Rabbit RANKL Polyclonal Antibody | anti-TNFSF11 antibody

Anti-RANKL Antibody

Cat. Num. AAA178515

• Gene Names: Tnfsf11; RANKL
• Reactivity: Mouse, Rat; No cross reactivity with other proteins.
• Applications: Western Blot, ELISA
• Purity: Immunogen affinity purified.
• Synonyms: RANKL; Polyclonal Antibody; Anti-RANKL Antibody; CD254; hRANKL2; ODF; OPGL; OPTB2; Osteoprotegerin ligand; sOdf; TNFSF 11; TNFSF11; TRANCE; Q9ESE2; Tumor necrosis factor ligand superfamily member 11; tumor necrosis factor superfamily member 11; anti-TNFSF11 antibody

• Host: Rabbit
• Reactivity: Mouse, Rat; No cross reactivity with other proteins.
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Immunogen affinity purified.
• Form/Format: Lyophilized
• Sequence Length: 318

• Applicable Applications for anti-TNFSF11 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Western Blot: 0.1-0.5mug/ml; Tested Species: Rat; ELISA: 0.1-0.5mug/ml; Tested Species: Mouse; Predicted Species: Rat; Tested Species:In-house tested species with positive results.; Predicted Species: Species predicted to be fit for the product based on sequence similarities.; Other applications have not been tested.
• Immunogen: E Coli-derived rat RANKL recombinant protein (Position: F141-D318). Rat RANKL shares 87.9% and 96.6% amino acid (aa) sequence identity with human and mouse RANKL, respectively.
• Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
• Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Preparation and Storage: At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquotted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.

Western Blot (WB)

(Western blot analysis of RANKL expression in rat brain extract (lane 1). RANKL at 40KD was detected using rabbit anti- RANKL Antigen Affinity purified polyclonal antibody at 0.5 ug/mL. The blot was developed using chemiluminescence (ECL) method. )

Related Product Information for anti-TNFSF11 antibody

Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 11 (TNFSF11) detection.
Background: Receptor activator of nuclear factor kappa-B ligand (RANKL), also known as tumor necrosis factor ligand superfamily member 11 (TNFSF11), is a protein that in humans is encoded by the TNFSF11 gene. This gene encodes a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. It is mapped to chromosome13q14.11. Targeted disruption of the related gene in mice led to severe osteopetrosis and a lack of osteoclasts. The deficient mice exhibited defects in early differentiation of T and B lymphocytes, and failed to form lobulo-alveolar mammary structures during pregnancy. This gene may play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy.

References

1. Asselin-Labat, M.-L., Vaillant, F., Sheridan, J. M., Pal, B., Wu, D., Simpson, E. R. Yasuda, H.; Smyth, G. K.; Martin, T. J.; Lindeman, G. J.; Visvader, J. E.: Control of mammary stem cell function by steroid hormone signalling. Nature 465: 798-802, 2010.
2. Luan, X., Lu, Q., Jiang, Y., Zhang, S., Wang, Q., Yuan, H., Zhao, W., Wang, J., Wang, X.Crystal structure of human RANKL complexed with its decoy receptor osteoprotegerin. J Immun. 189: 245-252, 2012.

NCBI and Uniprot Product Information

• NCBI GI #: 16924012
• NCBI GeneID: 117516
• NCBI Accession #: NP_476490.1
• NCBI GenBank Nucleotide #: NM_057149.1
• UniProt Accession #: Q9ESE2; Q91ZI9
• Molecular Weight: 35,370 Da
• NCBI Official Full Name: tumor necrosis factor ligand superfamily member 11
• NCBI Official Synonym Full Names: tumor necrosis factor superfamily member 11
• NCBI Official Symbol: Tnfsf11
• NCBI Official Synonym Symbols: RANKL
• NCBI Protein Information: tumor necrosis factor ligand superfamily member 11
• UniProt Protein Name: Tumor necrosis factor ligand superfamily member 11
• Protein Family: Tumor necrosis factor ligand superfamily
• UniProt Gene Name: Tnfsf11
• UniProt Synonym Gene Names: Opgl; Rankl; Trance; ODF; OPGL; RANKL; TRANCE
• UniProt Entry Name: TNF11_RAT

NCBI Description

induces osteoclast formation and bone resoprtion; plays a role in regulation of calcium homeostasis and osteoclast function [RGD, Feb 2006]

Uniprot Description

Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.