrophages, and dendritic cells. CCL25 elicits its effects by binding to the chemokine receptor CCR9.
Principle: Mouse CCL25 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Rat specific for CCL25 has been precoated onto 96-well plates. Standards(Expression system for standard: E Coli, Immunogen sequence: Q24-N144) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL25 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse CCL25 amount of sample captured in plate.

NCBI and Uniprot Product Information

• NCBI GI #: 6677881
• NCBI GeneID: 20300
• NCBI Accession #: NP_033164.1
• NCBI GenBank Nucleotide #: NM_009138.3
• UniProt Accession #: O35903; Q9QYY6
• Molecular Weight: 16,733 Da
• NCBI Official Full Name: C-C motif chemokine 25
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 25
• NCBI Official Symbol: Ccl25
• NCBI Official Synonym Symbols: TECK; CKb15; Scya25; AI852536; A130072A22Rik
• NCBI Protein Information: C-C motif chemokine 25
• UniProt Protein Name: C-C motif chemokine 25
• Protein Family: C-C motif chemokine
• UniProt Gene Name: Ccl25
• UniProt Synonym Gene Names: Scya25; Teck

Uniprot Description

CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Chemokine; Motility/polarity/chemotaxis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8 A1.1|8 2.01 cM

Cellular Component: extracellular space

Molecular Function: CCR chemokine receptor binding; CCR10 chemokine receptor binding; chemokine activity; chemokine receptor binding; protein binding

Biological Process: chemotaxis; G-protein coupled receptor protein signaling pathway; inflammatory response; leukocyte migration; lymphocyte chemotaxis; monocyte chemotaxis; neutrophil chemotaxis; positive regulation of cell-matrix adhesion; positive regulation of GTPase activity