Typical Testing Data/Standard Curve (for reference only)
(Rat Sclerostin/SOST PicoKine ELISA Kit standard curve )
Rat Sclerostin/SOST ELISA Kit | SOST elisa kit
Rat Sclerostin/SOST PicoKine ELISA Kit
Reactivity
Rat
Synonyms
Sclerostin/SOST; Rat Sclerostin/SOST PicoKine ELISA Kit; Q9BQB4; Sclerostin; SOST; SOST elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat Sclerostin
Sequence Length
213
Assay Range
15.6pg/ml-1000pg/ml
Sensitivity
<10pg/ml
Anticoagulant
heparin or EDTA
TMB incubation time
15-20
Immunogen
NSO, F29-Y213
Sample Data
Concentration(pg/ml): |0|15.6|31.2|62.5|125|250|500|1000
O.D.: |0.028|0.069|0.125|0.215|0.381|0.729|1.433|2.243
O.D.: |0.028|0.069|0.125|0.215|0.381|0.729|1.433|2.243
Intra/Inter Assay Precision
Sample: |1|2|3|1|2|3
n: |16|16|16|24|24|24
Mean(pg/ml): |40.2|130|528|47.3|142|641
Standard Deviation: |1.65|6.11|27.98|2.60|10.08|41.02
CV(%): |4.1|4.7|5.3|5.5|7.1|6.4
n: |16|16|16|24|24|24
Mean(pg/ml): |40.2|130|528|47.3|142|641
Standard Deviation: |1.65|6.11|27.98|2.60|10.08|41.02
CV(%): |4.1|4.7|5.3|5.5|7.1|6.4
Assay Procedure Step 1
Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.3pg/ml, 15.6pg/ml rat Sclerostin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernates, serum or plasma (heparin, EDTA) to each empty well. It is recommended that each rat Sclerostin standard solution and each sample be measured in duplicate.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles.
Typical Testing Data/Standard Curve (for reference only)
(Rat Sclerostin/SOST PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat Sclerostin/SOST PicoKine ELISA Kit standard curve )
Related Product Information for SOST elisa kit
For quantitative detection of rat Sclerostin in cell culture supernates, cell lysates, tissue homogenates, serum and plasma (heparin, EDTA).
Background: Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
Principle: Rat Sclerostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Sclerostin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: F29-Y213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Sclerostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Sclerostin amount of sample captured in plate.
Background: Sclerostin is a protein that in humans is encoded by the SOST gene. It is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Additionally, Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21.
Principle: Rat Sclerostin ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from Mouse specific for Sclerostin has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: F29-Y213) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Sclerostin is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substRate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Sclerostin amount of sample captured in plate.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
23,974 Da
NCBI Official Full Name
sclerostin
NCBI Official Synonym Full Names
sclerostin
NCBI Official Symbol
Sost
NCBI Protein Information
sclerostin
UniProt Protein Name
Sclerostin
Protein Family
UniProt Gene Name
Sost
NCBI Description
may act as a regulator of bone homeostasis [RGD, Feb 2006]
Uniprot Description
SOST: Negative regulator of bone growth that acts through inhibition of Wnt signaling and bone formation
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 10q32.1
Cellular Component: extracellular matrix; extracellular space; Golgi apparatus; protein complex
Molecular Function: protein binding; transcription factor binding
Biological Process: negative regulation of BMP signaling pathway; negative regulation of ossification; negative regulation of protein complex assembly; negative regulation of Wnt receptor signaling pathway; ossification; positive regulation of transcription, DNA-dependent; response to mechanical stimulus
Research Articles on SOST
Similar Products
Product Notes
The Rat SOST sost (Catalog #AAA178751) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA178751 ELISA Kit recognizes Rat SOST. It is sometimes possible for the material contained within the vial of "Sclerostin/SOST, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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